Featured Publications
The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12
Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee R, Mootha VK. The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12. Cell 2017, 171: 771-782.e11. PMID: 29056341, PMCID: PMC5827971, DOI: 10.1016/j.cell.2017.09.051.Peer-Reviewed Original ResearchConceptsMethylmalonyl-CoA mutaseCitramalyl-CoA lyaseAnti-microbial metabolitesCell-autonomous defectSubstrate analogue inhibitorsMammalian cellsStructural biologyPathway assignmentItaconyl-CoAMitochondrial enzymesCellular activitiesMetabolic pathwaysDependent methylmalonyl-CoA mutaseHomozygous lossConsistent phenotypeConsequence of exposureVertebratesCellsEnzymologyBiologyMutaseLyasePhenotypeEnzymePathway
2019
Itaconyl-CoA forms a stable biradical in methylmalonyl-CoA mutase and derails its activity and repair
Ruetz M, Campanello GC, Purchal M, Shen H, McDevitt L, Gouda H, Wakabayashi S, Zhu J, Rubin EJ, Warncke K, Mootha VK, Koutmos M, Banerjee R. Itaconyl-CoA forms a stable biradical in methylmalonyl-CoA mutase and derails its activity and repair. Science 2019, 366: 589-593. PMID: 31672889, PMCID: PMC7070230, DOI: 10.1126/science.aay0934.Peer-Reviewed Original ResearchMeSH KeywordsBiocatalysisCatalytic DomainCoenzyme ACrystallography, X-RayDeoxyadenosinesElectron Spin Resonance SpectroscopyHumansHydrogen BondingMacrophagesMethylmalonyl-CoA MutaseModels, MolecularMycobacterium tuberculosisPropionatesProtein ConformationProtein MultimerizationProtein SubunitsSuccinatesVitamin B 12