2021
Epigallocatechin gallate facilitates extracellular elastin fiber formation in induced pluripotent stem cell derived vascular smooth muscle cells for tissue engineering
Ellis MW, Riaz M, Huang Y, Anderson CW, Luo J, Park J, Lopez CA, Batty LD, Gibson KH, Qyang Y. Epigallocatechin gallate facilitates extracellular elastin fiber formation in induced pluripotent stem cell derived vascular smooth muscle cells for tissue engineering. Journal Of Molecular And Cellular Cardiology 2021, 163: 167-174. PMID: 34979103, PMCID: PMC8920537, DOI: 10.1016/j.yjmcc.2021.12.014.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsTissue engineeringStem cell derivativesPluripotent stem cell derivativesInduced pluripotent stem cellsStem cellsGraft productionMechanical strengthExtracellular formationExpression systemCell derivativesVascular smooth muscle cellsElastin fiber formationEngineered graftSmooth muscle cellsFiber formationNotable obstacleLack of elastinMuscle cellsEngineeringClinical applicationVascular graftsCell proliferative capacityElastin productionProliferative capacityMethods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Li ML, Luo J, Ellis MW, Riaz M, Ajaj Y, Qyang Y. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells. Methods In Molecular Biology 2021, 2375: 21-34. PMID: 34591296, DOI: 10.1007/978-1-0716-1708-3_3.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsVascular smooth muscle cellsPluripotent stem cellsLateral plate mesodermEarly embryonic developmentStem cellsSmooth muscle cellsHuman pluripotent stem cellsInduced pluripotent stem cellsExtracellular matrix proteinsMuscle cellsMesoderm lineagePlate mesodermEmbryonic developmentVascular cell sourceEmbryoid bodiesEB formationMatrix proteinsCellular interactionsDisease modelingPhysiological characteristicsVascular tissueTissue-engineered vascular graftsCell-based therapiesCell replacement
2020
Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering
Luo J, Lin Y, Shi X, Li G, Kural MH, Anderson CW, Ellis MW, Riaz M, Tellides G, Niklason LE, Qyang Y. Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2020, 119: 155-168. PMID: 33130306, PMCID: PMC8168373, DOI: 10.1016/j.actbio.2020.10.042.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringTissue-engineered vascular graftsTissue engineeringComparable mechanical strengthVascular smooth muscle cellsMechanical strengthSmooth muscle cellsPolyglycolic acid scaffoldsTechnology one stepBiodegradable polyglycolic acid (PGA) scaffoldsXenogeneic-free conditionsAnimal-derived reagentsMuscle cellsVSMC differentiationImmunodeficient mouse modelEngineeringVascular graftsOne-stepStem cellsPluripotent stem cellsMouse modelCollagen depositionComparable capacityBlood vesselsAcid scaffolds
2018
Modeling elastin-associated vasculopathy with patient induced pluripotent stem cells and tissue engineering
Ellis MW, Luo J, Qyang Y. Modeling elastin-associated vasculopathy with patient induced pluripotent stem cells and tissue engineering. Cellular And Molecular Life Sciences 2018, 76: 893-901. PMID: 30460472, PMCID: PMC6433159, DOI: 10.1007/s00018-018-2969-7.Peer-Reviewed Original ResearchConceptsExtracellular matrix protein elastinAberrant vascular smooth muscle cell (VSMC) proliferationPatient induced pluripotent stem cellsInduced pluripotent stem cellsHuman disease modelingPluripotent stem cell (iPSC) technologyPluripotent stem cellsDrug screening approachesVascular proliferative diseasesVascular smooth muscle cell proliferationStem cell technologyProtein elastinReplenishable supplyDisruption of functionSmooth muscle cell proliferationDisease modelingStem cellsMuscle cell proliferationCell proliferationScreening approachTherapeutic developmentProliferative diseasesElastinBlood vessel dysfunctionTissue engineering
2017
Vascular smooth muscle cells derived from inbred swine induced pluripotent stem cells for vascular tissue engineering
Luo J, Qin L, Kural MH, Schwan J, Li X, Bartulos O, Cong XQ, Ren Y, Gui L, Li G, Ellis MW, Li P, Kotton DN, Dardik A, Pober JS, Tellides G, Rolle M, Campbell S, Hawley RJ, Sachs DH, Niklason LE, Qyang Y. Vascular smooth muscle cells derived from inbred swine induced pluripotent stem cells for vascular tissue engineering. Biomaterials 2017, 147: 116-132. PMID: 28942128, PMCID: PMC5638652, DOI: 10.1016/j.biomaterials.2017.09.019.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsPluripotent stem cellsFunctional vascular smooth muscle cellsMassachusetts General Hospital miniature swineMuscle cellsSelf-assembly approachBiodegradable polyglycolic acid (PGA) scaffoldsPrimary vascular smooth muscle cellsSmooth muscle myosin heavy chainMuscle myosin heavy chainVascular tissue engineeringStem cellsTissue engineeringPolyglycolic acid scaffoldsReprogramming factorsVascular diseaseContractile functionVascular constructsImmunodeficient miceOrgan transplantsMiniature swinePreclinical investigationsGreat potentialMyosin heavy chainEngineered microvasculature in PDMS networks using endothelial cells derived from human induced pluripotent stem cells
