2018
Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast
Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, Gilbert WV. Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast. PLOS Biology 2018, 16: e2005903. PMID: 30208026, PMCID: PMC6135351, DOI: 10.1371/journal.pbio.2005903.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCodon, InitiatorConserved SequenceGene Expression Regulation, FungalHeLa CellsHumansPeptide Chain Elongation, TranslationalPeptide Chain Termination, TranslationalProtein BiosynthesisRibosomal ProteinsRibosomesRNA, RibosomalRNA, TransferSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsRibosome-binding proteinsRibosome-associated proteinsCodon-specific changesCoiled-coil domainQuantitative mass spectrometryGTPase activation centerRibosome-binding activityMost tRNAsRibosome profilingGene regulationMost genesCellular homeostasisTranslation defectsRibosomal RNATranslational recoveryComplete complementStart codonStop codonLso2Protein synthesisOpen reading frame 2ProteinImportant functionsCodonYeast
2017
Translation initiation factor eIF4G1 preferentially binds yeast transcript leaders containing conserved oligo-uridine motifs
Zinshteyn B, Rojas-Duran MF, Gilbert WV. Translation initiation factor eIF4G1 preferentially binds yeast transcript leaders containing conserved oligo-uridine motifs. RNA 2017, 23: 1365-1375. PMID: 28546148, PMCID: PMC5558906, DOI: 10.1261/rna.062059.117.Peer-Reviewed Original ResearchConceptsTranscript leaderEIF4GTranslational controlInitiation factorsRNA sequencesSpecific mRNAsEukaryotic initiation factor 4GRNA-binding preferencesGeneral initiation factorsIntrinsic sequence preferencesImportant cellular functionsCellular stress responseTranslation initiation factorTranslation of mRNAsOrganismal developmentYeast genesRibosome profilingContext-dependent differencesTranslational regulationCellular functionsPreferential translationYeast speciesKey mRNAsGene expressionSequence preference