2019
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines.
Kisanga EP, Tang Z, Guller S, Whirledge S. In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines. Journal Of Visualized Experiments 2019 PMID: 30907868, DOI: 10.3791/58942.Peer-Reviewed Original ResearchConceptsCell movementCell migrationHuman first trimester trophoblast cell lineFirst trimester trophoblast cell lineIntrauterine growth restrictionCell linesTrophoblast cell linePregnancy disordersEarly pregnancyGrowth restrictionMaternal vasculatureTrophoblast migrationMigratory capacityPlacental developmentPotential mechanismsCellular changesVitro assaysInvasionTrophoblastProliferationThorough examinationMigrationAssaysIndividual factorsPregnancy
2018
Excess glucose induce trophoblast inflammation and limit cell migration through HMGB1 activation of Toll‐Like receptor 4
Heim KR, Mulla MJ, Potter JA, Han CS, Guller S, Abrahams VM. Excess glucose induce trophoblast inflammation and limit cell migration through HMGB1 activation of Toll‐Like receptor 4. American Journal Of Reproductive Immunology 2018, 80: e13044. PMID: 30175447, DOI: 10.1111/aji.13044.Peer-Reviewed Original ResearchConceptsToll-like receptor 4High mobility group box 1Damage-associated molecular patternsHMGB1 activationIL-1βIL-8Trophoblast responsesReceptor 4Human first trimester trophoblast cell lineFirst trimester trophoblast cell lineInflammatory IL-8Risk of preeclampsiaGroup box 1Uric acidIL-1β responseExcess glucoseTrophoblast cell lineCell migrationTrophoblast inflammationPregnancy outcomesTLR4 antagonistLPS-RSIL-6SFlt-1HMGB1 inhibitor
2009
The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71
Straszewski-Chavez SL, Abrahams VM, Alvero AB, Aldo PB, Ma Y, Guller S, Romero R, Mor G. The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71. Placenta 2009, 30: 939-948. PMID: 19766308, PMCID: PMC2784169, DOI: 10.1016/j.placenta.2009.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticApoptosisCell LineChorionic GonadotropinCytokinesFemaleFibronectinsHistocompatibility Antigens Class IHLA AntigensHLA-G AntigensHumansIntercellular Signaling Peptides and ProteinsKeratin-7Leukocyte Common AntigensPregnancyPregnancy Trimester, FirstTelomeraseThy-1 AntigensTrophoblastsVimentinConceptsFirst trimester trophoblast cell lineSwan 71 cellsTrophoblast cell lineFirst trimester trophoblast cellsFibroblast-specific antigenHuman chorionic gonadotrophinPrimary trophoblast cellsTrophoblast cellsHuman telomerase reverse transcriptaseFetal fibronectinCell linesCD90/ThySwan 71Growth factor profileTNF-alpha-induced apoptosisChorionic gonadotrophinCytokeratin 7Specific antigenTelomerase reverse transcriptaseNormal placentaFactor profileKaryotypic abnormalitiesPatient samplesPrimary human cellsAdequate cell numbers