1984
Isolation of spectrin subunits and reassociation in vitro. Analysis by fluorescence polarization.
Yoshino H, Marchesi V. Isolation of spectrin subunits and reassociation in vitro. Analysis by fluorescence polarization. Journal Of Biological Chemistry 1984, 259: 4496-4500. PMID: 6707015, DOI: 10.1016/s0021-9258(17)43074-2.Peer-Reviewed Original ResearchConceptsFluorescence anisotropy changesFluorescence polarizationEventual dissociationGood yieldsDEAE-cellulose column chromatographyOligomeric speciesMonomer stateColumn chromatographyNeutral salt solutionsElectron microscopyAcrylamide gel electrophoresisSalt solutionLow-angle shadowingM ureaTryptophan residuesBeta subunitPure alphaSpectrinSubdomain structureSpectrin moleculesSubunitsLow concentrationsErythrocyte spectrinSpectrin subunitsGel electrophoresis
1977
Phosphorylation in membranes of intact human erythrocytes.
Shapiro D, Marchesi V. Phosphorylation in membranes of intact human erythrocytes. Journal Of Biological Chemistry 1977, 252: 508-517. PMID: 188816, DOI: 10.1016/s0021-9258(17)32746-1.Peer-Reviewed Original ResearchConceptsIntact human erythrocytesStudy of phosphorylationPattern of phosphorylationHuman erythrocytesIntact cell systemMembrane proteinsLipid phosphorylationIntracellular portionIntact cellsPolyacrylamide gel electrophoresisMembrane phosphorylationPhosphorylationMembrane sialoglycoproteinGlycophorin AGlycophorin A.Gel electrophoresisPhosphate exchangeCell system