Featured Publications
Size-dependent activation of CAR-T cells
Xiao Q, Zhang X, Tu L, Cao J, Hinrichs CS, Su X. Size-dependent activation of CAR-T cells. Science Immunology 2022, 7: eabl3995. PMID: 35930653, PMCID: PMC9678385, DOI: 10.1126/sciimmunol.abl3995.Peer-Reviewed Original ResearchConceptsIntermembrane spacePlasma membraneIntracellular domainExtracellular domainMolecular mechanismsLarge isoformDownstream pathwaysT cell activationVariety of cancersCaR's extracellular domainAntigen engagementAntigen receptorSize differencesCell activationActivation of CARActivationMouse lymphoma modelCD45Antigen signalCellsLymphoma modelKinasePhosphorylationCD45RABCViral infectionPLCγ1 promotes phase separation of T cell signaling components
Zeng L, Palaia I, Šarić A, Su X. PLCγ1 promotes phase separation of T cell signaling components. Journal Of Cell Biology 2021, 220: e202009154. PMID: 33929486, PMCID: PMC8094118, DOI: 10.1083/jcb.202009154.Peer-Reviewed Original ResearchConceptsTCR signal transductionT-cell receptor pathwayLiquid-like condensatesKey adaptor proteinLAT clusteringLAT complexSH2 domainAdaptor proteinPhosphatase CD45Signal transductionTCR pathwayPhospholipase Cγ1ERK activationProtein compositionBiophysical principlesPLCγ1Critical functionsReceptor pathwayPathwayLATMajor componentT cellsCellsActivationDephosphorylation
2020
Imaging Chimeric Antigen Receptor (CAR) Activation
Libby KA, Su X. Imaging Chimeric Antigen Receptor (CAR) Activation. Methods In Molecular Biology 2020, 2111: 153-160. PMID: 31933206, DOI: 10.1007/978-1-0716-0266-9_13.Peer-Reviewed Original Research
2019
A composition-dependent molecular clutch between T cell signaling condensates and actin
Ditlev JA, Vega AR, Köster DV, Su X, Tani T, Lakoduk AM, Vale RD, Mayor S, Jaqaman K, Rosen MK. A composition-dependent molecular clutch between T cell signaling condensates and actin. ELife 2019, 8: e42695. PMID: 31268421, PMCID: PMC6624021, DOI: 10.7554/elife.42695.Peer-Reviewed Original Research
2016
Phase separation of signaling molecules promotes T cell receptor signal transduction
Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton J, Rosen MK, Vale RD. Phase separation of signaling molecules promotes T cell receptor signal transduction. Science 2016, 352: 595-599. PMID: 27056844, PMCID: PMC4892427, DOI: 10.1126/science.aad9964.Peer-Reviewed Original ResearchConceptsT cell receptor (TCR) signal transductionHuman Jurkat T cellsActin filament assemblySubmicrometer-sized clustersReceptor signal transductionT cell receptor activationProtein phase separationJurkat T cellsCell receptor activationCell surface receptorsTCR phosphorylationActin regulatorsActin assemblySignal transductionFilament assemblyBiochemical compartmentsFunctional consequencesSurface receptorsModel membranesReceptor activationAssemblyActivationTransductionKinasePhosphorylation