2002
Inhibition of cholesterol biosynthesis by Δ22-unsaturated phytosterols via competitive inhibition of sterol Δ24-reductase in mammalian cells
FERNÁNDEZ C, SUÁREZ Y, FERRUELO AJ, GÓMEZ-CORONADO D, LASUNCIÓN MA. Inhibition of cholesterol biosynthesis by Δ22-unsaturated phytosterols via competitive inhibition of sterol Δ24-reductase in mammalian cells. Biochemical Journal 2002, 366: 109-119. PMID: 12162789, PMCID: PMC1222779, DOI: 10.1042/bj20011777.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveCaco-2 CellsCholesterolChromatography, High Pressure LiquidDose-Response Relationship, DrugHL-60 CellsHumansHypolipidemic AgentsKineticsMacrophagesMaleMiceMicrosomes, LiverModels, ChemicalOxidoreductasesPhytosterolsRatsRats, Sprague-DawleyStigmasterolTime FactorsTumor Cells, CulturedConceptsCholesterol biosynthesisCholesterol-lowering actionCholesterol-lowering agentsEffective hypocholesterolaemic agentHL-60 human cell linesRat liver microsomesIntestinal absorptionDietary phytosterolsLiver microsomesHypocholesterolaemic agentsLow intracellular concentrationsCaco-2Inhibition of sterolIncorporation of radioactivityHuman cell linesCell linesInhibitionSaturated side chainIntracellular concentrationCholesterolRelevant concentrationsRadioactivity incorporationPresent studyStrong inhibitionCompetitive inhibition
1999
Cholesterol starvation decreases P34cdc2 kinase activity and arrests the cell cycle at G2
Martínez‐Botas J, Suárez Y, Ferruelo A, Gómez‐Coronado D, Lasunció M. Cholesterol starvation decreases P34cdc2 kinase activity and arrests the cell cycle at G2. The FASEB Journal 1999, 13: 1359-1370. PMID: 10428760, DOI: 10.1096/fasebj.13.11.1359.Peer-Reviewed Original Research
1998
Induction of apoptosis in p53-null HL-60 cells by inhibition of lanosterol 14-α demethylase
Martínez-Botas J, Ferruelo A, Suárez Y, Gómez-Coronado D, Lasunción M. Induction of apoptosis in p53-null HL-60 cells by inhibition of lanosterol 14-α demethylase. Biochimie 1998, 80: 887-894. PMID: 9893947, DOI: 10.1016/s0300-9084(00)88884-7.Peer-Reviewed Original Research