2024
Traumatic brain injury disrupts state-dependent functional cortical connectivity in a mouse model
Bottom-Tanzer S, Corella S, Meyer J, Sommer M, Bolaños L, Murphy T, Quiñones S, Heiney S, Shtrahman M, Whalen M, Oren R, Higley M, Cardin J, Noubary F, Armbruster M, Dulla C. Traumatic brain injury disrupts state-dependent functional cortical connectivity in a mouse model. Cerebral Cortex 2024, 34: bhae038. PMID: 38365273, DOI: 10.1093/cercor/bhae038.Peer-Reviewed Original ResearchConceptsControlled cortical impactTraumatic brain injuryFunctional connectivityDisrupted functional connectivityAssociated with improved cognitionReduced theta powerBrain injuryHuman TBI patientsModel of traumatic brain injuryBehavioral state-dependent changesNetwork connectivity changesFunctional cortical connectivityBrain regionsTheta powerConnectivity changesState-dependent changesCortical impactPeriods of locomotionCortical connectivityTBI patientsRodent modelsECoG activityMotor dysfunctionCortexInjured cortex
2023
The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo
Yu C, Yu Y, Adsit L, Chang J, Barchini J, Moberly A, Benisty H, Kim J, Young B, Heng K, Farinella D, Leikvoll A, Pavan R, Vistein R, Nanfito B, Hildebrand D, Otero-Coronel S, Vaziri A, Goldberg J, Ricci A, Fitzpatrick D, Cardin J, Higley M, Smith G, Kara P, Nielsen K, Smith I, Smith S. The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo. Nature Methods 2023, 21: 132-141. PMID: 38129618, PMCID: PMC10776402, DOI: 10.1038/s41592-023-02098-1.Peer-Reviewed Original ResearchRapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior
Benisty H, Barson D, Moberly A, Lohani S, Tang L, Coifman R, Crair M, Mishne G, Cardin J, Higley M. Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior. Nature Neuroscience 2023, 27: 148-158. PMID: 38036743, PMCID: PMC11316935, DOI: 10.1038/s41593-023-01498-y.Peer-Reviewed Original ResearchConceptsFunctional connectivitySpontaneous behaviorCortical networksCortical network activityTime-varying functional connectivityFunctional magnetic resonanceCerebral cortexAwake miceDynamic functional connectivityAwake animalsNeighboring neuronsPatterned activityDistinct behavioral statesTwo-photon microscopyNeural activityCortical signalsBehavioral statesCortexNetwork activityCortical dynamicsMagnetic resonanceVIP interneurons regulate cortical size tuning and visual perception
Ferguson K, Salameh J, Alba C, Selwyn H, Barnes C, Lohani S, Cardin J. VIP interneurons regulate cortical size tuning and visual perception. Cell Reports 2023, 42: 113088. PMID: 37682710, PMCID: PMC10618959, DOI: 10.1016/j.celrep.2023.113088.Peer-Reviewed Original ResearchConceptsState-dependent modulationPyramidal neuronsVIP-INsBehavioral state-dependent modulationCortical circuit functionVasoactive intestinal peptidePrimary visual cortexAwake behaving miceIntestinal peptideGABAergic interneuronsVIP interneuronsCortical activityVisual cortexBehaving miceFeature selectivityInterneuronsSensory processingSpecialized populationCircuit functionStimulus sizeActivity altersDiverse populationsModulationPopulationCortexDevelopmental loss of ErbB4 in PV interneurons disrupts state-dependent cortical circuit dynamics
Batista-Brito R, Majumdar A, Nuño A, Ward C, Barnes C, Nikouei K, Vinck M, Cardin J. Developmental loss of ErbB4 in PV interneurons disrupts state-dependent cortical circuit dynamics. Molecular Psychiatry 2023, 28: 3133-3143. PMID: 37069344, PMCID: PMC10618960, DOI: 10.1038/s41380-023-02066-3.Peer-Reviewed Original ResearchConceptsPV interneuronsCortical neuronsCortical circuitsCortical circuit dynamicsCortical GABAergic cellsNRG1/ErbB4Excitatory cortical neuronsParvalbumin-Expressing InterneuronsInhibitory cortical neuronsApical dendritic tuftsActivity of excitatorySecond postnatal weekProper synaptic connectivityLate postnatal developmentState-dependent modulationLoss of ERBB4Excitatory componentGABAergic cellsGABAergic inhibitionSpine densityDendritic tuftsPostnatal weekNormal tuningSynaptic connectivityReceptor ErbB4
