2011
Reduced CGP12177 binding to cardiac β-adrenoceptors in hyperglycemic high-fat-diet-fed, streptozotocin-induced diabetic rats
Thackeray JT, Parsa-Nezhad M, Kenk M, Thorn SL, Kolajova M, Beanlands RS, DaSilva JN. Reduced CGP12177 binding to cardiac β-adrenoceptors in hyperglycemic high-fat-diet-fed, streptozotocin-induced diabetic rats. Nuclear Medicine And Biology 2011, 38: 1059-1066. PMID: 21831645, DOI: 10.1016/j.nucmedbio.2011.04.002.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic beta-AntagonistsAnimalsBiomarkersBlotting, WesternDiabetes Mellitus, ExperimentalDiet, High-FatGene Expression RegulationHyperglycemiaMaleMyocardiumPositron-Emission TomographyPropanolaminesProtein BindingRatsRats, Sprague-DawleyReceptors, Adrenergic, beta-1Receptors, Adrenergic, beta-2Substrate SpecificityConceptsAbnormal sympathetic nervous systemΒ-AR expressionSympathetic nervous systemDiabetic rat heartsSingle intraperitoneal injectionVehicle-treated controlsInfusion of isoproterenolCardiac β-adrenoceptorsΒ-AR subtypesΒ-AR antagonistsSpecific bindingHalf of ratsNormal Sprague-DawleyΒ-AR stimulationPositron emission tomographyΒ-adrenoceptor signalingCardiac bindingAR expressionCardiac dysfunctionDiabetic ratsInsulin resistanceSustained hyperglycemiaIntraperitoneal injectionΒ-adrenoceptorsDiet feeding
2006
In vivo selective binding of (R)-[11C]rolipram to phosphodiesterase-4 provides the basis for studying intracellular cAMP signaling in the myocardium and other peripheral tissues
Kenk M, Greene M, Thackeray J, deKemp RA, Lortie M, Thorn S, Beanlands RS, DaSilva JN. In vivo selective binding of (R)-[11C]rolipram to phosphodiesterase-4 provides the basis for studying intracellular cAMP signaling in the myocardium and other peripheral tissues. Nuclear Medicine And Biology 2006, 34: 71-77. PMID: 17210463, DOI: 10.1016/j.nucmedbio.2006.10.002.Peer-Reviewed Original ResearchMeSH Keywords3',5'-Cyclic-AMP PhosphodiesterasesAnimalsBrainCarbon RadioisotopesCyclic AMPCyclic Nucleotide Phosphodiesterases, Type 1Cyclic Nucleotide Phosphodiesterases, Type 4HeartMaleMetabolic Clearance RateMyocardiumOrgan SpecificityPhosphodiesterase InhibitorsProtein BindingRadionuclide ImagingRadiopharmaceuticalsRatsRats, Sprague-DawleyRolipramSensitivity and SpecificityTissue DistributionConceptsPhosphodiesterase 4BAY 60Ro 20Male Sprague-Dawley ratsIntracellular cAMPSprague-Dawley ratsNeurohormonal modulationPeripheral tissuesAutoradiography studiesAdipose tissuePDE4 levelsTracer uptakeVivo findingsCAMP-mediated signalingBiodistribution studiesPDE4 activityRolipramSkeletal muscleCAMP levelsTracer retentionCardiac regionCilostazolMyocardiumZaprinastTissue