2023
Injury prevents Ras mutant cell expansion in mosaic skin
Gallini S, Annusver K, Rahman N, Gonzalez D, Yun S, Matte-Martone C, Xin T, Lathrop E, Suozzi K, Kasper M, Greco V. Injury prevents Ras mutant cell expansion in mosaic skin. Nature 2023, 619: 167-175. PMID: 37344586, PMCID: PMC10322723, DOI: 10.1038/s41586-023-06198-y.Peer-Reviewed Original ResearchConceptsWild-type cellsRas family proteinsCell cycle inhibitor p21Family proteinsOncogenic RasGenetic approachesMosaic tissuesInhibition of EGFRInhibitor p21EGFR ligandsEGFR pathwayCell expansionAberrant growthConstitutive lossDifferential activationParacrine secretionAbsence of injuryCellsCompetitive balanceInjury repairHealthy skinInjurySkinProteinPathwayLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2022
Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer
Cockburn K, Annusver K, Gonzalez D, Ganesan S, May D, Mesa K, Kawaguchi K, Kasper M, Greco V. Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer. Nature Cell Biology 2022, 24: 1692-1700. PMID: 36357619, PMCID: PMC9729105, DOI: 10.1038/s41556-022-01021-8.Peer-Reviewed Original ResearchConceptsCell cycle exitCycle exitStem cell layerUndifferentiated stem cellsStem cell compartmentDaughter cellsDifferentiated populationsDifferentiation genesTranscriptional changesCell divisionMultiple progenitorsStemness genesCells transitStem cellsCell compartmentCell layerCell poolDifferentiationRegenerative tissueGenesSkin epidermisGradual differentiationMulti-day processesCells
2021
Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis
Park S, Matte-Martone C, Gonzalez DG, Lathrop EA, May DP, Pineda CM, Moore JL, Boucher JD, Marsh E, Schmitter-Sánchez A, Cockburn K, Markova O, Bellaïche Y, Greco V. Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis. Nature Cell Biology 2021, 23: 476-484. PMID: 33958758, PMCID: PMC8603572, DOI: 10.1038/s41556-021-00670-5.Peer-Reviewed Original ResearchConceptsDendritic epidermal T cellsLangerhans cellsCell typesEpithelial cellsDifferent cell typesMultiple cell typesLive adult miceGTPase Rac1Skin-resident immune cellsNon-random spatial distributionDistribution of LCContinuous turnoverEpidermal cellsEpidermal T cellsIndividual cellsCellular mechanismsEnvironmental insultsHomeostasisBasal epithelial cellsImmune cellsT cellsAdult miceCellsProper architectureEpidermis
2019
Hair follicle regeneration suppresses Ras-driven oncogenic growth
Pineda CM, Gonzalez DG, Matte-Martone C, Boucher J, Lathrop E, Gallini S, Fons NR, Xin T, Tai K, Marsh E, Nguyen DX, Suozzi KC, Beronja S, Greco V. Hair follicle regeneration suppresses Ras-driven oncogenic growth. Journal Of Cell Biology 2019, 218: 3212-3222. PMID: 31488583, PMCID: PMC6781447, DOI: 10.1083/jcb.201907178.Peer-Reviewed Original ResearchConceptsHair folliclesHras mutationsOncogenic growthHair follicle stem cellsSkin hair folliclesTumor developmentFollicle stem cellsHair follicle regenerationSkin epitheliumSecondary mutationsBenign outgrowthFolliclesStem cellsTissueCertain tissuesFollicle regenerationCellsContinuous tissueWild-type neighborsDistinct mechanismsDifferent outcomesMutationsEnhanced capacityInjury
2018
Flexible fate determination ensures robust differentiation in the hair follicle
Xin T, Gonzalez D, Rompolas P, Greco V. Flexible fate determination ensures robust differentiation in the hair follicle. Nature Cell Biology 2018, 20: 1361-1369. PMID: 30420661, PMCID: PMC6314017, DOI: 10.1038/s41556-018-0232-y.Peer-Reviewed Original ResearchConceptsSingle-cell levelStem cellsStem cell differentiationGerm stem cellsTissue architectureMultiple cell typesFate determinationDetermination mechanismTissue homeostasisSame stem cellsCommon progenitorDifferentiation outcomesDifferentiation stimuliDifferentiation lineageCell differentiationCell typesNormal differentiationWnt activationHair folliclesUnanticipated flexibilityDifferentiationRobust differentiationProgenitorsCellsUninjured condition
2017
Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice
Park S, Gonzalez DG, Guirao B, Boucher JD, Cockburn K, Marsh ED, Mesa KR, Brown S, Rompolas P, Haberman AM, Bellaïche Y, Greco V. Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice. Nature Cell Biology 2017, 19: 155-163. PMID: 28248302, PMCID: PMC5581297, DOI: 10.1038/ncb3472.Peer-Reviewed Original ResearchTissue-scale organizationEpidermal wound repairRegional coexistenceCellular behaviorDifferentiated cellsEpithelial behaviorUndamaged cellsProliferation functionTissue architectureHomeostatic functionsDifferentiation changesLive mammalsLive miceWound repairTissue repairProliferative zoneHomeostatic behaviorCellsSkin repairSpatiotemporal dynamicsMammalsWoundsMiceRepairMigration