Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos.
Yuan S, Sun Z. Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos. Journal Of Visualized Experiments 2009 PMID: 19488022, PMCID: PMC2762915, DOI: 10.3791/1113.Peer-Reviewed Original ResearchConceptsZebrafish embryosOverexpression of EGFPMorpholino oligonucleotide injectionKnockdown of PKD2Microinjection of RNAGerm-line chimerasMicroinjection of mRNACell fate mappingMorpholino microinjectionAutosomal dominant polycystic kidney diseaseGene functionMisexpression studiesMorpholino antisenseMorpholino oligosGene knockdownTransgenic animalsGFP expressionOligonucleotide injectionEGFP mRNAPhenotype analysisEmbryosGene screeningMRNABroad applicationsPKD2