2022
Hippocampal acetylcholine modulates stress-related behaviors independent of specific cholinergic inputs
Mineur YS, Mose TN, Vanopdenbosch L, Etherington IM, Ogbejesi C, Islam A, Pineda CM, Crouse RB, Zhou W, Thompson DC, Bentham MP, Picciotto MR. Hippocampal acetylcholine modulates stress-related behaviors independent of specific cholinergic inputs. Molecular Psychiatry 2022, 27: 1829-1838. PMID: 34997190, PMCID: PMC9106825, DOI: 10.1038/s41380-021-01404-7.Peer-Reviewed Original ResearchConceptsStress-related behaviorsCholinergic inputMedial septum/diagonal bandBehavioral effectsBrain ACh levelsChAT-positive neuronsSelective chemogenetic activationMuscarinic ACh receptorsDepression-like symptomsSignificant behavioral effectsHippocampal acetylcholineMaladaptive behavioral responsesAntidepressant effectsCholinergic neuronsACh releaseChemogenetic activationChemogenetic inhibitionCholinergic antagonistsLocal infusionAcetylcholine levelsACh levelsDiagonal bandACh receptorsHippocampal neuronsPharmacological approaches
2018
Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons
Li D, Musante V, Zhou W, Picciotto MR, Nairn AC. Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons. Journal Of Biological Chemistry 2018, 293: 11179-11194. PMID: 29802198, PMCID: PMC6052221, DOI: 10.1074/jbc.ra117.001519.Peer-Reviewed Original ResearchConceptsSerine/threonine phosphatase PP2AStriatin-interacting phosphataseRNA knockdown approachB subunitSTRIPAK complexPhosphatase PP2AProtein phosphataseMultiprotein complexesKnockdown approachStriatin familyMutant constructsStriatal neuronal culturesPP2ANeuronal developmentPrimary striatal neuronal culturesDendritic phenotypeKnockdown modelSynapse formationSubunitsSpine developmentSelective roleReduced expressionNeuron maturationNeuronal culturesStriatal neurons
2015
Antidepressant-like effects of guanfacine and sex-specific differences in effects on c-fos immunoreactivity and paired-pulse ratio in male and female mice
Mineur YS, Bentham MP, Zhou WL, Plantenga ME, McKee SA, Picciotto MR. Antidepressant-like effects of guanfacine and sex-specific differences in effects on c-fos immunoreactivity and paired-pulse ratio in male and female mice. Psychopharmacology 2015, 232: 3539-3549. PMID: 26146014, PMCID: PMC4561580, DOI: 10.1007/s00213-015-4001-3.Peer-Reviewed Original ResearchConceptsAntidepressant-like effectsPaired-pulse ratioC-Fos immunoreactivityPrefrontal cortexSwim testBrain areasRobust antidepressant-like effectsBrain regionsSex differencesMale C57BL/6J miceDepression-like behaviorEffects of guanfacineAcetylcholinesterase inhibitor physostigmineLight/dark boxBaseline sex differencesC-fos expressionDepression-like stateCritical brain regionsDifferent brain areasSex-specific changesAntidepressant efficacyCholinergic controlInhibitor physostigmineC57BL/6J miceAgonist guanfacine
2012
Palette of fluorinated voltage-sensitive hemicyanine dyes
