2022
Activity of a direct VTA to ventral pallidum GABA pathway encodes unconditioned reward value and sustains motivation for reward
Zhou WL, Kim K, Ali F, Pittenger ST, Calarco CA, Mineur YS, Ramakrishnan C, Deisseroth K, Kwan AC, Picciotto MR. Activity of a direct VTA to ventral pallidum GABA pathway encodes unconditioned reward value and sustains motivation for reward. Science Advances 2022, 8: eabm5217. PMID: 36260661, PMCID: PMC9581470, DOI: 10.1126/sciadv.abm5217.Peer-Reviewed Original ResearchHippocampal acetylcholine modulates stress-related behaviors independent of specific cholinergic inputs
Mineur YS, Mose TN, Vanopdenbosch L, Etherington IM, Ogbejesi C, Islam A, Pineda CM, Crouse RB, Zhou W, Thompson DC, Bentham MP, Picciotto MR. Hippocampal acetylcholine modulates stress-related behaviors independent of specific cholinergic inputs. Molecular Psychiatry 2022, 27: 1829-1838. PMID: 34997190, PMCID: PMC9106825, DOI: 10.1038/s41380-021-01404-7.Peer-Reviewed Original ResearchConceptsStress-related behaviorsCholinergic inputMedial septum/diagonal bandBehavioral effectsBrain ACh levelsChAT-positive neuronsSelective chemogenetic activationMuscarinic ACh receptorsDepression-like symptomsSignificant behavioral effectsHippocampal acetylcholineMaladaptive behavioral responsesAntidepressant effectsCholinergic neuronsACh releaseChemogenetic activationChemogenetic inhibitionCholinergic antagonistsLocal infusionAcetylcholine levelsACh levelsDiagonal bandACh receptorsHippocampal neuronsPharmacological approaches
2020
Local glutamate-mediated dendritic plateau potentials change the state of the cortical pyramidal neuron
Gao PP, Graham JW, Zhou WL, Jang J, Angulo S, Dura-Bernal S, Hines M, Lytton WW, Antic SD. Local glutamate-mediated dendritic plateau potentials change the state of the cortical pyramidal neuron. Journal Of Neurophysiology 2020, 125: 23-42. PMID: 33085562, PMCID: PMC8087381, DOI: 10.1152/jn.00734.2019.Peer-Reviewed Original ResearchConceptsCortical pyramidal neuronsDendritic plateau potentialsPyramidal neuronsPlateau potentialsCell bodiesBasal dendritesAfferent inputDendritic potentialsAction potentialsThin basal dendritesSomatic input resistanceThin dendritic branchesLocal dendritic potentialNeuronal statesGlutamatergic inputsFunctional neuronal ensemblesTau changesCortical neuronsVoltage-sensitive dyeDendritic spikesMembrane time constantStimulation intensityOblique branchNeuronsInput resistance
2018
Molecular and cellular characterization of nicotinic acetylcholine receptor subtypes in the arcuate nucleus of the mouse hypothalamus
Calarco CA, Li Z, Taylor SR, Lee S, Zhou W, Friedman JM, Mineur YS, Gotti C, Picciotto MR. Molecular and cellular characterization of nicotinic acetylcholine receptor subtypes in the arcuate nucleus of the mouse hypothalamus. European Journal Of Neuroscience 2018, 48: 1600-1619. PMID: 29791746, PMCID: PMC6251769, DOI: 10.1111/ejn.13966.Peer-Reviewed Original ResearchPOMC cellsArcuate nucleusFood intakeOrexigenic agouti-related peptideNicotinic acetylcholine receptor subtypesAnorexigenic pro-opiomelanocortin (POMC) neuronsDecrease food intakePro-opiomelanocortin (POMC) neuronsAcetylcholine receptor subtypesMRNA levelsAgouti-related peptideNicotinic agonist cytisineNicotinic acetylcholine receptorsSubunit mRNA levelsNeuronal cell typesAnorexigenic neuronsNicotinic regulationNicotinic modulationSmall hairpin RNAHypothalamic regionsReal-time PCRReceptor subtypesAgonist cytisineΑ7 subunitAcetylcholine receptorsStriatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons
