2010
Experience‐dependent plasticity in hypocretin/orexin neurones: re‐setting arousal threshold
Gao X, Wang AH. Experience‐dependent plasticity in hypocretin/orexin neurones: re‐setting arousal threshold. Acta Physiologica 2010, 198: 251-262. PMID: 19785627, PMCID: PMC2860674, DOI: 10.1111/j.1748-1716.2009.02047.x.Peer-Reviewed Original ResearchConceptsExperience-dependent plasticityExperience-dependent changesEnvironmental cuesArousal levelMaintenance of arousalBrain areasAddictive drugsBrain functionSynaptic architectureLateral hypothalamusAnimal behaviorExtensive innervationNeuropeptide hypocretinArousal thresholdCore processesSynaptic plasticityHypocretin/orexin neuronesArousalAnimal survivalEnvironmental factorsPlasticityBehavioral factorsNeuronesBehavioral changesOrexin neurones
2002
Hypocretin (orexin) enhances neuron activity and cell synchrony in developing mouse GFP‐expressing locus coeruleus
van den Pol A, Ghosh PK, Liu R, Li Y, Aghajanian GK, Gao X. Hypocretin (orexin) enhances neuron activity and cell synchrony in developing mouse GFP‐expressing locus coeruleus. The Journal Of Physiology 2002, 541: 169-185. PMID: 12015428, PMCID: PMC2290314, DOI: 10.1113/jphysiol.2002.017426.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCells, CulturedDose-Response Relationship, DrugElectrophysiologyFemaleGreen Fluorescent ProteinsImmunohistochemistryIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsLocus CoeruleusLuminescent ProteinsMembrane PotentialsMiceMice, TransgenicMicroscopy, ConfocalNeuronsNeuropeptidesNeurotransmitter AgentsOrexinsPatch-Clamp TechniquesPregnancyTyrosine 3-MonooxygenaseConceptsLocus coeruleusHypocretin-1Mouse prion promoterGlutamate receptor antagonistsPresence of TTXImmunoreactive tyrosine hydroxylaseRat locus coeruleusNeonatal mouse brainPostnatal day 0Higher brain centersNew transgenic mouseSingle-unit recordingsInward cation currentMembrane potentialDual cell recordingsImproved cognitive performanceNoradrenergic neuronsLC neuronsHypocretin neuronsImmature neuronsPrion promoterReceptor antagonistMouse slicesTyrosine hydroxylaseExcitatory mechanisms
2001
GABA, Not Glutamate, a Primary Transmitter Driving Action Potentials in Developing Hypothalamic Neurons
Gao X, Van Den Pol A. GABA, Not Glutamate, a Primary Transmitter Driving Action Potentials in Developing Hypothalamic Neurons. Journal Of Neurophysiology 2001, 85: 425-434. PMID: 11152743, DOI: 10.1152/jn.2001.85.1.425.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBicucullineCells, CulturedChloridesExcitatory Amino Acid AntagonistsGABA-A Receptor AntagonistsGamma-Aminobutyric AcidGlutamic AcidGramicidinHypothalamusImmunohistochemistryIn Vitro TechniquesMiceNeuronsNeurotransmitter AgentsPatch-Clamp TechniquesReceptors, GlutamateSpinal CordConceptsHypothalamic neuronsReceptor antagonist bicucullinePrimary inhibitory neurotransmitterMouse hypothalamic neuronsPrimary excitatory transmitterAntagonist bicucullineExcitatory transmitterGABA circuitsGlutamate transmissionInhibitory neurotransmitterMature brainBrain slicesSynaptic releaseNeuronal activitySpike activityNeuronal connectionsSpike frequencyExtracellular recordingsAction potentialsAntagonist 2Brain developmentHebbian mechanismsIntracellular ClNeuronsGABA
1998
Presynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin
van den Pol A, Gao X, Obrietan K, Kilduff T, Belousov A. Presynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin. Journal Of Neuroscience 1998, 18: 7962-7971. PMID: 9742163, PMCID: PMC6793026, DOI: 10.1523/jneurosci.18-19-07962.1998.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArcuate Nucleus of HypothalamusCadmiumCalciumCarrier ProteinsCells, CulturedDose-Response Relationship, DrugElectric StimulationEnzyme InhibitorsEvoked PotentialsFeeding BehaviorFluorescent DyesFura-2Gamma-Aminobutyric AcidGlutamic AcidIntracellular Signaling Peptides and ProteinsMembrane PotentialsMolecular Sequence DataNeuronsNeuropeptidesNeurosecretory SystemsNeurotransmitter AgentsOrexinsPatch-Clamp TechniquesPresynaptic TerminalsSignal TransductionTetrodotoxinThapsigarginConceptsLateral hypothalamusSynaptic activityNeuroendocrine neuronsWhole-cell patch-clamp recordingsNew hypothalamic peptidesLateral hypothalamic neuronsAmino acid transmittersHypothalamic regulatory systemPatch-clamp recordingsHypocretin/orexinPerifornical areaPostsynaptic actionsOrexigenic peptideHypocretin-1Hypothalamic circuitsHypocretin peptidesHypothalamic neuronsHypothalamic slicesArcuate nucleusHypocretin-2Postsynaptic receptorsAxonal inputsHypothalamic peptidesFeeding centerAxon terminalsThe hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity
de Lecea L, Kilduff TS, Peyron C, Gao X, Foye PE, Danielson PE, Fukuhara C, Battenberg E, Gautvik VT, Bartlett FS, Frankel WN, van den Pol AN, Bloom FE, Gautvik KM, Sutcliffe JG. The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 322-327. PMID: 9419374, PMCID: PMC18213, DOI: 10.1073/pnas.95.1.322.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCarrier ProteinsChromosomesConsensus SequenceHomeostasisHypothalamus, PosteriorIntracellular Signaling Peptides and ProteinsMaleMiceMolecular Sequence DataNeuronsNeuropeptidesNeurotransmitter AgentsOrexinsProtein PrecursorsRatsRats, WistarSecretinSynaptic VesiclesConceptsGut hormone secretinLateral hypothalamic areaNeuronal cell bodiesLarge granular vesiclesPosterior hypothalamusHypothalamic areaHypothalamic neuronsHippocampal neuronsCell bodiesGranular vesiclesHcrt peptidesHormone secretinNeuronsHypocretinNeuroexcitatory activityPeptidesPutative precursorBrainstemThalamusHypothalamusImmunoreactivityCNSNeurotransmittersSecretinProtein products