Immunoprecipitation of cell lysates with RAP-5 does not specifically detect ras oncogene product p21
Gutheil J, Mane S, Kapil V, Needleman S. Immunoprecipitation of cell lysates with RAP-5 does not specifically detect ras oncogene product p21. Human Pathology 1989, 20: 1176-1180. PMID: 2687155, DOI: 10.1016/s0046-8177(89)80008-5.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalBreast NeoplasmsCell LinePrecipitin TestsProto-Oncogene ProteinsProto-Oncogene Proteins p21(ras)Purification and characterization of human lysosomal membrane glycoproteins
Mane S, Marzella L, Bainton D, Holt V, Cha Y, Hildreth J, August J. Purification and characterization of human lysosomal membrane glycoproteins. Archives Of Biochemistry And Biophysics 1989, 268: 360-378. PMID: 2912382, DOI: 10.1016/0003-9861(89)90597-3.Peer-Reviewed Original ResearchConceptsLysosomal membrane glycoproteinsCell surface expressionHL-60 cellsPlasma membraneU937 myelomonocytic leukemia cellsMembrane glycoproteinsHuman lysosomal membrane glycoproteinsU937 cellsAddition of AsnDetergent-extractable proteinTissue culture cellsNH2-terminal sequence analysisHLAMP-2Mouse cellsMembrane flowSequence analysisMyelomonocytic leukemia cellsAntibody affinity chromatographyCell typesCulture cellsPeroxidase-positive granulesProteinHLAMP-1Lysosomal structuresAffinity chromatography