2024
Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation
Xin T, Gallini S, Wei H, Gonzalez D, Matte-Martone C, Machida H, Fujiwara H, Pasolli H, Suozzi K, Gonzalez L, Regot S, Greco V. Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation. Nature Cell Biology 2024, 26: 859-867. PMID: 38689013, PMCID: PMC11519783, DOI: 10.1038/s41556-024-01413-y.Peer-Reviewed Original ResearchERK signalingStem cellsSquamous cell carcinomaHair folliclesOncogenic KRAS mutationsCell carcinomaKRAS mutationsSustained ERK activationERK signaling dynamicsOncogenic mutationsOncogenic KRASERK activationStem cell behaviorIntravital imagingAbnormal cell divisionModulates specific featuresKrasG12DTissue deformationSpatiotemporally specific mannerSustained ERK signalingMutationsLiving miceFolliclesTissue disruptionSingle-cell level
2023
Organ function is preserved despite reorganization of niche architecture in the hair follicle
Wei H, Du S, Parksong J, Pasolli H, Matte-Martone C, Regot S, Gonzalez L, Xin T, Greco V. Organ function is preserved despite reorganization of niche architecture in the hair follicle. Cell Stem Cell 2023, 30: 962-972.e6. PMID: 37419106, PMCID: PMC10362479, DOI: 10.1016/j.stem.2023.06.003.Peer-Reviewed Original ResearchConceptsNiche architectureDermal papilla fibroblastsDifferentiated lineagesHair follicle growthStereotypic architectureMultipotent progenitorsEpithelial progenitorsFunctional importanceNicheStem cellsFibroblast nicheProgenitorsPowerful modelIntravital imagingDermal papillaFibroblastsHair folliclesFollicle growthOrgan functionLineagesDifferentiationCrosstalkHairProliferationCellsCell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2023, 222: e202302095. PMID: 37102999, PMCID: PMC10140546, DOI: 10.1083/jcb.202302095.Peer-Reviewed Original ResearchLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2022
Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer
Cockburn K, Annusver K, Gonzalez D, Ganesan S, May D, Mesa K, Kawaguchi K, Kasper M, Greco V. Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer. Nature Cell Biology 2022, 24: 1692-1700. PMID: 36357619, PMCID: PMC9729105, DOI: 10.1038/s41556-022-01021-8.Peer-Reviewed Original ResearchConceptsCell cycle exitCycle exitStem cell layerUndifferentiated stem cellsStem cell compartmentDaughter cellsDifferentiated populationsDifferentiation genesTranscriptional changesCell divisionMultiple progenitorsStemness genesCells transitStem cellsCell compartmentCell layerCell poolDifferentiationRegenerative tissueGenesSkin epidermisGradual differentiationMulti-day processesCells
2020
Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo
Pokrass MJ, Ryan KA, Xin T, Pielstick B, Timp W, Greco V, Regot S. Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo. Developmental Cell 2020, 55: 328-340.e5. PMID: 33091369, PMCID: PMC7658051, DOI: 10.1016/j.devcel.2020.09.013.Peer-Reviewed Original ResearchConceptsFate specificationPreimplantation developmentKinase translocation reporterMammalian preimplantation embryosInner cell massEmbryonic stem cellsSingle cellsDifferent cell typesMulticellular organismsEndogenous taggingDaughter cellsNanog proteinActive ERKNanog levelsERK activityGene expressionPreimplantation embryosCell cycleTrophectoderm cellsERK inhibitionCell typesStem cellsLive embryosCell massEmbryos
2019
Hair follicle regeneration suppresses Ras-driven oncogenic growth