2022
Spatiotemporally heterogeneous coordination of cholinergic and neocortical activity
Lohani S, Moberly A, Benisty H, Landa B, Jing M, Li Y, Higley M, Cardin J. Spatiotemporally heterogeneous coordination of cholinergic and neocortical activity. Nature Neuroscience 2022, 25: 1706-1713. PMID: 36443609, PMCID: PMC10661869, DOI: 10.1038/s41593-022-01202-6.Peer-Reviewed Original ResearchConceptsCortical network activityBehavioral statesCholinergic modulationAnimal's behavioral stateCholinergic signalingAwake miceCortical functionFunctional reorganizationNeuromodulatory influencesDifferent motor behaviorsNeocortical activityAcetylcholineMotor behaviorCortical networksRecent evidenceBrain activityFunctional segregationNeocortexMesoscopic imagingHeterogeneous signalsNetwork activityBehavioral markersBehavioral variablesCircuit dynamicsCholinergicDual-polarity voltage imaging of the concurrent dynamics of multiple neuron types
Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin J, Schnitzer M, Pieribone V. Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types. Science 2022, 378: eabm8797. PMID: 36378956, PMCID: PMC9703638, DOI: 10.1126/science.abm8797.Peer-Reviewed Original Research
2018
Altered hippocampal interneuron activity precedes ictal onset
Miri ML, Vinck M, Pant R, Cardin J. Altered hippocampal interneuron activity precedes ictal onset. ELife 2018, 7: e40750. PMID: 30387711, PMCID: PMC6245730, DOI: 10.7554/elife.40750.Peer-Reviewed Original ResearchConceptsSynaptic inhibitionIctal activityEarly ictal phaseHippocampal interneuron activityLocal circuit interactionsSomatostatin-expressing (SST) interneuronsInhibitory interneuron populationsSeizure inductionGABAergic inhibitionSeizure disorderIctal onsetSource of inhibitionSeizure initiationIctal phaseHippocampal circuitsInterneuron activityInterneuron populationsInterneuron classesFiring patternsInterneuronsElectrophysiological approachesPreictal periodInhibitionParvalbuminOnset
2016
Projection-Specific Visual Feature Encoding by Layer 5 Cortical Subnetworks
Lur G, Vinck MA, Tang L, Cardin JA, Higley MJ. Projection-Specific Visual Feature Encoding by Layer 5 Cortical Subnetworks. Cell Reports 2016, 14: 2538-2545. PMID: 26972011, PMCID: PMC4805451, DOI: 10.1016/j.celrep.2016.02.050.Peer-Reviewed Original ResearchConceptsNeocortical sensory areasVisual response propertiesPrimary visual cortexVivo calcium imagingDownstream targetsCorticocortical cellsCorticostriatal cellsProjection neuronsRelevant downstream targetsCorticotectal cellsAfferent informationCortical subnetworksCC cellsVisual cortexCalcium imagingSensory areasSubcortical structuresLayer 5CT cellsDistinct subpopulationsSensory informationBroad tuningResponse propertiesCellsFunctional subnetworks
2015
Optogenetic stimulation of cholinergic brainstem neurons during focal limbic seizures: Effects on cortical physiology
Furman M, Zhan Q, McCafferty C, Lerner BA, Motelow JE, Meng J, Ma C, Buchanan GF, Witten IB, Deisseroth K, Cardin JA, Blumenfeld H. Optogenetic stimulation of cholinergic brainstem neurons during focal limbic seizures: Effects on cortical physiology. Epilepsia 2015, 56: e198-e202. PMID: 26530287, PMCID: PMC4679683, DOI: 10.1111/epi.13220.Peer-Reviewed Original ResearchConceptsFocal limbic seizuresLimbic seizuresCortical functionBrainstem neuronsCortical dysfunctionFocal temporal lobe seizuresOptogenetic stimulationCortical slow-wave activityDepressed cortical functionSubcortical cholinergic neuronsTemporal lobe seizuresLoss of consciousnessPedunculopontine tegmental nucleusAnesthetized rat modelSlow wave activityCortical gamma activitySleep-like stateCholinergic neuronsFocal seizuresArousal networkCholinergic stimulationTegmental nucleusRat modelCortical physiologySeizuresArousal and Locomotion Make Distinct Contributions to Cortical Activity Patterns and Visual Encoding
