2017
Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells
Whirledge S, Kisanga EP, Taylor RN, Cidlowski JA. Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells. Endocrinology 2017, 158: 4076-4092. PMID: 28938408, PMCID: PMC5695839, DOI: 10.1210/en.2017-00361.Peer-Reviewed Original ResearchConceptsPioneer factor FOXA1Polymerase 2Molecular mechanismsRecruitment of GRThousands of genesExpression of lefty1GR-dependent inductionGene expression analysisHuman endometrial cellsGlucocorticoid receptorGlucocorticoid-mediated inductionEndometrial cellsRegulatory regionsGlucocorticoid receptor signalingMaster regulatorSpecific genesExpression analysisPrimary human endometrial cellsGene expressionHuman uterine cellsGlucocorticoid-dependent inductionIntracellular signalingEmbryo implantationFOXA2Receptor recruitmentGenerating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor
Whirledge SD, Jewell CM, Barber LM, Xu X, Katen KS, Garantziotis S, Cidlowski JA. Generating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor. Laboratory Investigation 2017, 97: 1282-1295. PMID: 28759007, PMCID: PMC5759773, DOI: 10.1038/labinvest.2017.76.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdultAmino Acid SubstitutionBlack PeopleCell Line, TumorCells, CulturedCohort StudiesFemaleGene Expression RegulationGenetic Association StudiesGlucocorticoidsHispanic or LatinoHumansMacrophagesMaleNorth CarolinaPolymorphism, Single NucleotideProspective StudiesReceptors, GlucocorticoidRecombinant ProteinsRegistriesSignal TransductionWhite PeopleConceptsGene expression profilesGene expressionExpression profilesGlucocorticoid sensitivityT polymorphismWide gene expressionPrimary macrophagesInherent transcriptional activityHigher serum cortisol levelsLow-dose dexamethasoneProspective cohort studyProtein expressionSelf-reported allergiesDominant negative regulatorSerum cortisol levelsReceptor protein expressionSelf-reported diseasesGlucocorticoid receptor geneHuman glucocorticoid receptor geneMinor allele frequencyNon-Hispanic participantsAlternative splicingMRNA stabilityReceptor stabilityTranscriptional activityGlucocorticoids and Reproduction: Traffic Control on the Road to Reproduction
Whirledge S, Cidlowski JA. Glucocorticoids and Reproduction: Traffic Control on the Road to Reproduction. Trends In Endocrinology And Metabolism 2017, 28: 399-415. PMID: 28274682, PMCID: PMC5438761, DOI: 10.1016/j.tem.2017.02.005.Peer-Reviewed Original ResearchConceptsDiverse cellular functionsStress-induced levelsCell-specific mannerCellular functionsGlucocorticoid receptorCellular processingGR geneReproductive functionNormal physiologyActivity of glucocorticoidsCompelling new evidenceReproductionReproductive dysfunctionGlucocorticoid responsivenessGlucocorticoidsSteroid hormonesGenesProteinDiversityPhysiologyRegulationFunctionReceptorsNew evidenceMaintenance
2015
Uterine glucocorticoid receptors are critical for fertility in mice through control of embryo implantation and decidualization
Whirledge SD, Oakley RH, Myers PH, Lydon JP, DeMayo F, Cidlowski JA. Uterine glucocorticoid receptors are critical for fertility in mice through control of embryo implantation and decidualization. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 15166-15171. PMID: 26598666, PMCID: PMC4679013, DOI: 10.1073/pnas.1508056112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDeciduaEmbryo ImplantationFemaleFertilityMiceMice, KnockoutReceptors, GlucocorticoidUterusConceptsGlucocorticoid receptorPresence of GRExaggerated inflammatory responseSex steroid receptorsImmune cell recruitmentStromal cell decidualizationFemale reproductive tractAdrenal axisKO miceInflammatory responseUterine physiologyUterine biologyEndocrine organMouse uterusBlastocyst implantationGlucocorticoid signalingMouse modelCell recruitmentEmbryo implantationImmunomodulatory functionsKnockout miceGR signalingSteroid receptorsReproductive tractDecidualization
2014
Genistein Disrupts Glucocorticoid Receptor Signaling in Human Uterine Endometrial Ishikawa Cells
Whirledge S, Senbanjo LT, Cidlowski JA. Genistein Disrupts Glucocorticoid Receptor Signaling in Human Uterine Endometrial Ishikawa Cells. Environmental Health Perspectives 2014, 123: 80-87. PMID: 25136773, PMCID: PMC4286279, DOI: 10.1289/ehp.1408437.Peer-Reviewed Original ResearchConceptsTarget genesWhole-genome microarray analysisIshikawa cellsGlucocorticoid receptorGenome microarray analysisCommon gene targetsGR target genesTranscriptional responseNumerous genesDisruption of GRTranscriptional antagonismGlucocorticoid receptor signalingEnvironmental estrogen exposureRegulation of stressGene targetsSelect genesGene expressionCoadministration of genisteinEndometrial Ishikawa cellsMicroarray analysisReal-time reverse transcription-polymerase chain reactionFemale reproductive tractReverse transcription-polymerase chain reactionGenesQuantitative real-time reverse transcription-polymerase chain reaction
2012
Glucocorticoids Regulate Gene Expression and Repress Cellular Proliferation in Human Uterine Leiomyoma Cells
Whirledge S, Dixon D, Cidlowski JA. Glucocorticoids Regulate Gene Expression and Repress Cellular Proliferation in Human Uterine Leiomyoma Cells. Discover Oncology 2012, 3: 79-92. PMID: 22311344, PMCID: PMC4772901, DOI: 10.1007/s12672-012-0103-0.Peer-Reviewed Original ResearchConceptsUterine leiomyoma cellsHuman uterine leiomyoma cellsLeiomyoma cellsGlucocorticoid receptorUterine leiomyomaUterine leiomyoma treatmentAbsence of estrogenPotential roleSmooth muscle cellsSynthetic glucocorticoid dexamethasoneIngenuity Pathway AnalysisLeiomyoma treatmentSignificant glucocorticoidE2 effectsEstrogen treatmentCell growthSex hormonesGenome-wide microarray studiesGlucocorticoid dexamethasoneGlucocorticoidsS-phase cellsNormal myometriumFlow cytometryMuscle cellsLeiomyoma