2016
Leishmania‐encoded orthologs of macrophage migration inhibitory factor regulate host immunity to promote parasite persistence
Holowka T, Castilho TM, Garcia AB, Sun T, McMahon‐Pratt D, Bucala R. Leishmania‐encoded orthologs of macrophage migration inhibitory factor regulate host immunity to promote parasite persistence. The FASEB Journal 2016, 30: 2249-2265. PMID: 26956417, PMCID: PMC4871794, DOI: 10.1096/fj.201500189r.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Differentiation, B-LymphocyteApoptosisCD4-Positive T-LymphocytesCloning, MolecularGene DeletionGene Expression RegulationHistocompatibility Antigens Class IILeishmania majorLeishmaniasis, CutaneousMacrophage Migration-Inhibitory FactorsMacrophagesMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutMice, SCIDOrganisms, Genetically ModifiedProtein Array AnalysisProtozoan ProteinsConceptsMacrophage migration inhibitory factorMigration inhibitory factorCD4 T cellsInhibitory factorL. majorT cellsHost immunityProtective CD4 T cellsEffector CD4 T cellsCytokine macrophage migration inhibitory factorMajor-infected miceT cell primingAntigen-presenting cellsT cell formationExpression of IFNDeath-1Functional exhaustionIL-7RHost responseParasite persistenceParasite burdenParasite growthReduced expressionMiceSignificant differences
2011
TLR1/2 Activation during Heterologous Prime-Boost Vaccination (DNA-MVA) Enhances CD8+ T Cell Responses Providing Protection against Leishmania (Viannia)
Jayakumar A, Castilho TM, Park E, Goldsmith-Pestana K, Blackwell JM, McMahon-Pratt D. TLR1/2 Activation during Heterologous Prime-Boost Vaccination (DNA-MVA) Enhances CD8+ T Cell Responses Providing Protection against Leishmania (Viannia). PLOS Neglected Tropical Diseases 2011, 5: e1204. PMID: 21695103, PMCID: PMC3114751, DOI: 10.1371/journal.pntd.0001204.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesDisease Models, AnimalFemaleGenetic VectorsImmunization, SecondaryInterferon-gammaInterleukin-10Interleukin-13LeishmaniaLeishmaniasisLeishmaniasis VaccinesMiceMice, Inbred BALB CPeroxidasesProtozoan ProteinsRodent DiseasesToll-Like Receptor 1Toll-Like Receptor 2VaccinationVaccines, DNAVaccines, SyntheticVaccinia virusViral VaccinesConceptsPrime-boost vaccinationHeterologous prime-boost vaccinationCD8 T cellsT cell responsesT cellsTLR1/2 activationIL-10Vaccination modalityIL-13Immune responseAntigen-specific CD8 cellsCD8 T cell responsesCell responsesL. panamensis infectionsSpecific CD8 cellsTLR1/2 agonist Pam3CSK4IL-10 responsesVaccine-induced protectionCD4 T cellsMurine immune responseIL-13 responsesLeishmania speciesInfection/diseaseVaccinia virus AnkaraInnate immune response
2008
Intradermal NKT cell activation during DNA priming in heterologous prime‐boost vaccination enhances T cell responses and protection against Leishmania
Dondji B, Deak E, Goldsmith‐Pestana K, Perez‐Jimenez E, Esteban M, Miyake S, Yamamura T, McMahon‐Pratt D. Intradermal NKT cell activation during DNA priming in heterologous prime‐boost vaccination enhances T cell responses and protection against Leishmania. European Journal Of Immunology 2008, 38: 706-719. PMID: 18286565, PMCID: PMC3448375, DOI: 10.1002/eji.200737660.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationAntigens, ProtozoanCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesGalactosylceramidesGenetic VectorsGranzymesImmunity, CellularInterferon-gammaInterleukin-10Killer Cells, NaturalLeishmaniasisLymphocyte ActivationLymphocyte DepletionMiceMice, Inbred BALB CMice, Mutant StrainsNitric OxideProtozoan ProteinsSkinT-LymphocytesVaccinationVaccines, DNAVaccinia virusConceptsHeterologous prime-boost vaccinationPrime-boost vaccinationNKT cell activationCD8 T cellsT cellsCell activationVaccinated miceDNA primingActivated C-kinase (rLACK) antigensT cell immune responsesDevelopment of CD4Murine cutaneous leishmaniasisT cell responsesCell immune responsesElicit protective immunityIL-10Protective immunityImmune responseLeishmania homologueIFN-gammaAlphaGalCerCutaneous leishmaniasisVisceral leishmaniasisParasite burdenCell responses
2002
Heterogeneity, geographic distribution, and pathogenicity of serodemes of Leishmania viannia in Colombia.
