2017
Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells
Whirledge S, Kisanga EP, Taylor RN, Cidlowski JA. Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells. Endocrinology 2017, 158: 4076-4092. PMID: 28938408, PMCID: PMC5695839, DOI: 10.1210/en.2017-00361.Peer-Reviewed Original ResearchConceptsPioneer factor FOXA1Polymerase 2Molecular mechanismsRecruitment of GRThousands of genesExpression of lefty1GR-dependent inductionGene expression analysisHuman endometrial cellsGlucocorticoid receptorGlucocorticoid-mediated inductionEndometrial cellsRegulatory regionsGlucocorticoid receptor signalingMaster regulatorSpecific genesExpression analysisPrimary human endometrial cellsGene expressionHuman uterine cellsGlucocorticoid-dependent inductionIntracellular signalingEmbryo implantationFOXA2Receptor recruitmentGenerating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor
Whirledge SD, Jewell CM, Barber LM, Xu X, Katen KS, Garantziotis S, Cidlowski JA. Generating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor. Laboratory Investigation 2017, 97: 1282-1295. PMID: 28759007, PMCID: PMC5759773, DOI: 10.1038/labinvest.2017.76.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdultAmino Acid SubstitutionBlack PeopleCell Line, TumorCells, CulturedCohort StudiesFemaleGene Expression RegulationGenetic Association StudiesGlucocorticoidsHispanic or LatinoHumansMacrophagesMaleNorth CarolinaPolymorphism, Single NucleotideProspective StudiesReceptors, GlucocorticoidRecombinant ProteinsRegistriesSignal TransductionWhite PeopleConceptsGene expression profilesGene expressionExpression profilesGlucocorticoid sensitivityT polymorphismWide gene expressionPrimary macrophagesInherent transcriptional activityHigher serum cortisol levelsLow-dose dexamethasoneProspective cohort studyProtein expressionSelf-reported allergiesDominant negative regulatorSerum cortisol levelsReceptor protein expressionSelf-reported diseasesGlucocorticoid receptor geneHuman glucocorticoid receptor geneMinor allele frequencyNon-Hispanic participantsAlternative splicingMRNA stabilityReceptor stabilityTranscriptional activity
2014
Genistein Disrupts Glucocorticoid Receptor Signaling in Human Uterine Endometrial Ishikawa Cells
Whirledge S, Senbanjo LT, Cidlowski JA. Genistein Disrupts Glucocorticoid Receptor Signaling in Human Uterine Endometrial Ishikawa Cells. Environmental Health Perspectives 2014, 123: 80-87. PMID: 25136773, PMCID: PMC4286279, DOI: 10.1289/ehp.1408437.Peer-Reviewed Original ResearchConceptsTarget genesWhole-genome microarray analysisIshikawa cellsGlucocorticoid receptorGenome microarray analysisCommon gene targetsGR target genesTranscriptional responseNumerous genesDisruption of GRTranscriptional antagonismGlucocorticoid receptor signalingEnvironmental estrogen exposureRegulation of stressGene targetsSelect genesGene expressionCoadministration of genisteinEndometrial Ishikawa cellsMicroarray analysisReal-time reverse transcription-polymerase chain reactionFemale reproductive tractReverse transcription-polymerase chain reactionGenesQuantitative real-time reverse transcription-polymerase chain reaction
2012
Estradiol Antagonism of Glucocorticoid-Induced GILZ Expression in Human Uterine Epithelial Cells and Murine Uterus
Whirledge S, Cidlowski JA. Estradiol Antagonism of Glucocorticoid-Induced GILZ Expression in Human Uterine Epithelial Cells and Murine Uterus. Endocrinology 2012, 154: 499-510. PMID: 23183181, PMCID: PMC3529382, DOI: 10.1210/en.2012-1748.Peer-Reviewed Original ResearchConceptsGILZ gene expressionGILZ expressionEstrogen receptorGlucocorticoid receptorInterplay of estrogensSex hormone signalingEpithelial cellsER antagonist ICIImmune modulating functionsHuman uterine epithelial cellsCombination of dexamethasoneUterine epithelial cellsDexamethasone decreasesImmune-related functionsGlucocorticoid response elementMurine uterusGene expressionMouse uterusGlucocorticoid signalingEmbryo implantationUterine endometriumGILZ promoterGlucocorticoidsHormone treatmentReceptors