2016
Long-lived antigen-induced IgM plasma cells demonstrate somatic mutations and contribute to long-term protection
Bohannon C, Powers R, Satyabhama L, Cui A, Tipton C, Michaeli M, Skountzou I, Mittler RS, Kleinstein SH, Mehr R, Lee FE, Sanz I, Jacob J. Long-lived antigen-induced IgM plasma cells demonstrate somatic mutations and contribute to long-term protection. Nature Communications 2016, 7: 11826. PMID: 27270306, PMCID: PMC4899631, DOI: 10.1038/ncomms11826.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAmino Acid MotifsAnimalsAntigensComplementarity Determining RegionsCytidine DeaminaseGerminal CenterImmunityImmunoglobulin Heavy ChainsImmunoglobulin MMice, Inbred C57BLMutationNeutralization TestsOrthomyxoviridaeOrthomyxoviridae InfectionsPlasma CellsSomatic Hypermutation, ImmunoglobulinSpleenConceptsIgM plasma cellsIgG plasma cellsPlasma cellsGerminal centersBone marrowLethal virus challengeProtective host immunitySomatic mutationsActivation-induced cytidine deaminaseHumoral immunityProtective antibodiesVirus challengeLong-term protectionHost immunityB cellsAffinity maturationMarrowLifelong sourceImmunityAntibodiesCellsCytidine deaminaseMutationsReplacement mutationsSpleen
2014
DNA Demethylation By Activation-Induced Cytidine Deaminase in B Cell Lymphoma
Xi Y, Shivarov V, Yaari G, Kleinstein S, Strout M. DNA Demethylation By Activation-Induced Cytidine Deaminase in B Cell Lymphoma. Blood 2014, 124: 3549. DOI: 10.1182/blood.v124.21.3549.3549.Peer-Reviewed Original ResearchActivation-induced cytidine deaminaseDNA demethylationGene expressionDNA methylationMethylation sitesBisulfite sequencingBL2 cellsHumanHT-12 v4 Expression BeadChipPrimary transcriptional start sitesC-terminal deletion mutantsCpG sitesGenome-wide methylation profilingActive DNA demethylationMethylation statusRNA polymerase IISomatic cell differentiationCytidine deaminaseSingle-nucleotide resolutionTranscriptional start siteSubset of genesDNA repair pathwaysList of genesTarget gene expressionEarly cell developmentV4 Expression BeadChip