2019
Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretion
2011
Renal outer medullary potassium channel knockout models reveal thick ascending limb function and dysfunction
Wang T. Renal outer medullary potassium channel knockout models reveal thick ascending limb function and dysfunction. Clinical And Experimental Nephrology 2011, 16: 49-54. PMID: 22038261, DOI: 10.1007/s10157-011-0495-0.Peer-Reviewed Original ResearchConceptsThick ascending limbIon transporter expressionRenal outer medullary potassium channelBartter's syndromeInward rectifier potassium channelPotassium channelsSmall-conductance K channelsROMK null miceMedullary thick ascending limbType II Bartter's syndromeSimilar phenotypeMammalian kidneyApical membraneK channelsROMK knockout miceKnockout modelsChannel activityChannel mutationsRenal functionLimb functionNull micePhysiological conditionsSalt wastingTransporter expressionPathophysiological conditions
2008
Female ROMK null mice manifest more severe Bartter II phenotype on renal function and higher PGE2 production
Yan Q, Yang X, Cantone A, Giebisch G, Hebert S, Wang T. Female ROMK null mice manifest more severe Bartter II phenotype on renal function and higher PGE2 production. AJP Regulatory Integrative And Comparative Physiology 2008, 295: r997-r1004. PMID: 18579648, PMCID: PMC2536865, DOI: 10.1152/ajpregu.00051.2007.Peer-Reviewed Original ResearchConceptsROMK null miceFemale null miceNull miceRenal functionSurvival rateExtent of hydronephrosisDegree of hydronephrosisSeverity of hydronephrosisHigher PGE2 productionHydronephrotic miceLower GFRSyndrome pathophysiologyNg/24 hAcid-base parametersFemale micePGE2 productionHydronephrosisHigher survival rateMiceExcretionSignificant differencesUrinaryMalesSexPossible mechanism
2004
NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice
Bailey MA, Giebisch G, Abbiati T, Aronson PS, Gawenis LR, Shull GE, Wang T. NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice. The Journal Of Physiology 2004, 561: 765-775. PMID: 15604231, PMCID: PMC1665379, DOI: 10.1113/jphysiol.2004.074716.Peer-Reviewed Original ResearchConceptsBicarbonate excretionDistal tubulesBicarbonate reabsorptionNHE3 null miceBicarbonate loadEarly distal tubule fluidNull miceNephron segmentsVivo micropunctureDistal tubule fluidProximal tubule bicarbonate reabsorptionRenal clearance experimentsUrinary bicarbonate excretionGlomerular filtration rateDistal nephron segmentsUrinary bicarbonate lossRat distal tubulesDistal bicarbonate reabsorptionExcretion of bicarbonateMetabolic acidosisFiltration rateVivo microperfusionClearance experimentsReabsorption capacityMicroperfusion experiments
2003
ROMK is required for expression of the 70-pS K channel in the thick ascending limb
Lu M, Wang T, Yan Q, Wang W, Giebisch G, Hebert SC. ROMK is required for expression of the 70-pS K channel in the thick ascending limb. American Journal Of Physiology. Renal Physiology 2003, 286: f490-f495. PMID: 14600033, DOI: 10.1152/ajprenal.00305.2003.Peer-Reviewed Original ResearchConceptsThick ascending limbBartter's syndromeK dietK channelsAscending limbChannel activityApical K channelsFunctional expressionHypokalemic alkalosisTAL cellsNull miceSK activityHeterozygous miceHeterogeneous disorderMicePotassium recyclingROMKFunction mutationsSyndromeCritical subunitApical conductanceSalt absorptionLimbDietExpressionInhibition of heme oxygenase decreases sodium and fluid absorption in the loop of Henle
Wang T, Sterling H, Shao W, Yan Q, Bailey M, Giebisch G, Wang W. Inhibition of heme oxygenase decreases sodium and fluid absorption in the loop of Henle. American Journal Of Physiology. Renal Physiology 2003, 285: f484-f490. PMID: 12890663, DOI: 10.1152/ajprenal.00135.2003.Peer-Reviewed Original ResearchConceptsLoop of HenleHigh K+K+ channelsApical 70-pS K+ channelRegulation of Na+ transportNa+ transportFluid absorptionApical K+ channelsIn vivo microperfusionInhibition of HORenal sodium excretionGlomerular filtration rateHO-2 expressionInhibitor of HORenal clearance studiesHK ratsHO-2K+ recyclingNa+ absorptionUrinary Na+Sodium excretionChromium mesoporphyrinRat chowWestern blot analysisFiltration rate
