2019
Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle
Aung TN, Nourmohammadi S, Qu Z, Harata-Lee Y, Cui J, Shen HY, Yool AJ, Pukala T, Du H, Kortschak RD, Wei W, Adelson DL. Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle. Scientific Reports 2019, 9: 14200. PMID: 31578346, PMCID: PMC6775143, DOI: 10.1038/s41598-019-50271-4.Peer-Reviewed Original ResearchConceptsCompound Kushen InjectionExperimental biology approachesEffect of CKIPlant secondary metabolitesCytokine Signaling PathwaysCKI activityBiology approachReconstitution approachGene expressionCell cyclePhenotype dataSignaling pathwaysSecondary metabolitesBreast cancer cell linesCancer cell linesKushen InjectionSingle major compoundCell linesApoptosisLiquid chromatography fractionationCell viabilityHigh-performance liquid chromatography fractionationMajor compoundsComplex fashionCandidate mechanismEffect of Compound Kushen Injection, a Natural Compound Mixture, and Its Identified Chemical Components on Migration and Invasion of Colon, Brain, and Breast Cancer Cell Lines
Nourmohammadi S, Aung TN, Cui J, Pei JV, De Ieso ML, Harata-Lee Y, Qu Z, Adelson DL, Yool AJ. Effect of Compound Kushen Injection, a Natural Compound Mixture, and Its Identified Chemical Components on Migration and Invasion of Colon, Brain, and Breast Cancer Cell Lines. Frontiers In Oncology 2019, 9: 314. PMID: 31106149, PMCID: PMC6498862, DOI: 10.3389/fonc.2019.00314.Peer-Reviewed Original ResearchCompound Kushen InjectionCell linesFocal adhesion genesBreast cancer cell linesCancer cell linesCell imagingActin cytoskeletalHuman embryonic kidneyTranscriptomic analysisAdhesion genesMDA-MB-231 cellsCell cycleBreast cancerInvasion of colonKushen InjectionEmbryonic kidneyCell migrationExtracellular matrixTranswell invasionNatural compound mixturesCKI treatmentHEK-293Cancer migrationNon-cancerous controlsCancer metastasis
2016
Identification of candidate anti-cancer molecular mechanisms of compound kushen injection using functional genomics
Qu Z, Cui J, Harata-Lee Y, Aung TN, Feng Q, Raison JM, Kortschak RD, Adelson DL. Identification of candidate anti-cancer molecular mechanisms of compound kushen injection using functional genomics. Oncotarget 2016, 7: 66003-66019. PMID: 27602759, PMCID: PMC5323210, DOI: 10.18632/oncotarget.11788.Peer-Reviewed Original ResearchConceptsCompound Kushen InjectionAnti-cancer molecular mechanismMolecular mechanismsHigh-throughput Illumina RNA-Seq technologyIllumina RNA-Seq technologyMCF-7 cellsKushen InjectionRNA-seq technologyEffect of CKITranscriptome analysis methodsLong non-coding RNAsPlant secondary metabolitesNon-coding RNAsDifferential expression analysisCell proliferationGene differential expression analysisMCF-7 human breast cancer cell lineDe novo identificationHuman breast cancer cell linesMCF-7 cell proliferationBreast cancer cell linesDose-dependent fashionAnnexin V/propidium iodideTraditional Chinese medicine preparationP53-independent mechanism