Yan P, Acker C, Zhou W, Lee P, Bollensdorff C, Negrean A, Lotti J, Sacconi L, Antic S, Kohl P, Mansvelder H, Pavone F, Loew L. Palette of fluorinated voltage-sensitive hemicyanine dyes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 20443-20448. PMID: 23169660, PMCID: PMC3528613, DOI: 10.1073/pnas.1214850109.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBiophysical PhenomenaBrainCalcium SignalingCarbocyaninesColoring AgentsDendritic SpinesFemaleFluorescent DyesFluorineGuinea PigsHeartIn Vitro TechniquesMiceMicroscopy, Fluorescence, MultiphotonMolecular StructureOptical PhenomenaPurkinje CellsRatsRats, WistarSpectrophotometryConceptsNew voltage-sensitive dyesVoltage-sensitive dyeOne-photon excitation spectrumOptical recordingTwo-photon excitationAvailable optical technologiesOptical technologiesExcitation spectraEmission spectraExcitation wavelengthRed shiftExcitation rangeExperimental requirementsSingle dendritic spinesHemicyanine dyesFluorine atomsSpectraDendritic spinesChromophoreWavelengthExcitationAtomsVoltageExperimental preparationEmission
2005
A novel artemisinin derivative, 3-(12-β-artemisininoxy) phenoxyl succinic acid (SM735), mediates immunosuppressive effects in vitro and in vivo
Zhou W, Wu J, Wu Q, Wang J, Zhou Y, Zhou R, He P, Li X, Yang Y, Zhang Y, Li Y, Zuo J. A novel artemisinin derivative, 3-(12-β-artemisininoxy) phenoxyl succinic acid (SM735), mediates immunosuppressive effects in vitro and in vivo. Acta Pharmacologica Sinica 2005, 26: 1352-1358. PMID: 16225758, DOI: 10.1111/j.1745-7254.2005.00232.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Inflammatory Agents, Non-SteroidalArtemisininsCell ProliferationCells, CulturedCytokinesDose-Response Relationship, DrugHemolysisHypersensitivity, DelayedImmunosuppressive AgentsInhibitory Concentration 50Interferon-gammaInterleukin-12Lymphocyte Culture Test, MixedMaleMiceMice, Inbred BALB CMice, Inbred C57BLSuccinatesConceptsDelayed-type hypersensitivityMixed lymphocyte reactionProliferation of splenocytesSheep red blood cellsImmunosuppressive agentsCytokine productionT-cell-mediated delayed-type hypersensitivityImmunosuppressive activityVivo immune activityProinflammatory cytokine productionIL-2 secretionNovel artemisinin derivativesEnzyme-linked immunosorbentDose-dependent mannerStrong immunosuppressive activityPotential immunosuppressive agentsRed blood cellsLymphocyte reactionImmunosuppressive effectsQuantitative hemolysisImmune activityMouse modelArtemisinin derivativesConA stimulationBlood cells(5R)-5-hydroxytriptolide (LLDT-8), a novel triptolide analog mediates immunosuppressive effects in vitro and in vivo
Zhou R, Zhang F, He P, Zhou W, Wu Q, Xu J, Zhou Y, Tang W, Li X, Yang Y, Li Y, Zuo J. (5R)-5-hydroxytriptolide (LLDT-8), a novel triptolide analog mediates immunosuppressive effects in vitro and in vivo. International Immunopharmacology 2005, 5: 1895-1903. PMID: 16275624, DOI: 10.1016/j.intimp.2005.06.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesCell ProliferationCell SurvivalCells, CulturedCytokinesDinitrofluorobenzeneDisease Models, AnimalDiterpenesDrugs, Chinese HerbalFemaleHypersensitivity, DelayedImmunosuppressive AgentsLymphocyte ActivationLymphocyte Culture Test, MixedMaleMiceMice, Inbred BALB CSheepSpleenT-LymphocytesConceptsLLDT-8Immunosuppressive activityBLAB/c miceType hypersensitivity reactionSheep red blood cellsTh1-type cytokinesHumoral immune responseProliferation of splenocytesImmune-related diseasesHigh immunosuppressive activityPotential therapeutic agentNovel triptolide analogRed blood cellsLymphocyte reactionHypersensitivity reactionsImmunosuppressive effectsInflammatory cytokinesLow acute toxicityC miceImmune responseType cytokinesTriptolide analoguesAntibody productionTherapeutic agentsBlood cellsSynthesis and Immunosuppressive Activity of New Artemisinin Derivatives. 1. [12(β or α)-Dihydroartemisininoxy]phen(ox)yl Aliphatic Acids and Esters
