2015
Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9
Abrahimi P, Chang WG, Kluger MS, Qyang Y, Tellides G, Saltzman WM, Pober JS. Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9. Circulation Research 2015, 117: 121-128. PMID: 25940550, PMCID: PMC4490936, DOI: 10.1161/circresaha.117.306290.Peer-Reviewed Original ResearchAnimalsCD4-Positive T-LymphocytesCell SeparationCells, CulturedCRISPR-Cas SystemsEndothelial Progenitor CellsFemaleFetal BloodGene DeletionGene Knockout TechniquesGenes, MHC Class IIGenetic VectorsHLA-DR AntigensHumansIntracellular Signaling Peptides and ProteinsLentivirusLymphocyte ActivationLymphocyte Culture Test, MixedMiceMice, SCIDNuclear ProteinsPrimary Cell CultureProteinsTetracyclineTrans-ActivatorsVesicular Transport Proteins
2004
Impact of E1a Modifications on Tumor-Selective Adenoviral Replication and Toxicity
Sauthoff H, Pipiya T, Heitner S, Chen S, Bleck B, Reibman J, Chang W, Norman RG, Rom WN, Hay JG. Impact of E1a Modifications on Tumor-Selective Adenoviral Replication and Toxicity. Molecular Therapy 2004, 10: 749-757. PMID: 15451459, DOI: 10.1016/j.ymthe.2004.07.014.Peer-Reviewed Original ResearchConceptsS-phase inductionN-terminusNormal cellsCancer cellsE1A deletion mutantsGrowth-arrested cellsViral replicationNormal bronchial epithelial cellsNormal cell typesE1A proteinsWild-type virusWild typeBronchial epithelial cellsS phaseCell typesDeletionE1ACMV promoterEpithelial cellsReplicationAdenoviral replicationCytotoxicity profileCellsSelective viral replicationTarget cells