Identification of exopolysaccharide‐deficient mutants of Mycoplasma pulmonis
Daubenspeck J, Bolland J, Luo W, Simmons W, Dybvig K. Identification of exopolysaccharide‐deficient mutants of Mycoplasma pulmonis. Molecular Microbiology 2009, 72: 1235-1245. PMID: 19432800, PMCID: PMC2752295, DOI: 10.1111/j.1365-2958.2009.06720.x.Peer-Reviewed Original ResearchConceptsOpen reading frameExopolysaccharide-deficient mutantsSynthesis of exopolysaccharidesTerminal beta-linked galactose residuesA549 lung cell lineHeterodimeric pairTransposon librarySecond exopolysaccharideReading frameMutantsPhenotypic analysisLung cell linesDecreased associationN-acetylglucosamineCell linesExopolysaccharideCapsular exopolysaccharideLectin affinity chromatographyGalactose residuesComplex carbohydratesNew pathwayPermeasesMouse lungMollicutesTransposon