Featured Publications
The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12
Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee R, Mootha VK. The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12. Cell 2017, 171: 771-782.e11. PMID: 29056341, PMCID: PMC5827971, DOI: 10.1016/j.cell.2017.09.051.Peer-Reviewed Original ResearchConceptsMethylmalonyl-CoA mutaseCitramalyl-CoA lyaseAnti-microbial metabolitesCell-autonomous defectSubstrate analogue inhibitorsMammalian cellsStructural biologyPathway assignmentItaconyl-CoAMitochondrial enzymesCellular activitiesMetabolic pathwaysDependent methylmalonyl-CoA mutaseHomozygous lossConsistent phenotypeConsequence of exposureVertebratesCellsEnzymologyBiologyMutaseLyasePhenotypeEnzymePathway
2019
Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation
Wang LW, Shen H, Nobre L, Ersing I, Paulo JA, Trudeau S, Wang Z, Smith NA, Ma Y, Reinstadler B, Nomburg J, Sommermann T, Cahir-McFarland E, Gygi SP, Mootha VK, Weekes MP, Gewurz BE. Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation. Cell Metabolism 2019, 30: 539-555.e11. PMID: 31257153, PMCID: PMC6720460, DOI: 10.1016/j.cmet.2019.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAminohydrolasesB-LymphocytesCell Transformation, ViralEpstein-Barr Virus InfectionsEpstein-Barr Virus Nuclear AntigensFemaleFolic AcidGlycolysisHEK293 CellsHerpesvirus 4, HumanHumansLymphocyte ActivationMethylenetetrahydrofolate Dehydrogenase (NADP)MitochondriaMultifunctional EnzymesNADPOxidation-ReductionProteomeProto-Oncogene Proteins c-mycSerineConceptsEpstein-Barr virusOne-carbon metabolismSynthesis of serinePost-transplant B cell lymphomasB-cell transformationPrimary human B cellsMitochondrial remodelingTargets MYCRedox defenseNADPH productionB-cell outgrowthCell transformationMetabolic pathwaysB-cell lymphomaPotential therapeutic targetB cell growthMitochondrial NADPHViral proteinsB cell proliferationCell growthNADPH levelsHuman B cellsCell proliferationMTHFD2Infected cells