Optimization of techniques for multiple platform testing in small, precious samples such as human chorionic villus sampling
Pisarska MD, Akhlaghpour M, Lee B, Barlow GM, Xu N, Wang ET, Mackey AJ, Farber CR, Rich SS, Rotter JI, Chen Y, Goodarzi MO, Guller S, Williams J. Optimization of techniques for multiple platform testing in small, precious samples such as human chorionic villus sampling. Prenatal Diagnosis 2016, 36: 1061-1070. PMID: 27718505, PMCID: PMC5142835, DOI: 10.1002/pd.4936.Peer-Reviewed Original ResearchConceptsGenome-wide methylation profilingRNA-seq librariesSystems biology approachGene expression studiesBiology approachEpigenetic modificationsLow DNA yieldRNA sequencingMethylation profilingExpression studiesGene expressionPurification of DNAQuality DNAGlobal changeHighest RNA yieldDNA yieldRNA yieldRNADNAIsolation kitRNAlater samplesRNAlaterGenesChorionic villiMultiple testing