2023
Organ function is preserved despite reorganization of niche architecture in the hair follicle
Wei H, Du S, Parksong J, Pasolli H, Matte-Martone C, Regot S, Gonzalez L, Xin T, Greco V. Organ function is preserved despite reorganization of niche architecture in the hair follicle. Cell Stem Cell 2023, 30: 962-972.e6. PMID: 37419106, PMCID: PMC10362479, DOI: 10.1016/j.stem.2023.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationEpitheliumHairHair FollicleMiceTransforming Growth Factor betaConceptsNiche architectureDermal papilla fibroblastsDifferentiated lineagesHair follicle growthStereotypic architectureMultipotent progenitorsEpithelial progenitorsFunctional importanceNicheStem cellsFibroblast nicheProgenitorsPowerful modelIntravital imagingDermal papillaFibroblastsHair folliclesFollicle growthOrgan functionLineagesDifferentiationCrosstalkHairProliferationCellsLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2022
Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer
Cockburn K, Annusver K, Gonzalez D, Ganesan S, May D, Mesa K, Kawaguchi K, Kasper M, Greco V. Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer. Nature Cell Biology 2022, 24: 1692-1700. PMID: 36357619, PMCID: PMC9729105, DOI: 10.1038/s41556-022-01021-8.Peer-Reviewed Original ResearchConceptsCell cycle exitCycle exitStem cell layerUndifferentiated stem cellsStem cell compartmentDaughter cellsDifferentiated populationsDifferentiation genesTranscriptional changesCell divisionMultiple progenitorsStemness genesCells transitStem cellsCell compartmentCell layerCell poolDifferentiationRegenerative tissueGenesSkin epidermisGradual differentiationMulti-day processesCells
2018
Flexible fate determination ensures robust differentiation in the hair follicle
Xin T, Gonzalez D, Rompolas P, Greco V. Flexible fate determination ensures robust differentiation in the hair follicle. Nature Cell Biology 2018, 20: 1361-1369. PMID: 30420661, PMCID: PMC6314017, DOI: 10.1038/s41556-018-0232-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineageHair FollicleHomeostasisMice, KnockoutMice, TransgenicSingle-Cell AnalysisStem CellsConceptsSingle-cell levelStem cellsStem cell differentiationGerm stem cellsTissue architectureMultiple cell typesFate determinationDetermination mechanismTissue homeostasisSame stem cellsCommon progenitorDifferentiation outcomesDifferentiation stimuliDifferentiation lineageCell differentiationCell typesNormal differentiationWnt activationHair folliclesUnanticipated flexibilityDifferentiationRobust differentiationProgenitorsCellsUninjured conditionHomeostatic Epidermal Stem Cell Self-Renewal Is Driven by Local Differentiation
Mesa KR, Kawaguchi K, Cockburn K, Gonzalez D, Boucher J, Xin T, Klein AM, Greco V. Homeostatic Epidermal Stem Cell Self-Renewal Is Driven by Local Differentiation. Cell Stem Cell 2018, 23: 677-686.e4. PMID: 30269903, PMCID: PMC6214709, DOI: 10.1016/j.stem.2018.09.005.Peer-Reviewed Original Research
2016
Spatiotemporal coordination of stem cell commitment during epidermal homeostasis
Rompolas P, Mesa KR, Kawaguchi K, Park S, Gonzalez D, Brown S, Boucher J, Klein AM, Greco V. Spatiotemporal coordination of stem cell commitment during epidermal homeostasis. Science 2016, 352: 1471-1474. PMID: 27229141, PMCID: PMC4958018, DOI: 10.1126/science.aaf7012.Peer-Reviewed Original Research
2015
Intravital imaging of hair follicle regeneration in the mouse
Pineda CM, Park S, Mesa KR, Wolfel M, Gonzalez DG, Haberman AM, Rompolas P, Greco V. Intravital imaging of hair follicle regeneration in the mouse. Nature Protocols 2015, 10: 1116-1130. PMID: 26110716, PMCID: PMC4632978, DOI: 10.1038/nprot.2015.070.Peer-Reviewed Original Research
2014
β-Catenin Activation Regulates Tissue Growth Non–Cell Autonomously in the Hair Stem Cell Niche
Deschene ER, Myung P, Rompolas P, Zito G, Sun TY, Taketo MM, Saotome I, Greco V. β-Catenin Activation Regulates Tissue Growth Non–Cell Autonomously in the Hair Stem Cell Niche. Science 2014, 343: 1353-1356. PMID: 24653033, PMCID: PMC4096864, DOI: 10.1126/science.1248373.Peer-Reviewed Original ResearchConceptsWild-type cellsWnt/β-catenin signalingΒ-catenin signalingΒ-catenin activationMouse hair follicle stem cellsΒ-cateninStem cell nicheHair follicle stem cellsFollicle stem cellsNiche signalsMutant cellsCell divisionCell nicheCoordinated regenerationHair growthWnt ligandsCellular displacementCell behaviorStem cellsHair regenerationTissue growthSignalingCellsTissue regenerationActivation
2013
Spatial organization within a niche as a determinant of stem-cell fate
Rompolas P, Mesa KR, Greco V. Spatial organization within a niche as a determinant of stem-cell fate. Nature 2013, 502: 513-518. PMID: 24097351, PMCID: PMC3895444, DOI: 10.1038/nature12602.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell DivisionCell LineageEpithelial CellsHair FollicleMaleMiceRegenerationStem Cell NicheStem CellsConceptsStem cell fateHair follicle nicheStem cell lineagesStem cellsStem cell nicheHair follicle stem cellsStem cell compartmentFollicle stem cellsFate determinationGenetic lineagesDifferentiated fateAdult tissuesNiche locationsMammalian tissuesNicheHair regenerationLineagesSpatial organizationEpithelial cellsFateCellsHair growthRegenerationLive miceTissue