2002
Hypocretin/Orexin Excites Hypocretin Neurons via a Local Glutamate Neuron—A Potential Mechanism for Orchestrating the Hypothalamic Arousal System
Li Y, Gao X, Sakurai T, van den Pol AN. Hypocretin/Orexin Excites Hypocretin Neurons via a Local Glutamate Neuron—A Potential Mechanism for Orchestrating the Hypothalamic Arousal System. Neuron 2002, 36: 1169-1181. PMID: 12495630, DOI: 10.1016/s0896-6273(02)01132-7.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsArousalCarrier ProteinsExcitatory Amino Acid AgonistsFeedbackGABA AgonistsGamma-Aminobutyric AcidGenes, ReporterGlutamic AcidGreen Fluorescent ProteinsHypothalamusImmunohistochemistryIntracellular Signaling Peptides and ProteinsLuminescent ProteinsMiceMice, TransgenicNeural InhibitionNeural PathwaysNeuronsNeuropeptidesOrexinsPresynaptic TerminalsSodium Channel BlockersSynaptic TransmissionConceptsHypothalamic arousal systemsHypocretin neuronsArousal systemSpike frequencyLocal glutamatergic neuronsMouse brain slicesGlutamate releaseGlutamatergic neuronsHypocretin-1Hypocretin cellsPresynaptic facilitationBrain slicesNeuron activityOutward currentsEnergy homeostasisModulates sleepNeuronsPotential mechanismsDirect effectPossible mechanismCellsNarcolepsyHypocretinNorepinephrineAcetylcholineHypocretin (orexin) enhances neuron activity and cell synchrony in developing mouse GFP‐expressing locus coeruleus
van den Pol A, Ghosh PK, Liu R, Li Y, Aghajanian GK, Gao X. Hypocretin (orexin) enhances neuron activity and cell synchrony in developing mouse GFP‐expressing locus coeruleus. The Journal Of Physiology 2002, 541: 169-185. PMID: 12015428, PMCID: PMC2290314, DOI: 10.1113/jphysiol.2002.017426.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCells, CulturedDose-Response Relationship, DrugElectrophysiologyFemaleGreen Fluorescent ProteinsImmunohistochemistryIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsLocus CoeruleusLuminescent ProteinsMembrane PotentialsMiceMice, TransgenicMicroscopy, ConfocalNeuronsNeuropeptidesNeurotransmitter AgentsOrexinsPatch-Clamp TechniquesPregnancyTyrosine 3-MonooxygenaseConceptsLocus coeruleusHypocretin-1Mouse prion promoterGlutamate receptor antagonistsPresence of TTXImmunoreactive tyrosine hydroxylaseRat locus coeruleusNeonatal mouse brainPostnatal day 0Higher brain centersNew transgenic mouseSingle-unit recordingsInward cation currentMembrane potentialDual cell recordingsImproved cognitive performanceNoradrenergic neuronsLC neuronsHypocretin neuronsImmature neuronsPrion promoterReceptor antagonistMouse slicesTyrosine hydroxylaseExcitatory mechanisms
2001
Lateral hypothalamus: Early developmental expression and response to hypocretin (orexin)
Van Den Pol A, Patrylo P, Ghosh P, Gao X. Lateral hypothalamus: Early developmental expression and response to hypocretin (orexin). The Journal Of Comparative Neurology 2001, 433: 349-363. PMID: 11298360, DOI: 10.1002/cne.1144.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAnimals, NewbornBrainCalciumCarrier ProteinsCells, CulturedElectrophysiologyEmbryo, MammalianHypothalamic Area, LateralImmunohistochemistryIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsNeuronsNeuropeptidesOrexin ReceptorsOrexinsRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledReceptors, NeuropeptideRNA, MessengerConceptsLateral hypothalamic areaSynaptic activityLH neuronsNeuronal activitySleep regulationWhole-cell patch-clamp recordingsRobust increaseAdult central nervous systemEndocrine controlPostnatal day 1Day of birthCentral nervous systemPatch-clamp recordingsVoltage-clamp recordingsEmbryonic day 19Hypocretin-1Excitatory influenceHypothalamic areaHypocretin-2Spinal cordMature brainFood intakeHypocretin systemLH cellsReceptor mRNA
1998
Presynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin
van den Pol A, Gao X, Obrietan K, Kilduff T, Belousov A. Presynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin. Journal Of Neuroscience 1998, 18: 7962-7971. PMID: 9742163, PMCID: PMC6793026, DOI: 10.1523/jneurosci.18-19-07962.1998.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArcuate Nucleus of HypothalamusCadmiumCalciumCarrier ProteinsCells, CulturedDose-Response Relationship, DrugElectric StimulationEnzyme InhibitorsEvoked PotentialsFeeding BehaviorFluorescent DyesFura-2Gamma-Aminobutyric AcidGlutamic AcidIntracellular Signaling Peptides and ProteinsMembrane PotentialsMolecular Sequence DataNeuronsNeuropeptidesNeurosecretory SystemsNeurotransmitter AgentsOrexinsPatch-Clamp TechniquesPresynaptic TerminalsSignal TransductionTetrodotoxinThapsigarginConceptsLateral hypothalamusSynaptic activityNeuroendocrine neuronsWhole-cell patch-clamp recordingsNew hypothalamic peptidesLateral hypothalamic neuronsAmino acid transmittersHypothalamic regulatory systemPatch-clamp recordingsHypocretin/orexinPerifornical areaPostsynaptic actionsOrexigenic peptideHypocretin-1Hypothalamic circuitsHypocretin peptidesHypothalamic neuronsHypothalamic slicesArcuate nucleusHypocretin-2Postsynaptic receptorsAxonal inputsHypothalamic peptidesFeeding centerAxon terminals