2021
Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps
Chen B, Scurrah CR, McKinley ET, Simmons AJ, Ramirez-Solano MA, Zhu X, Markham NO, Heiser CN, Vega PN, Rolong A, Kim H, Sheng Q, Drewes JL, Zhou Y, Southard-Smith AN, Xu Y, Ro J, Jones AL, Revetta F, Berry LD, Niitsu H, Islam M, Pelka K, Hofree M, Chen JH, Sarkizova S, Ng K, Giannakis M, Boland GM, Aguirre AJ, Anderson AC, Rozenblatt-Rosen O, Regev A, Hacohen N, Kawasaki K, Sato T, Goettel JA, Grady WM, Zheng W, Washington MK, Cai Q, Sears CL, Goldenring JR, Franklin JL, Su T, Huh WJ, Vandekar S, Roland JT, Liu Q, Coffey RJ, Shrubsole MJ, Lau KS. Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell 2021, 184: 6262-6280.e26. PMID: 34910928, PMCID: PMC8941949, DOI: 10.1016/j.cell.2021.11.031.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAdenomaAdultAgedAnimalsCarcinogenesisCell DeathCell DifferentiationColonic PolypsColorectal NeoplasmsDisease ProgressionFemaleGene Expression Regulation, NeoplasticGene Regulatory NetworksGenetic HeterogeneityHumansMaleMiceMiddle AgedMutationNeoplastic Stem CellsReproducibility of ResultsRNA-SeqSingle-Cell AnalysisTumor MicroenvironmentConceptsHuman colorectal polypsColorectal cancerColorectal polypsPrevention of CRCMicrosatellite-unstable colorectal cancersUnstable colorectal cancersGastric metaplasiaImmune microenvironmentTumor cell differentiation statusImmune cellsPrecision surveillancePrecursor polypsSerrated polypsConventional adenomasStem cell propertiesMalignant progressionImmunogenic potentialPolypsTumor cellsTherapeutic insightsCell differentiation statusCellular originMetaplasiaAdenomasMolecular heterogeneity
2020
Noggin regulates foregut progenitor cell programming and mis-expression leads to esophageal atresia
Pinzon-Guzman C, Sangadala S, Riera KM, Popova EY, Manning E, Huh WJ, Alexander MS, Shelton JS, Boden SD, Goldenring JR. Noggin regulates foregut progenitor cell programming and mis-expression leads to esophageal atresia. Journal Of Clinical Investigation 2020, 130: 4396-4410. PMID: 32427591, PMCID: PMC7410075, DOI: 10.1172/jci123597.Peer-Reviewed Original Research
2016
Hepatocyte nuclear factor 4α is required for cell differentiation and homeostasis in the adult mouse gastric epithelium
Moore BD, Khurana SS, Huh WJ, Mills JC. Hepatocyte nuclear factor 4α is required for cell differentiation and homeostasis in the adult mouse gastric epithelium. AJP Gastrointestinal And Liver Physiology 2016, 311: g267-g275. PMID: 27340127, PMCID: PMC5007292, DOI: 10.1152/ajpgi.00195.2016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsBinding SitesCell DifferentiationCell LineCell ProliferationEpithelial CellsGastric MucosaGene Expression RegulationGenotypeHepatocyte Nuclear Factor 4HomeostasisHumansMice, KnockoutPhenotypePromoter Regions, GeneticSignal TransductionTransfectionX-Box Binding Protein 1ConceptsZymogenic chief cellsAdult mouse stomachX-box binding protein 1Gastric unitsRole of HNF4αStem cell zoneHepatocyte nuclear factor 4 alphaLoss of HNF4αBinding protein 1Nuclear factor 4 alphaHepatocyte nuclear factor 4αHuman gastric cancer cellsMouse stomachTranscriptional regulatorsChromatin immunoprecipitationNuclear factor 4αGastric cancer cellsXBP1 mRNAHNF4α proteinTranscription factorsUpstream regulationMouse gastric epitheliumHuman gastric cellsCell differentiationAdditional phenotypes
2010
XBP1 Controls Maturation of Gastric Zymogenic Cells by Induction of MIST1 and Expansion of the Rough Endoplasmic Reticulum
Huh WJ, Esen E, Geahlen JH, Bredemeyer AJ, Lee A, Shi G, Konieczny SF, Glimcher LH, Mills JC. XBP1 Controls Maturation of Gastric Zymogenic Cells by Induction of MIST1 and Expansion of the Rough Endoplasmic Reticulum. Gastroenterology 2010, 139: 2038-2049. PMID: 20816838, PMCID: PMC2997137, DOI: 10.1053/j.gastro.2010.08.050.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsCell DifferentiationCell LineChief Cells, GastricDNA-Binding ProteinsEndoplasmic Reticulum, RoughIntegrasesMiceMice, KnockoutMicroscopy, Electron, TransmissionPromoter Regions, GeneticRegulatory Factor X Transcription FactorsSecretory VesiclesStem CellsTranscription FactorsX-Box Binding Protein 1ConceptsEndoplasmic reticulumZymogenic cellsRough endoplasmic reticulumNC markersGastric zymogenic cellsTranscription factor XBP1Large secretory vesiclesLamellar rough endoplasmic reticulumAbsence of XBP1Transcription factor MIST1Cell shape abnormalitiesCre-loxP systemTranscriptional regulationChromatin immunoprecipitationTranscriptional activationSecretory vesiclesGastric cell linesMist1Neck cellsXBP1Cell typesImmunoblot analysisCell linesQuantitative reverse transcriptase-polymerase chain reactionMIST1 expression
2008
The gastric epithelial progenitor cell niche and differentiation of the zymogenic (chief) cell lineage
Bredemeyer AJ, Geahlen JH, Weis VG, Huh WJ, Zinselmeyer BH, Srivatsan S, Miller MJ, Shaw AS, Mills JC. The gastric epithelial progenitor cell niche and differentiation of the zymogenic (chief) cell lineage. Developmental Biology 2008, 325: 211-224. PMID: 19013146, PMCID: PMC2634829, DOI: 10.1016/j.ydbio.2008.10.025.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBasic Helix-Loop-Helix Transcription FactorsCadherinsCell DifferentiationCell LineageCell PolarityCell ProliferationChief Cells, GastricCytoskeletal ProteinsEpithelial CellsIntercellular JunctionsMiceModels, BiologicalParietal Cells, GastricProtein BindingProtein TransportStem Cell NicheStem CellsConceptsZymogenic cellsProgenitor nicheCell fate decisionsE-cadherin localizationZymogenic cell lineagesMammalian gastrointestinal tractProgenitor cell nicheParietal cellsLineage progenitor cellsKey cellular componentsFate decisionsDiverse lineagesProgenitor stateDifferentiated cell markersCell nicheGastric parietal cellsCell lineagesPremature expressionCellular componentsGastric unitsLineagesNicheGenetic ablationLineage cellsProgenitor cells
2006
Location, allocation, relocation: isolating adult tissue stem cells in three dimensions
Huh WJ, Pan XO, Mysorekar IU, Mills JC. Location, allocation, relocation: isolating adult tissue stem cells in three dimensions. Current Opinion In Biotechnology 2006, 17: 511-517. PMID: 16889955, DOI: 10.1016/j.copbio.2006.07.002.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements