2022
Identification of novel conserved Ixodes vaccine candidates; a promising role for non-secreted salivary gland proteins
Trentelman J, de Vogel F, Colstrup E, Sima R, Coumou J, Koetsveld J, Klouwens M, Nayak A, Ersoz J, Barriales D, Tomás-Cortázar J, Narasimhan S, Hajdusek O, Anguita J, Hovius J. Identification of novel conserved Ixodes vaccine candidates; a promising role for non-secreted salivary gland proteins. Vaccine 2022, 40: 7593-7603. PMID: 36357287, DOI: 10.1016/j.vaccine.2022.10.032.Peer-Reviewed Original ResearchConceptsTick salivary gland proteinsAnti-tick vaccinesTick immunityVaccine candidatesSalivary gland proteinsLyme borreliosisNymphal I. ricinusExperimental Lyme borreliosisImmune animalsVaccination studiesTick feedingI. ricinusTick attachmentNymphal ticksPathogen transmissionGuinea pigsIxodes scapularisI. scapularisGland proteinsVaccineCausative agentImmunityMain vectorIdentification of novelPromising role
2021
Tick immunity using mRNA, DNA and protein-based Salp14 delivery strategies
Matias J, Kurokawa C, Sajid A, Narasimhan S, Arora G, Diktas H, Lynn GE, DePonte K, Pardi N, Valenzuela JG, Weissman D, Fikrig E. Tick immunity using mRNA, DNA and protein-based Salp14 delivery strategies. Vaccine 2021, 39: 7661-7668. PMID: 34862075, PMCID: PMC8671329, DOI: 10.1016/j.vaccine.2021.11.003.Peer-Reviewed Original ResearchConceptsTick bite siteTick immunityAntigen deliveryBite siteGuinea pigsDevelopment of vaccinesIxodes scapularis ticksProtein immunizationAntibody responseTick biteVaccine platformLipid nanoparticlesMRNA lipid nanoparticlesMRNA-LNPModel antigenTick salivaEarly hallmarkImmunityTick-borne diseasesScapularis ticksTick challengeErythemaSalivary componentsFactor XaDelivery strategiesProbing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
Trentelman JJA, Tomás-Cortázar J, Knorr S, Barriales D, Hajdusek O, Sima R, Ersoz JI, Narasimhan S, Fikrig E, Nijhof AM, Anguita J, Hovius JW. Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine. Scientific Reports 2021, 11: 15745. PMID: 34344917, PMCID: PMC8333314, DOI: 10.1038/s41598-021-92538-9.Peer-Reviewed Original ResearchConceptsTick salivary gland proteinsLyme borreliosisVaccination studiesTick biteTick-borne encephalitis virusB. burgdorferi transmissionMultiple tick bitesYeast surface display libraryHuman infectious diseasesImmunization of rabbitsVaccination platformAnti-tick effectsAnti-tick vaccinesEncephalitis virusImmunodominant antigensInfectious diseasesNon-natural hostsTick immunityTick feedingImmunityBorreliosisBiteVaccineAntigenHuman serum
2020
Fractionation of tick saliva reveals proteins associated with the development of acquired resistance to Ixodes scapularis
Černý J, Lynn G, DePonte K, Ledizet M, Narasimhan S, Fikrig E. Fractionation of tick saliva reveals proteins associated with the development of acquired resistance to Ixodes scapularis. Vaccine 2020, 38: 8121-8129. PMID: 33168347, DOI: 10.1016/j.vaccine.2020.10.087.Peer-Reviewed Original ResearchConceptsTick-borne pathogensTick immunityTick salivaTick vaccinesMultiple tick-borne pathogensIxodes scapularisGuinea pig modelTick salivary antigensTick rejectionTick Ixodes scapularisPig modelTick feedingNortheast USASalivary antigensGlobal medical problemDevelopment of vaccinesTicksMain vectorPathogensSaliva fractionsScapularisMedical problemsImmunityVaccineSaliva