2017
The antimalarial activity of the pantothenamide α-PanAm is via inhibition of pantothenate phosphorylation
Chiu JE, Thekkiniath J, Choi JY, Perrin BA, Lawres L, Plummer M, Virji AZ, Abraham A, Toh JY, Zandt MV, Aly ASI, Voelker DR, Mamoun CB. The antimalarial activity of the pantothenamide α-PanAm is via inhibition of pantothenate phosphorylation. Scientific Reports 2017, 7: 14234. PMID: 29079738, PMCID: PMC5660193, DOI: 10.1038/s41598-017-14074-9.Peer-Reviewed Original ResearchConceptsMode of actionBlood stage developmentPantothenate kinase activityYeast mutantsMass spectrometry analysisBlood-stage parasitesPA-dependent mannerKinase activityPantothenate phosphorylationCAB1Pantothenate kinaseStage parasitesYeast growthCoenzyme ABiochemical analysisBlood stagesDirect competitionPantothenic acidSpectrometry analysisResistant P. falciparum strainsPhosphorylationStage developmentPlasmodium falciparumCompetitive inhibitorP. berghei
2014
Plasmodium yoelii Vitamin B5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the Mosquito
Hart RJ, Lawres L, Fritzen E, Mamoun C, Aly AS. Plasmodium yoelii Vitamin B5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the Mosquito. Scientific Reports 2014, 4: 5665. PMID: 25012929, PMCID: PMC4092334, DOI: 10.1038/srep05665.Peer-Reviewed Original ResearchConceptsSexual blood stage developmentYeast cellsLife cycle stagesNon-photosynthetic cellsParasite plasma membraneMultiple parasite life cycle stagesParasite life cycle stagesBlood stage developmentPantothenate transportGene-targeting techniquesHuman malaria parasiteCycle stagesFunctional complementationPAT inhibitorsPlasma membraneSporozoite formationTransporter candidatesVivo functionOocyst stageP. falciparuminParasite transmissionPantothenate concentrationPantothenate uptakeCoenzyme AMalaria parasites