2017
Mitochondrial Targeted Catalase Protects Against High-Fat Diet-Induced Muscle Insulin Resistance by Decreasing Intramuscular Lipid Accumulation
Lee HY, Lee JS, Alves T, Ladiges W, Rabinovitch PS, Jurczak MJ, Choi CS, Shulman GI, Samuel VT. Mitochondrial Targeted Catalase Protects Against High-Fat Diet-Induced Muscle Insulin Resistance by Decreasing Intramuscular Lipid Accumulation. Diabetes 2017, 66: db161334. PMID: 28476930, PMCID: PMC5521865, DOI: 10.2337/db16-1334.Peer-Reviewed Original ResearchConceptsHigh-fat dietMuscle insulin resistanceAcute lipid infusionInsulin resistanceRegular chowLipid infusionMCAT miceInsulin actionLipid-induced insulin resistanceDiet-induced insulin resistanceReactive oxygen speciesHyperinsulinemic-euglycemic clampWild-type miceMuscle fat oxidationIntramuscular lipid accumulationROS productionAcute infusionHFD-fedWT miceImpaired insulinPKCθ activationFat oxidationLipid emulsionMuscle insulinMice
2010
Deletion of the α-Arrestin Protein Txnip in Mice Promotes Adiposity and Adipogenesis While Preserving Insulin Sensitivity
Chutkow WA, Birkenfeld AL, Brown JD, Lee HY, Frederick DW, Yoshioka J, Patwari P, Kursawe R, Cushman SW, Plutzky J, Shulman GI, Samuel VT, Lee RT. Deletion of the α-Arrestin Protein Txnip in Mice Promotes Adiposity and Adipogenesis While Preserving Insulin Sensitivity. Diabetes 2010, 59: 1424-1434. PMID: 20299477, PMCID: PMC2874703, DOI: 10.2337/db09-1212.Peer-Reviewed Original ResearchConceptsTxnip knockout miceInsulin resistanceInsulin sensitivityKnockout miceInsulin responsivenessTXNIP expressionSkeletal muscleWild-type littermate control miceStandard chow dietType 2 diabetes pathogenesisHigh-fat dietHigh-fat feedingLittermate control miceGene-deleted miceInhibits glucose uptakeControl miceChow dietAdipose massMore insulinCaloric excessFat massDiabetes pathogenesisMouse embryonic fibroblastsRegulator of adipogenesisPPARgamma expression
2001
Disruption of Sur2-containing KATP channels enhances insulin-stimulated glucose uptake in skeletal muscle
Chutkow W, Samuel V, Hansen P, Pu J, Valdivia C, Makielski J, Burant C. Disruption of Sur2-containing KATP channels enhances insulin-stimulated glucose uptake in skeletal muscle. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 11760-11764. PMID: 11562480, PMCID: PMC58803, DOI: 10.1073/pnas.201390398.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsATP-Binding Cassette TransportersBiological TransportBlood GlucoseDeoxyglucoseExonsGlucoseGlucose Clamp TechniqueGlucose Tolerance TestGlucose Transporter Type 4InsulinIntronsMiceMice, KnockoutMonosaccharide Transport ProteinsMuscle ProteinsMuscle, SkeletalPolymerase Chain ReactionPotassium ChannelsPotassium Channels, Inwardly RectifyingReceptors, DrugRNA, MessengerSignal TransductionSodium-Potassium-Exchanging ATPaseSulfonylurea ReceptorsTriglyceridesWeight GainConceptsSkeletal muscleInsulin-stimulated glucose transportGene-targeting strategiesGlucose uptake mechanismsInsulin-stimulated glucose uptakeHomozygous null miceRegulatory subunitInsertional mutagenesisWild typeEnhanced glucose useProtection of tissuesDiverse arrayGlucose transportChannel activityUptake mechanismNull miceATP-sensitive potassium channelsPotassium channelsGlucose uptakeMembrane excitabilityFuture therapeutic approachesWild-type littermatesTarget blood glucose levelsInsulin actionPhysiologic function