Featured Publications
The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12
Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee R, Mootha VK. The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12. Cell 2017, 171: 771-782.e11. PMID: 29056341, PMCID: PMC5827971, DOI: 10.1016/j.cell.2017.09.051.Peer-Reviewed Original ResearchMeSH KeywordsCarbon-Carbon LyasesGene Knockout TechniquesHumansMetabolic Networks and PathwaysMitochondriaModels, MolecularSuccinatesVitamin B 12ConceptsMethylmalonyl-CoA mutaseCitramalyl-CoA lyaseAnti-microbial metabolitesCell-autonomous defectSubstrate analogue inhibitorsMammalian cellsStructural biologyPathway assignmentItaconyl-CoAMitochondrial enzymesCellular activitiesMetabolic pathwaysDependent methylmalonyl-CoA mutaseHomozygous lossConsistent phenotypeConsequence of exposureVertebratesCellsEnzymologyBiologyMutaseLyasePhenotypeEnzymePathway
2013
Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors
Park RJ, Shen H, Liu L, Liu X, Ferguson SM, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. Journal Of Cell Science 2013, 126: 5305-5312. PMID: 24046449, PMCID: PMC3828596, DOI: 10.1242/jcs.138578.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDynamin IDynamin IIDynamin IIIEndocytosisFibroblastsGene Expression RegulationGene Knockout TechniquesHydrazonesMiceNaphtholsSynapsesConceptsDynamin 1Dynamin inhibitorTKO cellsPeripheral membrane rufflingDouble knockoutDynamin 3 geneClathrin-mediated endocytosisTriple-knockout cellsDynamin-dependent processFluid-phase endocytosisDyngo-4aMembrane fissionMembrane rufflingDKO cellsDynamin 2Knockout cellsLow-level expressionCell physiologyDynaminEndocytosisTarget effectsDKO fibroblastsGenesCellsKO fibroblasts
2010
Constitutive activated Cdc42-associated kinase (Ack) phosphorylation at arrested endocytic clathrin-coated pits of cells that lack dynamin
Shen H, Ferguson SM, Dephoure N, Park R, Yang Y, Volpicelli-Daley L, Gygi S, Schlessinger J, De Camilli P. Constitutive activated Cdc42-associated kinase (Ack) phosphorylation at arrested endocytic clathrin-coated pits of cells that lack dynamin. Molecular Biology Of The Cell 2010, 22: 493-502. PMID: 21169560, PMCID: PMC3038647, DOI: 10.1091/mbc.e10-07-0637.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCdc42 GTP-Binding ProteinCell LineChlorocebus aethiopsClathrinClathrin Heavy ChainsClathrin Light ChainsCoated Pits, Cell-MembraneCOS CellsDynamin IDynamin IIEndocytosisFibroblastsGene Knockout TechniquesHumansMiceMice, KnockoutPhosphorylationProtein BindingProtein-Tyrosine KinasesSignal TransductionConceptsEndocytic clathrin-coated pitsClathrin-coated pitsRNA interference-based approachesFundamental cellular processesGrowth factor receptor signalingWild-type cellsNonreceptor tyrosine kinaseActive Cdc42Cellular processesSpecific cargoClathrin assemblyEndocytic routeEndocytic vesiclesGrowth factor receptorTyrosine phosphorylationPhosphorylation stateClathrin boxTyrosine kinaseKinase phosphorylationCdc42Receptor signalingActivation-deactivation cyclePhosphorylationDynaminFactor receptor
2009
Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits
Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, Ferguson A, Destaing O, Ko G, Takasaki J, Cremona O, Toole E, De Camilli P. Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits. Developmental Cell 2009, 17: 811-822. PMID: 20059951, PMCID: PMC2861561, DOI: 10.1016/j.devcel.2009.11.005.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell MembraneClathrinCoated Pits, Cell-MembraneCytoskeletonDynamin IDynamin IIEndocytosisGene Knockout TechniquesMiceMice, KnockoutConceptsEndocytic roleEndocytic clathrin-coated pitsBAR domain proteinsSense membrane curvatureClathrin-coated pitsWild-type cellsGTPase dynaminEndocytic intermediatesHigher eukaryotesDomain proteinsMembrane fissionActin dynamicsNumerous proteinsDynaminMembrane curvatureProtein upstreamTubular neckConcerted actionProteinActinCoordinated actionKey playersFunctional relationshipCellsSequence of events