Vinck M, Batista-Brito R, Knoblich U, Cardin JA. Arousal and Locomotion Make Distinct Contributions to Cortical Activity Patterns and Visual Encoding. Neuron 2015, 86: 740-754. PMID: 25892300, PMCID: PMC4425590, DOI: 10.1016/j.neuron.2015.03.028.Peer-Reviewed Original ResearchConceptsSensory-evoked cortical activityCortical activity patternsLocomotion effectsMouse V1Spontaneous firingLocal field potentialsCortical circuitsCortical activityRunning wheelArea V1Visual responsesPatterns of activityPupil diameterPopulation activityField potentialsMotor feedbackArousalV1Activity patternsArousal levelTemporal patterningComplementary roleMiceActivity
2011
Dissecting local circuits in vivo: Integrated optogenetic and electrophysiology approaches for exploring inhibitory regulation of cortical activity
Cardin JA. Dissecting local circuits in vivo: Integrated optogenetic and electrophysiology approaches for exploring inhibitory regulation of cortical activity. Journal Of Physiology-Paris 2011, 106: 104-111. PMID: 21958624, PMCID: PMC3277809, DOI: 10.1016/j.jphysparis.2011.09.005.Peer-Reviewed Original ResearchA critical role for NMDA receptors in parvalbumin interneurons for gamma rhythm induction and behavior
Carlén M, Meletis K, Siegle J, Cardin J, Futai K, Vierling-Claassen D, Rühlmann C, Jones S, Deisseroth K, Sheng M, Moore C, Tsai L. A critical role for NMDA receptors in parvalbumin interneurons for gamma rhythm induction and behavior. Molecular Psychiatry 2011, 17: 537-548. PMID: 21468034, PMCID: PMC3335079, DOI: 10.1038/mp.2011.31.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAssociation LearningBrain WavesConditioning, PsychologicalExcitatory Postsynaptic PotentialsGABA AntagonistsGABAergic NeuronsInterneuronsMaleMaze LearningMemory, Short-TermMiceMice, TransgenicParvalbuminsPhotic StimulationPicrotoxinReceptors, N-Methyl-D-AspartateSensory GatingStereotyped BehaviorConceptsGamma oscillationsSpecific cognitive behaviorSelective cognitive impairmentsGamma rhythmsPsychosis-like symptomsCognitive tasksAssociative learningNeural network dysfunctionCognitive behaviorCortical gamma rhythmsCognitive impairmentNetwork dysfunctionNMDAR neurotransmissionOptogenetic drivePV interneuronsParvalbumin interneuronsNMDAR antagonistsMemoryReduced sensitivityBehaviorTaskHabituationDeficitsNormal behaviorLearning
2010
Cellular Mechanisms of Temporal Sensitivity in Visual Cortex Neurons
Cardin JA, Kumbhani RD, Contreras D, Palmer LA. Cellular Mechanisms of Temporal Sensitivity in Visual Cortex Neurons. Journal Of Neuroscience 2010, 30: 3652-3662. PMID: 20219999, PMCID: PMC2880457, DOI: 10.1523/jneurosci.5279-09.2010.Peer-Reviewed Original ResearchConceptsLayer 4 neuronsCortical neuronsSensory-evoked synaptic inputsVisual cortex neuronsCellular mechanismsPrimary visual cortexCortex neuronsInhibitory interneuronsCortical functionExcitatory neuronsSynaptic inputsMembrane potential responsesSpike precisionVisual cortexCortical layersExcitatory conductanceNeuronsCoincident inputsInput integrationSynchronous inputsCortexNonlinear summationTemporal sensitivityAcuityInterneuronsTargeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2
Cardin JA, Carlén M, Meletis K, Knoblich U, Zhang F, Deisseroth K, Tsai LH, Moore CI. Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. Nature Protocols 2010, 5: 247-254. PMID: 20134425, PMCID: PMC3655719, DOI: 10.1038/nprot.2009.228.Peer-Reviewed Original ResearchConceptsOptical interferenceMajor long-term goalViral vectorsCell-type selectivityRecording of neuronsOptogenetic stimulationChannelrhodopsin-2Cre-dependent expressionBrain circuit functionSelective cell typesInhibitory interneuronsIntracellular recordingsVivo electrophysiologyExcitatory neuronsIntact brainType selectivityNeural subtypesOptogenetic techniquesSpecific populationsNeural activityCircuit functionNeuronsInterferenceCell typesStimulation