Saravia NG, Weigle K, Navas C, Segura I, Valderrama L, Valencia AZ, Escorcia B, McMahon-Pratt D. Heterogeneity, geographic distribution, and pathogenicity of serodemes of Leishmania viannia in Colombia. American Journal Of Tropical Medicine And Hygiene 2002, 66: 738-44. PMID: 12224583, DOI: 10.4269/ajtmh.2002.66.738.Peer-Reviewed Original Research
2001
Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8
Colmenares M, Tiemeyer M, Kima P, McMahon-Pratt D. Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8. Infection And Immunity 2001, 69: 6776-6784. PMID: 11598050, PMCID: PMC100055, DOI: 10.1128/iai.69.11.6776-6784.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ProtozoanFemaleGlycolipidsLeishmaniaMembrane ProteinsMiceMice, Inbred BALB CProteolipidsProtozoan ProteinsProtozoan VaccinesConceptsAntigen PFurther vaccine studiesSignificant T cell activationT cell activationWestern blot analysisProtective immunityVaccine studiesApolipoprotein EMurine modelCutaneous leishmaniasisSignificant protectionComparable protectionImmunodominant componentAntigenic glycolipidsImmunity studiesAntigenCysteine proteinasesGlycolipid componentBiochemical analysisBiological characterizationSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisInfectionLeishmaniasis
1992
Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex
McMahon-Pratt D, Traub-Cseko Y, Lohman K, Rogers D, Beverley S. Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex. Molecular And Biochemical Parasitology 1992, 50: 151-160. PMID: 1542309, DOI: 10.1016/0166-6851(92)90252-f.Peer-Reviewed Original ResearchConceptsGP46/MGene familyGlycoprotein gene familyMolecular karyotype analysisBraziliensis complexLeishmania braziliensis complexSpeciation eventsTrypanosomatid speciesPhylogenetic analysisCrithidia fasciculataLeishmania tarentolaeDevelopmental cycleWestern blot analysisLeishmania mexicana complexMembrane glycoproteinsKaryotype analysisBlot analysisL. braziliensis complexProtein expressionPolyclonal antiserumMexicana complexSpeciesL. panamensisLeishmania majorL. braziliensis
1991
Biochemical characterization of the protective membrane glycoprotein GP46/M-2 of Leishmania amazonensis
Rivas L, Kahl L, Manson K, McMahon-Pratt D. Biochemical characterization of the protective membrane glycoprotein GP46/M-2 of Leishmania amazonensis. Molecular And Biochemical Parasitology 1991, 47: 235-243. PMID: 1719417, DOI: 10.1016/0166-6851(91)90183-7.Peer-Reviewed Original ResearchConceptsGP46/MCarbohydrate side chainsDNA-derived protein sequenceLipid anchorSpecies-specific epitopesProtein sequencesSide chainsBiochemical characterizationLeishmania amazonensisProteinBiochemical featuresPolypeptide portionSurface membraneMonoclonal antibodiesPolypeptideAmazonensisMoleculesEpitopesAnchorsSequenceMembraneDigestionAntibodiesChainPromastigotes
1990
Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.
LeBowitz J, Coburn C, McMahon-Pratt D, Beverley S. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 9736-9740. PMID: 2124701, PMCID: PMC55248, DOI: 10.1073/pnas.87.24.9736.Peer-Reviewed Original ResearchConceptsExpression vectorCis-acting elementsTrypanosomatid protozoan parasitesRNA editingSurface antigen geneMolecular paradigmProcessing eventsParasite proteinsGenetic analysisLeishmania expression vectorExpression sitesPathogenic speciesNormal transcriptMembrane glycoproteinsEscherichia coliGenesImmunoblot analysisProtozoan parasiteProteinAntigen geneColony assayLeishmania majorFamily membersL. amazonensisKilobasesMolecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.
Lohman K, Langer P, McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8393-8397. PMID: 2236047, PMCID: PMC54962, DOI: 10.1073/pnas.87.21.8393.Peer-Reviewed Original ResearchConceptsGP46/MDNA sequence dataHydrophobic leader peptideCarboxyl-terminal domainMetal-binding motifGlycosyl-phosphatidylinositol anchorAmino-terminal portionProtozoan Leishmania amazonensisSouthern blot analysisGene familyMature proteinPutative attachment siteMolecular cloningProtein comprisesRepetitive sequencesSequence dataCarboxyl terminusCysteine residuesProtein sequencesLeader peptideMembrane locationBinding motifLeishmania amazonensisMembrane glycoproteinsBlot analysisProphylactic Immunization against Experimental Leishmania donovani Infection by Use of a Purified Protein Vaccine
White A, McMahon-Pratt D. Prophylactic Immunization against Experimental Leishmania donovani Infection by Use of a Purified Protein Vaccine. The Journal Of Infectious Diseases 1990, 161: 1313-1314. PMID: 2345310, DOI: 10.1093/infdis/161.6.1313.Peer-Reviewed Original Research
1989
Flagellar Membrane and Paraxial Rod Proteins of Leishmania: Characterization Employing Monoclonal Antibodies
ISMACH R, CIANCI C, CAULFIELD J, LANGER P, HEIN A, McMAHON‐PRATT D. Flagellar Membrane and Paraxial Rod Proteins of Leishmania: Characterization Employing Monoclonal Antibodies. Journal Of Eukaryotic Microbiology 1989, 36: 617-624. PMID: 2689639, DOI: 10.1111/j.1550-7408.1989.tb01105.x.Peer-Reviewed Original ResearchConceptsDoublet of proteinsImmunocytochemical electron microscopic studyIndirect immunofluorescence experimentsRod proteinsMonoclonal antibody fFlagellar membraneSingle proteinDifferent proteinsFlagellar tipImmunofluorescence experimentsFlagellaParaxial rodProteinMonoclonal antibodiesAntibody FElectron microscopic experimentsMembraneLeishmaniaElectron microscopic studyMoleculesDAMicroscopic experimentsMicroscopic studyMonoclonal antibody techniqueLocalized patches