2020
A cut above (and below): Protein cleavage in the regulation of polycystin trafficking and signaling
Padovano V, Mistry K, Merrick D, Gresko N, Caplan MJ. A cut above (and below): Protein cleavage in the regulation of polycystin trafficking and signaling. Cellular Signalling 2020, 72: 109634. PMID: 32283256, PMCID: PMC7269866, DOI: 10.1016/j.cellsig.2020.109634.Peer-Reviewed Original ResearchConceptsPolycystin-1Polycystin proteinsG proteinsPolycystin-1 proteinProtein maturationTerminal tailObligate stepBiological pathwaysProtein cleavagePhysiological functionsProteolytic siteProteinPathological consequencesAutosomal dominant polycystic kidney diseaseTraffickingDominant polycystic kidney diseasePolycystic kidney diseasePrimary functionCleavageRegulationMaturationGenesMitochondriaValuable insightsPathway
2010
MAL/VIP17, a New Player in the Regulation of NKCC2 in the Kidney
Carmosino M, Rizzo F, Procino G, Basco D, Valenti G, Forbush B, Schaeren-Wiemers N, Caplan MJ, Svelto M. MAL/VIP17, a New Player in the Regulation of NKCC2 in the Kidney. Molecular Biology Of The Cell 2010, 21: 3985-3997. PMID: 20861303, PMCID: PMC2982131, DOI: 10.1091/mbc.e10-05-0456.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell LineEndocytosisEpithelial CellsHumansImmunoprecipitationKidneyLLC-PK1 CellsMembrane Transport ProteinsMiceMice, TransgenicMyelin and Lymphocyte-Associated Proteolipid ProteinsMyelin ProteinsPhosphorylationProtein BindingProteolipidsRatsRats, Inbred WKYRNA InterferenceSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 1SwineConceptsRegulation of NKCC2Apical membraneMajor salt transport pathwayC-terminal tailCell surface retentionApical sortingPorcine kidney cellsCotransporter phosphorylationTransgenic mice resultsNephron structuresRegulated absorptionImportant roleNew playersKidney cellsSurface expressionMice resultsSurface retentionTransport pathwaysNKCC2MembraneRegulationLymphocyte-associated proteinCyst formationRat kidney medullaColocalize
2008
Chapter 4 Protein Trafficking in Polarized Cells
Duffield A, Caplan MJ, Muth TR. Chapter 4 Protein Trafficking in Polarized Cells. International Review Of Cytology 2008, 270: 145-179. PMID: 19081536, DOI: 10.1016/s1937-6448(08)01404-4.Peer-Reviewed Original ResearchConceptsEpithelial cellsProtein traffickingBasolateral membrane surfaceLipid traffickingPolarized cellsDynamic regulationSpecific lipidsAdjacent epithelial cellsCell typesBasolateral surfaceApical surfaceCertain cellsMembrane surfaceTraffickingProtein contentCellsSortingNumber of sortingsImmune systemRecent advancesMechanismProteinRegulationEpitheliumLipids
2007
The Future of the Pump
Caplan MJ. The Future of the Pump. Journal Of Clinical Gastroenterology 2007, 41: s217-s222. PMID: 17575526, DOI: 10.1097/mcg.0b013e31803233da.Peer-Reviewed Original ResearchConceptsIon transport proteinsLarge macromolecular complexesMacromolecular complexesGastric parietal cellsTransport proteinsSecond messengerMacromolecular interactionsIon translocationRegulatory processesK-ATPaseCritical roleTight controlX-ray crystallographic techniquesParietal cellsCrystallographic techniquesCellsKinasePharmacologic suppressionTraffickingProteinTranslocationMessengerMolecular structureRegulationSecretion
2005
The C-Terminal Tail of the Polycystin-1 Protein Interacts with the Na,K-ATPase α-Subunit
Zatti A, Chauvet V, Rajendran V, Kimura T, Pagel P, Caplan MJ. The C-Terminal Tail of the Polycystin-1 Protein Interacts with the Na,K-ATPase α-Subunit. Molecular Biology Of The Cell 2005, 16: 5087-5093. PMID: 16107561, PMCID: PMC1266409, DOI: 10.1091/mbc.e05-03-0200.Peer-Reviewed Original ResearchConceptsC-terminal tailPolycystin-1Cytoplasmic C-terminal tailK-ATPase α-subunitPolycystin-1 proteinK-ATPase activityRegulation of NaChinese hamster ovary cellsProtein interactsHamster ovary cellsProtein exhibitΑ-subunitFunctional studiesAmino acidsPKD1 geneOvary cellsAutosomal dominant polycystic kidney diseaseDominant polycystic kidney diseasePolycystic kidney diseaseInteractsKinetic propertiesRegulationGenesTailProtein
2004
Gastric parietal cell acid secretion in mice can be regulated independently of H+/K+ ATPase endocytosis