Yang Z, Zhou W, Sui Y, Wang J, Wu J, Zhou Y, Zhang Y, He P, Han J, Tang W, Li Y, Zuo J. Synthesis and Immunosuppressive Activity of New Artemisinin Derivatives. 1. [12(β or α)-Dihydroartemisininoxy]phen(ox)yl Aliphatic Acids and Esters. Journal Of Medicinal Chemistry 2005, 48: 4608-4617. PMID: 15999998, DOI: 10.1021/jm048979c.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationArtemisininsB-LymphocytesCell ProliferationConcanavalin ADinitrofluorobenzeneErythrocytesEstersFatty AcidsFemaleHypersensitivity, DelayedImmunosuppressive AgentsIn Vitro TechniquesLipopolysaccharidesMaleMiceMice, Inbred BALB CMitogensSesquiterpenesSheepStructure-Activity RelationshipT-LymphocytesConceptsB cell proliferationSheep red blood cellsT cell proliferationCell proliferationImmunosuppressive activityDelayed-type hypersensitivity reactionCytotoxicity of lymphocytesNew artemisinin derivativesPotential immunosuppressive agentsRed blood cellsImmunosuppressive agentsHypersensitivity reactionsT cellsInhibition activityArtemisinin derivativesAntibody productionCyclosporin ADihydroartemisinin derivativesBlood cellsInhibitory effectHigh efficacyLipopolysaccharideConcanavalin AProliferationHighest inhibition activityInhibition of S-Adenosyl-l-homocysteine Hydrolase Induces Immunosuppression
Wu Q, Fu Y, Zhou W, Wang J, Feng Y, Liu J, Xu J, He P, Zhou R, Tang W, Wang G, Zhou Y, Yang Y, Ding J, Li X, Chen X, Yuan C, Lawson B, Zuo J. Inhibition of S-Adenosyl-l-homocysteine Hydrolase Induces Immunosuppression. Journal Of Pharmacology And Experimental Therapeutics 2005, 313: 705-711. PMID: 15640397, DOI: 10.1124/jpet.104.080416.Peer-Reviewed Original ResearchMeSH KeywordsAdenosylhomocysteinaseAnimalsAntigens, CDB7-1 AntigenB7-2 AntigenCell SurvivalDose-Response Relationship, DrugDown-RegulationEnzyme ActivationEnzyme InhibitorsFemaleGrowth InhibitorsImmunosuppressive AgentsLymphocyte Culture Test, MixedMaleMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C57BLConceptsTHP-1 cellsImmunosuppressive effectsTumor necrosis factor-alpha productionDelayed-type hypersensitivity reactionS-adenosyl-L-homocysteine hydrolaseNecrosis factor-alpha productionLevels of CD80IL-12 productionMixed lymphocyte reactionT cell inhibitionHuman monocytic THP-1 cellsThioglycollate-stimulated peritoneal macrophagesT cell functionT cell proliferationImmunosuppressive therapeutic agentsT cell costimulationMonocytic THP-1 cellsDose-dependent mannerImmunologic effectsIL-12Lymphocyte reactionHypersensitivity reactionsCytokine outputMacrophage activationType III inhibitor
2002
Low dose of resveratrol enhanced immune response of mice.
Feng Y, Zhou W, Wu Q, Li X, Zhao W, Zou J. Low dose of resveratrol enhanced immune response of mice. Acta Pharmacologica Sinica 2002, 23: 893-7. PMID: 12370094.Peer-Reviewed Original ResearchConceptsCytokine productionLymphocyte subtypesImmune responseLow doseMHC-II molecule expressionCell-mediated immune responsesLow-dose resveratrolType hypersensitivity responseImmune modulating effectsTh1 cytokine productionIL-10 productionIFN-gamma productionStaphylococcus aureus CowanResponse of miceIL-2 productionProliferation of lymphocytesEar swellingIL-12Hypersensitivity responseLymphocyte proliferationMacrophage percentageMolecule expressionT lymphocytesResveratrol treatmentEthanol consumption