2010
Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells
Duan Y, Weinstein AM, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 21860-21865. PMID: 21106755, PMCID: PMC3003103, DOI: 10.1073/pnas.1015751107.Peer-Reviewed Original ResearchConceptsV-ATPaseFluid shear stressActin cytoskeletonNa/K-ATPaseIntact actin cytoskeletonApical plasma membraneK-ATPaseIntact microtubule networkShear stress-induced changesCellular functionsProximal tubule cellsApical NHE3Plasma membraneTransporter localizationMicrotubule networkIntracellular compartmentsWestern blot analysisCytochalasin DTubule cellsMPT cellsMicrotubule polymerizationTraffickingMouse proximal tubule cellsBlot analysisConfocal microscopyAltered renal proximal tubular endocytosis and histology in mice lacking myosin‐VI
Gotoh N, Yan Q, Du Z, Biemesderfer D, Kashgarian M, Mooseker MS, Wang T. Altered renal proximal tubular endocytosis and histology in mice lacking myosin‐VI. Cytoskeleton 2010, 67: 178-192. PMID: 20175219, PMCID: PMC3468331, DOI: 10.1002/cm.20435.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAnimalsApoptosis Regulatory ProteinsCell TransdifferentiationEndocytosisHorseradish PeroxidaseKidney Tubules, ProximalLow Density Lipoprotein Receptor-Related Protein-2MiceMice, Neurologic MutantsMicrofilament ProteinsMicrovilliMyosin Heavy ChainsVesicular Transport ProteinsConceptsProximal tubule cellsAlbumin excretionSv miceTubule cellsProximal tubule brush borderGlomerular flow rateProximal tubular dilationUrinary albumin excretionElevated blood pressureReduced body weightProximal tubular endocytosisRenal proximal tubule brush borderTubule brush borderProximal tubular uptakeBlood acid-base parametersMouse kidney proximal tubulesKidney proximal tubulesBlood pressureUrine volumeUrinary excretionAcid-base parametersRenal homogenatesTubular dilationPlasma concentrationsRenal clearance
2006
Flow-activated transport events along the nephron
Wang T. Flow-activated transport events along the nephron. Current Opinion In Nephrology & Hypertension 2006, 15: 530-536. PMID: 16914967, DOI: 10.1097/01.mnh.0000242180.46362.c4.Peer-Reviewed Original ResearchConceptsProximal tubulesCultured proximal tubule cellsGlomerular filtration rateProximal tubule cellsSystemic hormonal regulationMouse proximal tubulesFiltration rateH-ATPase activityHCO3- absorptionTubule cellsReceptor localizationENOS translocationNHE3 activityHormonal regulationBrush border microvilliTubulesNephronSecond messengerUnanswered questionsTransduction mechanisms
2003
Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice
Beck F, Neuhofer W, Dörge A, Giebisch G, Wang T. Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice. Pflügers Archiv - European Journal Of Physiology 2003, 446: 100-105. PMID: 12690468, DOI: 10.1007/s00424-002-1001-z.Peer-Reviewed Original ResearchConceptsIntracellular Na concentrationNa/K-ATPaseΑ1 subunitTubule cellsProximal convoluted tubule cellsDistal convoluted tubuleWild-type miceNa/K-ATPase activityPCT cellsConvoluted tubule cellsIntracellular Cl- concentrationK-ATPaseWestern blot analysisK-ATPase activityNa concentrationRenal cortexCell Na concentrationConvoluted tubulesNa/K-ATPase α1 subunitNa/HNHE3 deficiencyK-ATPase α1-subunitMiceFluid absorption
1995
Stimulation of chloride transport by cAMP in rat proximal tubules
Wang T, Segal AS, Giebisch G, Aronson PS. Stimulation of chloride transport by cAMP in rat proximal tubules. American Journal Of Physiology 1995, 268: f204-f210. PMID: 7864157, DOI: 10.1152/ajprenal.1995.268.2.f204.Peer-Reviewed Original ResearchConceptsRat proximal tubuleProximal tubulesDibutyryl cAMPCapillary perfusateLate proximal tubuleProximal tubule cellsCl- channel blockerCl- channelsTranscellular Cl absorptionAddition of forskolinLuminal DIDSSensitive Cl- channelBasolateral membraneLuminal perfusateTubule cellsCAMP productionFluid absorptionCl absorptionMM ClPerfusateJclRecent evidenceLuminal membraneTubulesEffect of cAMP