Pineda CM, Gonzalez DG, Matte-Martone C, Boucher J, Lathrop E, Gallini S, Fons NR, Xin T, Tai K, Marsh E, Nguyen DX, Suozzi KC, Beronja S, Greco V. Hair follicle regeneration suppresses Ras-driven oncogenic growth. Journal Of Cell Biology 2019, 218: 3212-3222. PMID: 31488583, PMCID: PMC6781447, DOI: 10.1083/jcb.201907178.Peer-Reviewed Original ResearchConceptsHair folliclesHras mutationsOncogenic growthHair follicle stem cellsSkin hair folliclesTumor developmentFollicle stem cellsHair follicle regenerationSkin epitheliumSecondary mutationsBenign outgrowthFolliclesStem cellsTissueCertain tissuesFollicle regenerationCellsContinuous tissueWild-type neighborsDistinct mechanismsDifferent outcomesMutationsEnhanced capacityInjury
2018
Flexible fate determination ensures robust differentiation in the hair follicle
Xin T, Gonzalez D, Rompolas P, Greco V. Flexible fate determination ensures robust differentiation in the hair follicle. Nature Cell Biology 2018, 20: 1361-1369. PMID: 30420661, PMCID: PMC6314017, DOI: 10.1038/s41556-018-0232-y.Peer-Reviewed Original ResearchConceptsSingle-cell levelStem cellsStem cell differentiationGerm stem cellsTissue architectureMultiple cell typesFate determinationDetermination mechanismTissue homeostasisSame stem cellsCommon progenitorDifferentiation outcomesDifferentiation stimuliDifferentiation lineageCell differentiationCell typesNormal differentiationWnt activationHair folliclesUnanticipated flexibilityDifferentiationRobust differentiationProgenitorsCellsUninjured conditionHomeostatic Epidermal Stem Cell Self-Renewal Is Driven by Local Differentiation
Mesa KR, Kawaguchi K, Cockburn K, Gonzalez D, Boucher J, Xin T, Klein AM, Greco V. Homeostatic Epidermal Stem Cell Self-Renewal Is Driven by Local Differentiation. Cell Stem Cell 2018, 23: 677-686.e4. PMID: 30269903, PMCID: PMC6214709, DOI: 10.1016/j.stem.2018.09.005.Peer-Reviewed Original Research
2016
Hardwiring Stem Cell Communication through Tissue Structure
Xin T, Greco V, Myung P. Hardwiring Stem Cell Communication through Tissue Structure. Cell 2016, 164: 1212-1225. PMID: 26967287, PMCID: PMC4805424, DOI: 10.1016/j.cell.2016.02.041.Peer-Reviewed Original Research
2015
Intravital imaging of hair follicle regeneration in the mouse
Pineda CM, Park S, Mesa KR, Wolfel M, Gonzalez DG, Haberman AM, Rompolas P, Greco V. Intravital imaging of hair follicle regeneration in the mouse. Nature Protocols 2015, 10: 1116-1130. PMID: 26110716, PMCID: PMC4632978, DOI: 10.1038/nprot.2015.070.Peer-Reviewed Original ResearchThe Dynamic Duo: Niche/Stem Cell Interdependency
Mesa KR, Rompolas P, Greco V. The Dynamic Duo: Niche/Stem Cell Interdependency. Stem Cell Reports 2015, 4: 961-966. PMID: 26028534, PMCID: PMC4471832, DOI: 10.1016/j.stemcr.2015.05.001.Peer-Reviewed Original ResearchConceptsNormal tissue homeostasisStem cell behaviorStem cell interactionsTissue homeostasisCellular turnoverPathological settingsCell behaviorStem cellsMost tissuesFundamental processesDynamic duoCell interactionsModel systemNew cellsCell productionTissue regenerationCellsTissueLive miceOrganismsHomeostasisTremendous advancesImaging approachNovel imaging approachBasic understandingNiche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool
Mesa KR, Rompolas P, Zito G, Myung P, Sun TY, Brown S, Gonzalez DG, Blagoev KB, Haberman AM, Greco V. Niche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature 2015, 522: 94-97. PMID: 25849774, PMCID: PMC4457634, DOI: 10.1038/nature14306.Peer-Reviewed Original Research
2014
β-Catenin Activation Regulates Tissue Growth Non–Cell Autonomously in the Hair Stem Cell Niche