Li D, Musante V, Zhou W, Picciotto MR, Nairn AC. Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons. Journal Of Biological Chemistry 2018, 293: 11179-11194. PMID: 29802198, PMCID: PMC6052221, DOI: 10.1074/jbc.ra117.001519.Peer-Reviewed Original ResearchConceptsSerine/threonine phosphatase PP2AStriatin-interacting phosphataseRNA knockdown approachB subunitSTRIPAK complexPhosphatase PP2AProtein phosphataseMultiprotein complexesKnockdown approachStriatin familyMutant constructsStriatal neuronal culturesPP2ANeuronal developmentPrimary striatal neuronal culturesDendritic phenotypeKnockdown modelSynapse formationSubunitsSpine developmentSelective roleReduced expressionNeuron maturationNeuronal culturesStriatal neurons
2017
The stochastic nature of action potential backpropagation in apical tuft dendrites
Short SM, Oikonomou KD, Zhou WL, Acker CD, Popovic MA, Zecevic D, Antic SD. The stochastic nature of action potential backpropagation in apical tuft dendrites. Journal Of Neurophysiology 2017, 118: 1394-1414. PMID: 28566465, PMCID: PMC5558024, DOI: 10.1152/jn.00800.2016.Peer-Reviewed Original ResearchConceptsDendritic CaPyramidal neuronsApical tuftCortical pyramidal neuronsAction potential backpropagationRat brain slicesSpontaneous synaptic inputsDifferent cortical layersVoltage-gated CaAP backpropagationAP burstsGlutamate iontophoresisSynaptic contactsSomatic APsApical trunkAP frequencyBrain slicesDendritic physiologySynaptic inputsCortical layersChannel inactivationLocal NaNeuronsSpike-timing dependent plasticityTrials
2015
Antidepressant-like effects of guanfacine and sex-specific differences in effects on c-fos immunoreactivity and paired-pulse ratio in male and female mice
Mineur YS, Bentham MP, Zhou WL, Plantenga ME, McKee SA, Picciotto MR. Antidepressant-like effects of guanfacine and sex-specific differences in effects on c-fos immunoreactivity and paired-pulse ratio in male and female mice. Psychopharmacology 2015, 232: 3539-3549. PMID: 26146014, PMCID: PMC4561580, DOI: 10.1007/s00213-015-4001-3.Peer-Reviewed Original ResearchConceptsAntidepressant-like effectsPaired-pulse ratioC-Fos immunoreactivityPrefrontal cortexSwim testBrain areasRobust antidepressant-like effectsBrain regionsSex differencesMale C57BL/6J miceDepression-like behaviorEffects of guanfacineAcetylcholinesterase inhibitor physostigmineLight/dark boxBaseline sex differencesC-fos expressionDepression-like stateCritical brain regionsDifferent brain areasSex-specific changesAntidepressant efficacyCholinergic controlInhibitor physostigmineC57BL/6J miceAgonist guanfacineAcetylcholine Acts through Nicotinic Receptors to Enhance the Firing Rate of a Subset of Hypocretin Neurons in the Mouse Hypothalamus through Distinct Presynaptic and Postsynaptic Mechanisms1,2
Zhou WL, Gao XB, Picciotto MR. Acetylcholine Acts through Nicotinic Receptors to Enhance the Firing Rate of a Subset of Hypocretin Neurons in the Mouse Hypothalamus through Distinct Presynaptic and Postsynaptic Mechanisms1,2. ENeuro 2015, 2: eneuro.0052-14.2015. PMID: 26322330, PMCID: PMC4551500, DOI: 10.1523/eneuro.0052-14.2015.Peer-Reviewed Original ResearchHypocretin neuronsMiniature EPSCsPostsynaptic nAChRsFiring rateHypocretin/orexin neuronsFrequency of mEPSCsAntagonism of nAChRsPhasic ACh releasePresence of atropineApplication of AChInternal calcium releaseNicotinic acetylcholine receptorsMouse brain slicesOne-thirdAddiction-related behaviorsPresynaptic AChPresynaptic nAChRsOrexin neuronsACh releaseGlutamatergic inputsPostsynaptic modulationPostsynaptic mechanismsMEPSC frequencyTonic activationBrain slices
