1985
The binding of vimentin to human erythrocyte membranes: a model system for the study of intermediate filament-membrane interactions.
Georgatos S, Marchesi V. The binding of vimentin to human erythrocyte membranes: a model system for the study of intermediate filament-membrane interactions. Journal Of Cell Biology 1985, 100: 1955-1961. PMID: 3158664, PMCID: PMC2113610, DOI: 10.1083/jcb.100.6.1955.Peer-Reviewed Original ResearchConceptsBinding of vimentinProtein 4.1Membrane vesiclesAnti-ankyrin antibodiesErythrocyte membrane skeletonHuman erythrocyte membrane vesiclesIntermediate filament proteinsErythrocyte membrane vesiclesMajor attachment siteMembrane skeletonPlasma membraneBinding functionsAnkyrinFilament proteinsCytoplasmic fragmentsBand 4.5Glycophorin ASpectrinVesiclesAttachment sitesHuman erythrocyte membranesBand 3
1984
Isolation of spectrin subunits and reassociation in vitro. Analysis by fluorescence polarization.
Yoshino H, Marchesi V. Isolation of spectrin subunits and reassociation in vitro. Analysis by fluorescence polarization. Journal Of Biological Chemistry 1984, 259: 4496-4500. PMID: 6707015, DOI: 10.1016/s0021-9258(17)43074-2.Peer-Reviewed Original ResearchConceptsFluorescence anisotropy changesFluorescence polarizationEventual dissociationGood yieldsDEAE-cellulose column chromatographyOligomeric speciesMonomer stateColumn chromatographyNeutral salt solutionsElectron microscopyAcrylamide gel electrophoresisSalt solutionLow-angle shadowingM ureaTryptophan residuesBeta subunitPure alphaSpectrinSubdomain structureSpectrin moleculesSubunitsLow concentrationsErythrocyte spectrinSpectrin subunitsGel electrophoresis