2009
Driving fast-spiking cells induces gamma rhythm and controls sensory responses
Cardin JA, Carlén M, Meletis K, Knoblich U, Zhang F, Deisseroth K, Tsai LH, Moore CI. Driving fast-spiking cells induces gamma rhythm and controls sensory responses. Nature 2009, 459: 663-667. PMID: 19396156, PMCID: PMC3655711, DOI: 10.1038/nature08002.Peer-Reviewed Original ResearchPinacidil induces vascular dilation and hyperemia in vivo and does not impact biophysical properties of neurons and astrocytes in vitro
Cao R, Higashikubo B, Cardin J, Knoblich U, Ramos R, Nelson M, Moore C, Brumberg J. Pinacidil induces vascular dilation and hyperemia in vivo and does not impact biophysical properties of neurons and astrocytes in vitro. Cleveland Clinic Journal Of Medicine 2009, 76: s80-s85. PMID: 19380306, PMCID: PMC4406396, DOI: 10.3949/ccjm.76.s2.16.Peer-Reviewed Original ResearchConceptsBlood volumeApplication of pinacidilWhole-cell recordingsLocal blood volumeFunctional hyperemiaVascular dilationNeocortical slicesAnesthetized ratsAwake miceSmooth muscleSelective agonistPinacidilImpact of hemodynamicsIntrinsic modulatorHyperemiaNeural activityVasodilationNeuronsVivoBiophysical propertiesNeural systemsHemodynamicsAgonistsNeocortexRats
2008
Cellular Mechanisms Underlying Stimulus-Dependent Gain Modulation in Primary Visual Cortex Neurons In Vivo
Cardin JA, Palmer LA, Contreras D. Cellular Mechanisms Underlying Stimulus-Dependent Gain Modulation in Primary Visual Cortex Neurons In Vivo. Neuron 2008, 59: 150-160. PMID: 18614036, PMCID: PMC2504695, DOI: 10.1016/j.neuron.2008.05.002.Peer-Reviewed Original ResearchConceptsSynaptic activationPrimary visual cortex neuronsCat primary visual cortexVisual cortex neuronsCellular mechanismsPrimary visual cortexNeuronal gainBackground synaptic activityVisual stimuliPostsynaptic effectsCortex neuronsIntracellular recordingsSynaptic activitySynaptic inputsVisual cortexInput resistanceVisual stimulationGain modulationSustained changesVivoSensory contextMembrane potentialNeuronal phenomenaActivationModulation
2007
Stimulus Feature Selectivity in Excitatory and Inhibitory Neurons in Primary Visual Cortex
Cardin JA, Palmer LA, Contreras D. Stimulus Feature Selectivity in Excitatory and Inhibitory Neurons in Primary Visual Cortex. Journal Of Neuroscience 2007, 27: 10333-10344. PMID: 17898205, PMCID: PMC3025280, DOI: 10.1523/jneurosci.1692-07.2007.Peer-Reviewed Original ResearchConceptsPrimary visual cortexFS cellsVisual cortexRS cellsLayer 4Feature selectivityThalamorecipient layer 4Cortical sensory processingResponses of excitatorySynaptic responsesInhibitory neuronsSynaptic inputsSynaptic integrationInhibitory cellsMembrane potential fluctuationsStimulus selectivitySpike outputLow input resistanceFiring rateSpike tuningType-specific differencesInput resistanceSpiking cellsCell type-specific differencesRegular spiking
1999
Memory suppressor genes: Enhancing the relationship between synaptic plasticity and memory storage
Cardin J, Abel T. Memory suppressor genes: Enhancing the relationship between synaptic plasticity and memory storage. Journal Of Neuroscience Research 1999, 58: 10-23. PMID: 10491568, DOI: 10.1002/(sici)1097-4547(19991001)58:1<10::aid-jnr3>3.0.co;2-2.Peer-Reviewed Original ResearchConceptsMemory suppressor genesSuppressor gene productGene productsSuppressor geneTranscriptional regulatory proteinsCell-cell interactionsIntracellular signaling cascadesSynaptic plasticityTranscriptional controlRegulatory proteinsSignaling cascadesKinase activityHuman disordersGenesInhibitory constraintCell surfaceFuture pharmaceuticalsLong-term potentiationProteinPotential targetMammalian hippocampusPlasticityLong-term facilitationRecent evidenceDrosophila