2014
Branch specific and spike-order specific action potential invasion in basal, oblique, and apical dendrites of cortical pyramidal neurons
Zhou W, Short S, Rich M, Oikonomou K, Singh M, Sterjanaj E, Antic S. Branch specific and spike-order specific action potential invasion in basal, oblique, and apical dendrites of cortical pyramidal neurons. Neurophotonics 2014, 2: 021006-021006. PMID: 26157997, PMCID: PMC4478750, DOI: 10.1117/1.nph.2.2.021006.Peer-Reviewed Original ResearchPyramidal neuronsAction potentialsDendritic branchesTrains of APsCortical pyramidal neuronsL5 pyramidal neuronsSame cortical layerNeocortical pyramidal neuronsAction potential invasionThin dendritic branchesAP backpropagationApical dendritesAP trainsApical trunkSame neuronsDendritic diameterDistal synapsesCalcium transientsVariable efficacyCalcium imagingCortical layersDendritic treeDendritic voltageOblique branchMultisite optical recordingsConnexin hemichannels contribute to spontaneous electrical activity in the human fetal cortex
Moore A, Zhou W, Sirois C, Belinsky G, Zecevic N, Antic S. Connexin hemichannels contribute to spontaneous electrical activity in the human fetal cortex. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: e3919-e3928. PMID: 25197082, PMCID: PMC4169969, DOI: 10.1073/pnas.1405253111.Peer-Reviewed Original ResearchConceptsHuman fetal cortexAction potential firingFetal cortexSP neuronsSpontaneous activitySpontaneous depolarizationsYoung postmitotic neuronsGlycinergic synaptic transmissionConnexin-based gap junctionsElectrical activityAcute cortical slicesSpontaneous electrical activityWhole-cell recordingsConnexin hemichannelsGap junctionsHuman subplate neuronsPresence of connexinsSubplate neuronsSecond trimesterCortical slicesSynaptic contactsSynaptic transmissionImmunohistochemical analysisSP zonePostmitotic neurons
2012
Palette of fluorinated voltage-sensitive hemicyanine dyes
Yan P, Acker C, Zhou W, Lee P, Bollensdorff C, Negrean A, Lotti J, Sacconi L, Antic S, Kohl P, Mansvelder H, Pavone F, Loew L. Palette of fluorinated voltage-sensitive hemicyanine dyes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 20443-20448. PMID: 23169660, PMCID: PMC3528613, DOI: 10.1073/pnas.1214850109.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBiophysical PhenomenaBrainCalcium SignalingCarbocyaninesColoring AgentsDendritic SpinesFemaleFluorescent DyesFluorineGuinea PigsHeartIn Vitro TechniquesMiceMicroscopy, Fluorescence, MultiphotonMolecular StructureOptical PhenomenaPurkinje CellsRatsRats, WistarSpectrophotometryConceptsNew voltage-sensitive dyesVoltage-sensitive dyeOne-photon excitation spectrumOptical recordingTwo-photon excitationAvailable optical technologiesOptical technologiesExcitation spectraEmission spectraExcitation wavelengthRed shiftExcitation rangeExperimental requirementsSingle dendritic spinesHemicyanine dyesFluorine atomsSpectraDendritic spinesChromophoreWavelengthExcitationAtomsVoltageExperimental preparationEmissionDopaminergic Regulation of Dendritic Calcium: Fast Multisite Calcium Imaging
