A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries
Barrett A, McWhirter R, Taylor SR, Weinreb A, Miller DM, Hammarlund M. A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries. G3: Genes, Genomes, Genetics 2021, 11: jkab121. PMID: 33856427, PMCID: PMC8495925, DOI: 10.1093/g3journal/jkab121.Peer-Reviewed Original ResearchConceptsC. elegans RNANoisy gene expressionRRNA gene sequencesRNA sequencing librariesRare cell populationsSuch challenging samplesCaenorhabditis elegansLong genesRibosomal RNAGene sequencesNoncoding RNAsCustom kitRNAseq librariesGene expressionPolyA selectionRRNA depletionLibrary preparationLibrary constructionRNAPCR duplicatesGenesRibodepletionCell populationsCell samplesExpression