2022
Regulation of EGF-stimulated activation of the PI-3K/AKT pathway by exocyst-mediated exocytosis
An S, Anneken A, Xi Z, Choi C, Schlessinger J, Toomre D. Regulation of EGF-stimulated activation of the PI-3K/AKT pathway by exocyst-mediated exocytosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2208947119. PMID: 36417441, PMCID: PMC9860279, DOI: 10.1073/pnas.2208947119.Peer-Reviewed Original ResearchConceptsPI-3K/Akt pathwayAkt pathwayAkt activationDocking protein Gab1EGF-stimulated activationEpithelial cellsLive-cell imagingPhosphoinositide-3 kinaseCell survival pathwaysExocyst complexExocyst functionSmall molecule inhibitorsVesicle tethersExocytic fusionProtein Gab1EGF stimulationExocystSurvival pathwaysExocytosisInhibitors resultsPathwayImportant pathwayEGFR inhibitionMinute time scaleVesicles
2021
An active tethering mechanism controls the fate of vesicles
An SJ, Rivera-Molina F, Anneken A, Xi Z, McNellis B, Polejaev VI, Toomre D. An active tethering mechanism controls the fate of vesicles. Nature Communications 2021, 12: 5434. PMID: 34521845, PMCID: PMC8440521, DOI: 10.1038/s41467-021-25465-y.Peer-Reviewed Original ResearchConceptsArtificial tetherFull fusionOptogenetic controlExocyst complexExocyst functionVesicle tethersMembrane mergerTethering mechanismTarget membraneIntracellular fusionPlasma membraneMode of fusionVesicle fusionPhysiological relevanceLamellipodial expansionVesiclesTetheringExocystMembraneFusionFurther showTetherFusion modeFateComplexes