Zhou W, Oikonomou K, Short S, Antic S. Dopaminergic Regulation of Dendritic Calcium: Fast Multisite Calcium Imaging. Methods In Molecular Biology 2012, 964: 123-138. PMID: 23296782, DOI: 10.1007/978-1-62703-251-3_9.Peer-Reviewed Original ResearchConceptsDendritic calcium transientsCalcium transientsDendritic treeNormal cortical functionIndividual cortical neuronsElaborate dendritic treesNeocortical pyramidal neuronsSomatic action potentialsDendritic calciumGlutamatergic inputsPyramidal neuronsDopaminergic drugsDopaminergic regulationCortical neuronsDopaminergic modulationCortical functionExogenous dopamineSynaptic integrationAction potentialsCalcium imagingDopamine toneDendritic compartmentsDopaminergic signalsDendritic branchesCalcium fluctuationsPhysiological Properties of Human Fetal Cortex In Vitro
Moore A, Zhou W, Jakovcevski I, Zecevic N, Antic S. Physiological Properties of Human Fetal Cortex In Vitro. Neuromethods 2012, 73: 125-158. DOI: 10.1007/978-1-62703-020-5_3.Peer-Reviewed Original ResearchHuman fetal tissuesAnimal modelsWhole-cell patch-clamp recordingsFetal tissuesBrain developmentHuman cortical neuronsElectrical activityHuman fetal cortexAcute brain slicesSpontaneous electrical activityHuman cerebral cortexPatch-clamp recordingsNeuronal membrane excitabilityHuman cortical developmentNormal brain developmentEvolutionary changeBiophysical membrane propertiesCellular divisionCerebral cortexFetal cortexCortical neuronsBrain slicesCortical developmentPsychiatric diseasesFunctional maturationRapid dopaminergic and GABAergic modulation of calcium and voltage transients in dendrites of prefrontal cortex pyramidal neurons
Zhou W, Antic S. Rapid dopaminergic and GABAergic modulation of calcium and voltage transients in dendrites of prefrontal cortex pyramidal neurons. The Journal Of Physiology 2012, 590: 3891-3911. PMID: 22641784, PMCID: PMC3476639, DOI: 10.1113/jphysiol.2011.227157.Peer-Reviewed Original ResearchConceptsDendritic calcium transientsAction potentialsDopamine effectsSame dendriteCalcium transientsPrefrontal cortex pyramidal neuronsT-type calcium channel blockersDendritic calcium influxCortex pyramidal neuronsCalcium channel blockersMidbrain dopaminergic neuronsCalcium-sensitive dyeAP backpropagationDopaminergic stimulationGABAergic modulationPyramidal neuronsDopaminergic neuronsPulses of GABAD1 antagonistDopamine applicationDopaminergic suppressionDopaminergic inputSomatic excitabilityD1 agonistChannel blockers
2011
Spontaneous Electrical Activity in the Human Fetal Cortex In Vitro
Moore A, Zhou W, Jakovcevski I, Zecevic N, Antic S. Spontaneous Electrical Activity in the Human Fetal Cortex In Vitro. Journal Of Neuroscience 2011, 31: 2391-2398. PMID: 21325506, PMCID: PMC3564513, DOI: 10.1523/jneurosci.3886-10.2011.Peer-Reviewed Original ResearchConceptsAction potential firingSpontaneous electrical activitySP neuronsPlateau depolarizationsHuman fetal neuronsElectrical activityHuman fetal cortexFunctional synaptic contactsHuman cerebral cortexSpontaneous network activitySlow-wave sleepHuman postmortem tissueSlow oscillatory patternsGABA ionotropic receptorsHuman subplate neuronsFetal neuronsSubplate neuronsCerebral cortexGestational weeksSynaptic contactsFetal cortexPostsynaptic potentialsCortical neuronsAdult neocortexSynaptic inputs
2010
Brief dopaminergic stimulations produce transient physiological changes in prefrontal pyramidal neurons
