2022
Redox regulation of RAD51 Cys319 and homologous recombination by peroxiredoxin 1
Skoko J, Cao J, Gaboriau D, Attar M, Asan A, Hong L, Paulsen C, Ma H, Liu Y, Wu H, Harkness T, Furdui C, Manevich Y, Morrison C, Brown E, Normolle D, Spies M, Spies M, Carroll K, Neumann C. Redox regulation of RAD51 Cys319 and homologous recombination by peroxiredoxin 1. Redox Biology 2022, 56: 102443. PMID: 36058112, PMCID: PMC9450138, DOI: 10.1016/j.redox.2022.102443.Peer-Reviewed Original ResearchConceptsRAD51 foci formationFoci formationRedox regulationHomologous recombination DNA repair pathwayHR DNA repairProtein binding partnersPoly (ADP-ribose) polymeraseDNA repair pathwaysDAz-2Rad51 proteinSensitization of cellsRad51 filamentsBinding partnerHomologous recombinationDNA repairCysteine oxidationRepair pathwaysMediator proteinsHuman breast cancer cellsCellular responsesPeroxiredoxin 1Breast cancer cellsDNA damagePRDX1Cys319
2009
Orchestrating Redox Signaling Networks through Regulatory Cysteine Switches
Paulsen CE, Carroll KS. Orchestrating Redox Signaling Networks through Regulatory Cysteine Switches. ACS Chemical Biology 2009, 5: 47-62. PMID: 19957967, PMCID: PMC4537063, DOI: 10.1021/cb900258z.Peer-Reviewed Original ResearchConceptsProtein functionReactive oxygen speciesIntracellular signal transductionCysteine switchRedox regulationSignal transductionCysteine oxidationCysteine residuesCurrent mechanistic insightsSecond messengerDiverse mechanismsMechanistic understandingMechanistic insightsOxygen speciesChemoselective oxidationNew therapeutic strategiesHydrogen peroxideRecent advancesOxidationTherapeutic strategiesTransductionSpeciesProteinMessengerResiduesChemical Dissection of an Essential Redox Switch in Yeast
Paulsen CE, Carroll KS. Chemical Dissection of an Essential Redox Switch in Yeast. Cell Chemical Biology 2009, 16: 217-225. PMID: 19230722, DOI: 10.1016/j.chembiol.2009.01.003.Peer-Reviewed Original ResearchConceptsSulfenic acidCell-permeable chemical probesSulfenic acid modificationCysteine sulfenic acid modificationTranscription factor Yap1Chemical probesCysteine oxidationRedox switchElectrostatic calculationsIntermolecular disulfide formationCharge distributionHydrogen peroxideAcid modificationNuclear accumulationIntermolecular disulfideConformational rearrangementsDisulfide formationCentral unresolved questionYAP1 activationYAP1GPX3Chemical dissectionCatalysisUnresolved questionsAcid