Characterization and Application of a New Optical Probe for Membrane Lipid Domains
Jin L, Millard AC, Wuskell JP, Dong X, Wu D, Clark HA, Loew LM. Characterization and Application of a New Optical Probe for Membrane Lipid Domains. Biophysical Journal 2006, 90: 2563-2575. PMID: 16415047, PMCID: PMC1403187, DOI: 10.1529/biophysj.105.072884.Peer-Reviewed Original ResearchConceptsEmission spectraStrong second harmonic generation (SHG) signalNew optical probeSecond harmonic generation signalNonlinear optical microscopyHarmonic generation signalLiquid-disordered phasesLarge unilamellar lipid vesiclesOptical propertiesOptical probeGeneration signalSensitive probeBlue shiftOptical microscopyGiant unilamellar vesiclesLiquid-ordered stateDye moleculesUnilamellar lipid vesiclesLiquid-disordered domainsLipid domainsMolecular packingSpectraLipid membranesRed fluorescencePhase separation