Moore A, Zhou W, Potapenko E, Kim E, Antic S. Brief dopaminergic stimulations produce transient physiological changes in prefrontal pyramidal neurons. Brain Research 2010, 1370: 1-15. PMID: 21059342, PMCID: PMC3019254, DOI: 10.1016/j.brainres.2010.10.111.Peer-Reviewed Original ResearchConceptsPyramidal neuronsMidbrain dopaminergic neuronsPrefrontal cortexDopaminergic neuronsLayer 5 pyramidal neuronsRat medial prefrontal cortexAction potential firing ratePFC pyramidal neuronsPrefrontal pyramidal neuronsD1-like receptorsDirect synaptic contactsAction potential firingMedial prefrontal cortexDose-dependent responseDopaminergic stimulationIontophoretic pulsesCortical excitabilitySynaptic contactsDopaminergic agonistsSevere depressionPyramidal cellsTransient physiological changesBrain slicesAction potentialsCell bodiesThe decade of the dendritic NMDA spike
Antic S, Zhou W, Moore A, Short S, Ikonomu K. The decade of the dendritic NMDA spike. Journal Of Neuroscience Research 2010, 88: 2991-3001. PMID: 20544831, PMCID: PMC5643072, DOI: 10.1002/jnr.22444.Peer-Reviewed Original ResearchConceptsDendritic NMDA spikesNMDA spikesPyramidal cellsPlateau potentialsActive synaptic inputsApical tuft dendritesGlutamatergic synaptic contactsThick apical dendritesLong-term synaptic modificationsNeuronal cell bodiesCortical pyramidal cellsSlow-wave sleepCortical UP statesDendritic regenerative potentialsCortical information processingBasal dendritesPyramidal neuronsApical dendritesSynaptic contactsApical trunkTuft dendritesSynaptic inputsProximal segmentThin dendritesAwake animals
2008
Dynamics of action potential backpropagation in basal dendrites of prefrontal cortical pyramidal neurons
Zhou W, Yan P, Wuskell J, Loew L, Antic S. Dynamics of action potential backpropagation in basal dendrites of prefrontal cortical pyramidal neurons. European Journal Of Neuroscience 2008, 27: 923-936. PMID: 18279369, PMCID: PMC2715167, DOI: 10.1111/j.1460-9568.2008.06075.x.Peer-Reviewed Original ResearchConceptsBasilar dendritic treeBasal dendritesRat prefrontal cortexPyramidal neuronsAction potentialsDendritic treePrefrontal cortical pyramidal neuronsLayer 5 pyramidal neuronsPrefrontal cortexSodium action potentialsBackpropagating action potentialsCortical pyramidal neuronsAction potential backpropagationNeocortical pyramidal neuronsSomatic action potentialsCortical pyramidal cellsDistal dendritic segmentsHigh-frequency burstsVoltage-sensitive dye imaging techniqueAP backpropagationSynaptic contactsIndividual dendritic branchesPyramidal cellsNeuronal outputCalcium transients
2007
Voltage and calcium transients in basal dendrites of the rat prefrontal cortex
Milojkovic B, Zhou W, Antic S. Voltage and calcium transients in basal dendrites of the rat prefrontal cortex. The Journal Of Physiology 2007, 585: 447-468. PMID: 17932150, PMCID: PMC2375496, DOI: 10.1113/jphysiol.2007.142315.Peer-Reviewed Original ResearchConceptsDendritic plateau potentialsCalcium transientsPlateau potentialsBasal dendritesDendritic branchesSmall-amplitude depolarizationThin basal dendritesDendritic calcium transientsGlutamatergic synaptic inputsDendritic calcium dynamicsRat prefrontal cortexHigher cortical functionsThin dendritic branchesNeocortical pyramidal cellsSame dendritic branchCalcium distributionDendritic excitabilitySynaptic contactsIndividual dendritic branchesExcitatory inputsPyramidal cellsCortical functionSynaptic inputsSame dendriteSynaptic integrationIntracellular long-wavelength voltage-sensitive dyes for studying the dynamics of action potentials in axons and thin dendrites
Zhou W, Yan P, Wuskell J, Loew L, Antic S. Intracellular long-wavelength voltage-sensitive dyes for studying the dynamics of action potentials in axons and thin dendrites. Journal Of Neuroscience Methods 2007, 164: 225-239. PMID: 17560661, PMCID: PMC2001318, DOI: 10.1016/j.jneumeth.2007.05.002.Peer-Reviewed Original Research