2000
Defective fluid and HCO3 − absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice
Wang T, Inglis F, Kalb R. Defective fluid and HCO3 − absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice. American Journal Of Physiology. Renal Physiology 2000, 279: f518-f524. PMID: 10966931, DOI: 10.1152/ajprenal.2000.279.3.f518.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsBicarbonatesBiological TransportBlood Gas AnalysisBody FluidsElectrolytesEnzyme InhibitorsInjections, IntravenousKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutNG-Nitroarginine Methyl EsterNitric Oxide SynthaseNitric Oxide Synthase Type IPotassiumSodiumConceptsNNOS knockout miceWild-type miceL-NAMEProximal tubulesMetabolic acidosisAbsorption of fluidDecreased absorption of fluidBlood pressureIncreased mean blood pressureResponse to L-NAMEWild-type control animalsFluid absorptionAdministration of L-NAMEIn situ microperfusionProximal tubule transportNeuronal nitric oxide synthaseLuminal perfusion solutionMean blood pressureRenal clearance techniquesNitric oxide synthaseRates of HCO(3)(-Urinary Na(+HCO3- absorptionRegulation of acid-base balanceTubule transport
1998
A tyrosine-based signal regulates H-K-ATPase-mediated potassium reabsorption in the kidney
Wang T, Courtois-Coutry N, Giebisch G, Caplan M. A tyrosine-based signal regulates H-K-ATPase-mediated potassium reabsorption in the kidney. American Journal Of Physiology 1998, 275: f818-f826. PMID: 9815140, DOI: 10.1152/ajprenal.1998.275.5.f818.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateTransgenic miceGastric acid outputPlasma K concentrationK pumpK-ATPaseRenal collecting tubulesK clearanceBlood pressurePotassium reabsorptionAcid outputUrine volumeK excretionFiltration rateGastric acidK reabsorptionPump functionCollecting tubuleMicePlasma NaTyrosine-based sequenceTyrosine-based signalsKidneyExcretionCytoplasmic tail
1996
Effects of angiotensin II on electrolyte transport in the early and late distal tubule in rat kidney
Wang T, Giebisch G. Effects of angiotensin II on electrolyte transport in the early and late distal tubule in rat kidney. American Journal Of Physiology 1996, 271: f143-f149. PMID: 8760255, DOI: 10.1152/ajprenal.1996.271.1.f143.Peer-Reviewed Original ResearchConceptsEffects of Ang IIAng IILate distal tubuleDistal tubulesDecreased JvAngiotensin IIHigher dose of Ang IILow doses of angiotensin IILuminal application of amilorideAmiloride-sensitive Na+ transportDose of Ang IIRat kidneyHCO3- transportAddition of ANG IIDoses of angiotensin IIEffects of angiotensin IIApplication of amilorideStimulate Na+/H+ exchangeProximal tubules of rat kidneyDistal convoluted tubuleLuminal administrationNa+ channelsNa+/H+ exchangeDistal nephronIntravenous infusion
1995
Effects of Glyburide on Renal Tubule Transport and Potassium-Channel Activity
Wang T, Wang W, Klein-Robbenhaar G, Giebisch G. Effects of Glyburide on Renal Tubule Transport and Potassium-Channel Activity. Kidney & Blood Pressure Research 1995, 18: 169-182. PMID: 7481068, DOI: 10.1159/000173914.Peer-Reviewed Original ResearchConceptsK channelsApical membraneInhibition of K secretionApical K channelsRenal tubule transportCortical collecting tubuleK channel activityPatch-clamp techniquePotassium channel activityEffect of glyburideTubule transportK secretionRenal clearanceElectrolyte excretionPotassium channelsTransport of NaAscending limbGlyburideInhibit transportK recyclingKaliuresisHenleMicroperfusionTubulesExcretion