p53 protein aggregation promotes platinum resistance in ovarian cancer
Yang-Hartwich Y, Soteras MG, Lin ZP, Holmberg J, Sumi N, Craveiro V, Liang M, Romanoff E, Bingham J, Garofalo F, Alvero A, Mor G. p53 protein aggregation promotes platinum resistance in ovarian cancer. Oncogene 2014, 34: 3605-3616. PMID: 25263447, DOI: 10.1038/onc.2014.296.Peer-Reviewed Original ResearchConceptsPro-apoptotic functionP53 aggregationProtein aggregationP53 aggregatesNormal transcriptional activationTwo-dimensional gel electrophoresisCancer cellsCancer cell survivalKey transcriptional factorGenetic mutationsHigh-grade serous ovarian carcinomaP53 inactivationP53 proteinStem cell propertiesCancer stem cell propertiesCellular homeostasisTranscriptional activationCancer stem cellsTranscriptional factorsTumor-initiating capacityP53 turnoverCell survivalHGSOC cellsStem cellsPotential therapeutic targetDetection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation
Yang-Hartwich Y, Romanoff E, Bingham J, Alvero AB, Mor G. Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation. Methods In Molecular Biology 2014, 1219: 87-93. PMID: 25308264, DOI: 10.1007/978-1-4939-1661-0_8.Peer-Reviewed Original ResearchConceptsTranscriptional activityP53 transcriptional activityChromatin immunoprecipitationTranscription-dependent activityDNA-binding domainExpression of hundredsImportant cellular functionsKey transcriptional mediatorAbility of p53Protein's transcriptional activityP53-induced apoptosisResult of mutationsGenome integrityCellular homeostasisCellular functionsTranscriptional mediatorsTarget genesPromoter regionApoptotic genesCell deathReal-time quantitative polymerase chain reactionCancer cellsQuantitative polymerase chain reactionImmunoprecipitationGenes