Sivarapatna A, Ghaedi M, Xiao Y, Han E, Aryal B, Zhou J, Fernandez-Hernando C, Qyang Y, Hirschi K, Niklason L. Engineered microvasculature in PDMS networks using endothelial cells derived from human induced pluripotent stem cells. Cell Transplantation 2017 DOI: 10.3727/096368916x695236.Peer-Reviewed Original Research
2016
Anisotropic engineered heart tissue made from laser-cut decellularized myocardium
Schwan J, Kwaczala AT, Ryan TJ, Bartulos O, Ren Y, Sewanan LR, Morris AH, Jacoby DL, Qyang Y, Campbell SG. Anisotropic engineered heart tissue made from laser-cut decellularized myocardium. Scientific Reports 2016, 6: 32068. PMID: 27572147, PMCID: PMC5004193, DOI: 10.1038/srep32068.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnisotropyCell Culture TechniquesCells, CulturedEmbryonic Stem CellsInduced Pluripotent Stem CellsLasers, GasMechanotransduction, CellularMyocardial ContractionMyocardiumMyocytes, CardiacPolytetrafluoroethyleneRatsSwineTissue EngineeringTissue ScaffoldsTomography, Optical CoherenceTriiodothyronineTissue-Engineered Vascular Rings from Human iPSC-Derived Smooth Muscle Cells
Dash BC, Levi K, Schwan J, Luo J, Bartulos O, Wu H, Qiu C, Yi T, Ren Y, Campbell S, Rolle MW, Qyang Y. Tissue-Engineered Vascular Rings from Human iPSC-Derived Smooth Muscle Cells. Stem Cell Reports 2016, 7: 19-28. PMID: 27411102, PMCID: PMC4945325, DOI: 10.1016/j.stemcr.2016.05.004.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringFunctional vascular smooth muscle cellsCell-based tissueSelf-assembly approachRenewable sourcesTissue engineeringPluripotent stem cellsPlatform technologyBiomedical applicationsTissue ringsDrug screeningDisease modelingTissue model systemsHuman iPSCStem cellsBroad utilityEfficient approachLarge quantitiesEngineeringMaterialsImplantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraft
2014
Advancements in Induced Pluripotent Stem Cell Technology for Cardiac Regenerative Medicine
Suh CY, Wang Z, Bártulos O, Qyang Y. Advancements in Induced Pluripotent Stem Cell Technology for Cardiac Regenerative Medicine. Journal Of Cardiovascular Pharmacology And Therapeutics 2014, 19: 330-339. PMID: 24651517, PMCID: PMC4169350, DOI: 10.1177/1074248414523676.Peer-Reviewed Original ResearchCardiovascular diseaseCause of morbidityCardiac cellsCardiac regenerative medicineCardiac regenerative therapyPromising cell sourcePluripotent stem cell (iPSC) technologyDiseaseRegenerative therapyInduced pluripotent stem cell (iPSC) technologyCellular typesCell sourceTherapyStem cellsStem cell technologyPluripotent stem cellsRegenerative medicine
2012
Modeling Supravalvular Aortic Stenosis Syndrome With Human Induced Pluripotent Stem Cells
Ge X, Ren Y, Bartulos O, Lee MY, Yue Z, Kim KY, Li W, Amos PJ, Bozkulak EC, Iyer A, Zheng W, Zhao H, Martin KA, Kotton DN, Tellides G, Park IH, Yue L, Qyang Y. Modeling Supravalvular Aortic Stenosis Syndrome With Human Induced Pluripotent Stem Cells. Circulation 2012, 126: 1695-1704. PMID: 22914687, PMCID: PMC3586776, DOI: 10.1161/circulationaha.112.116996.Peer-Reviewed Original ResearchConceptsActin filament bundlesSmooth muscle αSmooth muscle cellsExtracellular signal-regulated kinase 1/2Muscle αFilament bundlesSignal-regulated kinase 1/2Four-nucleotide insertionDisease mechanismsContractile smooth muscle cellsStem cell linesPluripotent stem cellsPluripotent stem cell linePlatelet-derived growth factorRhoA signalingVascular smooth muscle cellsRecombinant proteinsKinase 1/2Elastin geneELN geneWilliams-Beuren syndromeBrdU analysisSupravalvular aortic stenosisStem cellsHigh proliferation rate
2011
Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells
Ren Y, Lee MY, Schliffke S, Paavola J, Amos PJ, Ge X, Ye M, Zhu S, Senyei G, Lum L, Ehrlich BE, Qyang Y. Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells. Journal Of Molecular And Cellular Cardiology 2011, 51: 280-287. PMID: 21569778, PMCID: PMC3334336, DOI: 10.1016/j.yjmcc.2011.04.012.Peer-Reviewed Original ResearchConceptsHuman iPS cellsAction potential durationHuman iPS cell-derived cardiomyocytesSmall-molecule Wnt inhibitorsIPS cellsPluripotent stem cellsIPS cell-derived cardiomyocytesBMP-4Cardiac disease mechanismsCardiac differentiationCell-derived cardiomyocytesPotential durationBone morphogenetic protein 4Stem cellsWnt inhibitorsCardiomyocyte action potential durationHuman pluripotent stem cellsInduced pluripotent stem cellsHuman induced pluripotent stem cellsDisease mechanismsTranslational cardiac researchIntracellular calcium imagingEfficient cardiac differentiationWnt/β-cateninProduction of cardiomyocytes