2021
TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations
Cheng Y, Liu M, Hu M, Wang S. TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations. Genome Biology 2021, 22: 309. PMID: 34749781, PMCID: PMC8574027, DOI: 10.1186/s13059-021-02523-8.Peer-Reviewed Original ResearchConceptsInactive X chromosomeActive X chromosomeMajor epigenetic componentsSingle-cell domainsX chromosomeEpigenetic componentsThree-dimensional genome architectureGlobal epigenetic landscapeFemale human cellsLoop extrusion mechanismSame genomic regionGenome architectureChromatin domainsTAD structureChromatin compactionEpigenetic landscapeTAD boundariesChromatin foldingGenomic regionsChromosome copiesGenomic techniquesEpigenetic perturbationsEpigenetic interactionsDistinct cell linesChromosomesChromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue
Liu M, Yang B, Hu M, Radda JSD, Chen Y, Jin S, Cheng Y, Wang S. Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue. Nature Protocols 2021, 16: 2667-2697. PMID: 33903756, PMCID: PMC9007104, DOI: 10.1038/s41596-021-00518-0.Peer-Reviewed Original ResearchConceptsSame single cellNucleome architecturesGene expressionMammalian tissuesChromatin foldingNuclear laminaSingle cellsNumerous RNA speciesDifferent biological processesSingle mammalian cellsDifferent cell typesMultiplexed imagingGenomic organizationGenomic architectureChromatin loopsGenomic regionsRNA speciesIndividual chromosomesMammalian cellsGenomic techniquesBiological processesDetailed protocolCopy numberCell typesNormal development
2020
Chromatin Tracing: Imaging 3D Genome and Nucleome
Hu M, Wang S. Chromatin Tracing: Imaging 3D Genome and Nucleome. Trends In Cell Biology 2020, 31: 5-8. PMID: 33191055, PMCID: PMC8094612, DOI: 10.1016/j.tcb.2020.10.006.Peer-Reviewed Original Research