Deschene ER, Myung P, Rompolas P, Zito G, Sun TY, Taketo MM, Saotome I, Greco V. β-Catenin Activation Regulates Tissue Growth Non–Cell Autonomously in the Hair Stem Cell Niche. Science 2014, 343: 1353-1356. PMID: 24653033, PMCID: PMC4096864, DOI: 10.1126/science.1248373.Peer-Reviewed Original ResearchConceptsWild-type cellsWnt/β-catenin signalingΒ-catenin signalingΒ-catenin activationMouse hair follicle stem cellsΒ-cateninStem cell nicheHair follicle stem cellsFollicle stem cellsNiche signalsMutant cellsCell divisionCell nicheCoordinated regenerationHair growthWnt ligandsCellular displacementCell behaviorStem cellsHair regenerationTissue growthSignalingCellsTissue regenerationActivation
2013
Stem cell dynamics in the hair follicle niche
Rompolas P, Greco V. Stem cell dynamics in the hair follicle niche. Seminars In Cell And Developmental Biology 2013, 25: 34-42. PMID: 24361866, PMCID: PMC3988239, DOI: 10.1016/j.semcdb.2013.12.005.Peer-Reviewed Original ResearchConceptsHair follicle nicheStem cellsStem cell behaviorMouse genetic modelsNon-cellular componentsNiche microenvironmentMolecular signalsCell behaviorNicheMammalian skinHair folliclesProcess of regenerationCell dynamicsCell populationsGenetic modelsExtracellular materialCurrent understandingCellular compositionTissue regenerationCellsRegenerationNew hairAppendagesMicroenvironmentFolliclesSpatial organization within a niche as a determinant of stem-cell fate
Rompolas P, Mesa KR, Greco V. Spatial organization within a niche as a determinant of stem-cell fate. Nature 2013, 502: 513-518. PMID: 24097351, PMCID: PMC3895444, DOI: 10.1038/nature12602.Peer-Reviewed Original ResearchConceptsStem cell fateHair follicle nicheStem cell lineagesStem cellsStem cell nicheHair follicle stem cellsStem cell compartmentFollicle stem cellsFate determinationGenetic lineagesDifferentiated fateAdult tissuesNiche locationsMammalian tissuesNicheHair regenerationLineagesSpatial organizationEpithelial cellsFateCellsHair growthRegenerationLive miceTissue
2012
Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration
Rompolas P, Deschene ER, Zito G, Gonzalez DG, Saotome I, Haberman AM, Greco V. Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration. Nature 2012, 487: 496-499. PMID: 22763436, PMCID: PMC3772651, DOI: 10.1038/nature11218.Peer-Reviewed Original Research
2010
Compartmentalized organization: a common and required feature of stem cell niches?
Greco V, Guo S. Compartmentalized organization: a common and required feature of stem cell niches? Development 2010, 137: 1586-1594. PMID: 20430743, PMCID: PMC2860245, DOI: 10.1242/dev.041103.Peer-Reviewed Original ResearchConceptsStem cell nicheCell nicheHair follicle stem cell nicheFollicle stem cell nicheAdult stem cell nichesStem cellsStem cell fieldOrgan growthNicheHair regenerationSlow cyclingRecent findingsCell fieldNew growthTissue regenerationRecent studiesCellsGrowthLong-term growthRegenerationProgenyCompartmentsKey questions
2009
A Two-Step Mechanism for Stem Cell Activation during Hair Regeneration
Greco V, Chen T, Rendl M, Schober M, Pasolli HA, Stokes N, dela Cruz-Racelis J, Fuchs E. A Two-Step Mechanism for Stem Cell Activation during Hair Regeneration. Cell Stem Cell 2009, 4: 155-169. PMID: 19200804, PMCID: PMC2668200, DOI: 10.1016/j.stem.2008.12.009.Peer-Reviewed Original ResearchConceptsBMP inhibitorsHair germHG cellsStem cellsStem cell activationHair regenerationTransit-amplifying cellsBulge stem cellsDermal papillaCyclic boutsTranscriptional profilingElevated FGFPrecocious activityHair folliclesBulge cellsLate telogenWntCell clustersCell activationCellsTwo-step mechanismSmall cell clustersInitial stepRegenerationInhibitors
2007
Mice cloned from skin cells
Li J, Greco V, Guasch G, Fuchs E, Mombaerts P. Mice cloned from skin cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 2738-2743. PMID: 17299040, PMCID: PMC1815251, DOI: 10.1073/pnas.0611358104.Peer-Reviewed Original Research