Nguyen NV, Gleeson PA, Courtois-Coutry N, Caplan MJ, van Driel IR. Gastric parietal cell acid secretion in mice can be regulated independently of H+/K+ ATPase endocytosis. Gastroenterology 2004, 127: 145-154. PMID: 15236181, DOI: 10.1053/j.gastro.2004.04.016.Peer-Reviewed Original ResearchConceptsApical plasma membranePlasma membraneIntracellular traffickingTyrosine-based endocytosis motifATPase activityATPase beta subunitMembrane traffickingATPase endocytosisTrafficking eventsEndocytosis motifParietal cell ultrastructureTubulovesicular compartmentCytoplasmic tailIntracytoplasmic compartmentCl- conductanceParietal cell acid secretionBeta subunitParietal cellsDirect regulationProton pumpCell ultrastructureTraffickingATPaseCellsRegulation
2003
TRANSPORT PROTEIN TRAFFICKING IN POLARIZED CELLS
Muth TR, Caplan MJ. TRANSPORT PROTEIN TRAFFICKING IN POLARIZED CELLS. Annual Review Of Cell And Developmental Biology 2003, 19: 333-366. PMID: 14570573, DOI: 10.1146/annurev.cellbio.19.110701.161425.Peer-Reviewed Original ResearchConceptsTransport proteinsMembrane trafficking processesIon transport proteinsIon transport activityProtein traffickingCellular machineryTrafficking processesPolarized cellsPlasma membraneEndocytic retrievalSubcellular distributionPhysiological functionsTransport activityCell surfaceBiochemical natureCell membraneIntracellular populationProteinMembraneTraffickingMachineryRegulationCellsDomainIon Pump‐Interacting Proteins: Promising New Partners
PAGEL P, ZATTI A, KIMURA T, DUFFIELD A, CHAUVET V, RAJENDRAN V, CAPLAN MJ. Ion Pump‐Interacting Proteins: Promising New Partners. Annals Of The New York Academy Of Sciences 2003, 986: 360-368. PMID: 12763851, DOI: 10.1111/j.1749-6632.2003.tb07215.x.Peer-Reviewed Original Research
2001
Ion Pumps in Polarized Cells: Sorting and Regulation of the Na+,K+- and H+,K+-ATPases*
Dunbar L, Caplan M. Ion Pumps in Polarized Cells: Sorting and Regulation of the Na+,K+- and H+,K+-ATPases*. Journal Of Biological Chemistry 2001, 276: 29617-29620. PMID: 11404365, DOI: 10.1074/jbc.r100023200.Peer-Reviewed Original ResearchConceptsP-type familyIon transport proteinsDistinct regulatory pathwaysSubcellular localizationPolarized cellsRelated membersRegulatory pathwaysTransport proteinsMolecular signalsATPasesCellular mechanismsIon pumpsEnzymatic activityEpithelial cellsProteinComplex arrayCatalytic capacityPhysiologic functionIntramolecular interactionsCellsHomologyTraffickingATPasePathwayRegulation
2000
The cell biology of ion pumps: sorting and regulation
Dunbar L, Caplan M. The cell biology of ion pumps: sorting and regulation. European Journal Of Cell Biology 2000, 79: 557-563. PMID: 11001492, DOI: 10.1078/0171-9335-00079.Peer-Reviewed Original ResearchConceptsP-type familyPolarized epithelial cellsIon pumpsK-ATPaseDistinct regulatory pathwaysProtein traffickingSubcellular localizationCell biologyRelated membersRegulatory pathwaysMolecular signalsCellular mechanismsEnzymatic activityIntra-molecular interactionsEpithelial cellsTraffickingComplex arrayCatalytic capacityPhysiologic functionATPasesHomologyBiologyPathwayRegulationSorting
1998
Gastric H+/K+-ATPase: targeting signals in the regulation of physiologic function
Caplan M. Gastric H+/K+-ATPase: targeting signals in the regulation of physiologic function. Current Opinion In Cell Biology 1998, 10: 468-473. PMID: 9719867, DOI: 10.1016/s0955-0674(98)80060-4.Peer-Reviewed Original Research
1997
Sorting and trafficking of ion transport proteins in polarized epithelial cells
Muth T, Dunbar L, Cortois-Coutry N, Roush D, Caplan M. Sorting and trafficking of ion transport proteins in polarized epithelial cells. Current Opinion In Nephrology & Hypertension 1997, 6: 455-459. PMID: 9327204, DOI: 10.1097/00041552-199709000-00008.Peer-Reviewed Original Research
1995
Na+,K+-ATPase in the Choroid Plexus REGULATION BY SEROTONIN/PROTEIN KINASE C PATHWAY (∗)
Fryckstedt J, Caplan M, Aperia A, Fisone G, Snyder G, Greengard P. Na+,K+-ATPase in the Choroid Plexus REGULATION BY SEROTONIN/PROTEIN KINASE C PATHWAY (∗). Journal Of Biological Chemistry 1995, 270: 2427-2430. PMID: 7852300, DOI: 10.1074/jbc.270.6.2427.Peer-Reviewed Original ResearchConceptsProtein kinase CKinase CTwo-dimensional peptide mappingProtein kinase C pathwayKinase C pathwayProtein phosphorylationFirst messengersIntact cellsIon pumpsPeptide mappingATPaseC pathwayPhosphorylationPhorbolDemonstrated abilityMessengerComigrationActivatorRegulationPathwayActivityChoroid plexusMechanismProductionTurnover