2017
Comparative Membrane Proteomics Reveals a Nonannotated E. coli Heat Shock Protein
Yuan P, D’Lima N, Slavoff SA. Comparative Membrane Proteomics Reveals a Nonannotated E. coli Heat Shock Protein. Biochemistry 2017, 57: 56-60. PMID: 29039649, PMCID: PMC5761644, DOI: 10.1021/acs.biochem.7b00864.Peer-Reviewed Original ResearchMeSH KeywordsChromatography, High Pressure LiquidEscherichia coliEscherichia coli K12Escherichia coli ProteinsGene Expression Regulation, BacterialGreen Fluorescent ProteinsHeat-Shock ProteinsHeat-Shock Proteins, SmallHeat-Shock ResponseMembrane ProteinsModels, MolecularMolecular Sequence AnnotationOpen Reading FramesPhosphogluconate DehydrogenaseProtein Conformation, alpha-HelicalProtein Interaction Domains and MotifsProtein TransportProteogenomicsProteomicsRecombinant Fusion ProteinsTandem Mass SpectrometryConceptsHeat shock proteinsShock proteinsMembrane proteomicsE. coli heat shock proteinsComparative membrane proteomicsSmall open reading framesMembrane protein enrichmentQuantitative membrane proteomicsQuantitative proteomics protocolBacterial stress responseQuantitative mass spectrometryOpen reading frameDiscovery of thousandsEscherichia coli K12Transmembrane helicesProteomics protocolMembrane proteinsEvolutionary spaceReading frameSmall proteinsStress responseProteomicsColi K12Amino acidsProtein enrichment
2008
Expanding the Substrate Tolerance of Biotin Ligase through Exploration of Enzymes from Diverse Species
Slavoff SA, Chen I, Choi YA, Ting AY. Expanding the Substrate Tolerance of Biotin Ligase through Exploration of Enzymes from Diverse Species. Journal Of The American Chemical Society 2008, 130: 1160-1162. PMID: 18171066, PMCID: PMC3501195, DOI: 10.1021/ja076655i.Peer-Reviewed Original ResearchConceptsLigation reactionProtein labeling applicationsDifferential substrate specificitySite-specific conjugationBiotin acceptor domainPhosphine probesStaudinger ligationDiverse speciesBiotin ligaseChemical probesDifferent organismsMass spectrometry assayPyrococcus horikoshiiSubstrate specificityCell biologyUnnatural analoguesLabeling applicationsAcceptor domainsSubstrate toleranceLigasesDifferent speciesSpectrometry assayReactionSpeciesBiotin
2006
Isolation and characterisation of selected germander diterpenoids from authenticated Teucrium chamaedrys and T. canadense by HPLC, HPLC‐MS and NMR
Sundaresan PR, Slavoff SA, Grundel E, White KD, Mazzola E, Koblenz D, Rader JI. Isolation and characterisation of selected germander diterpenoids from authenticated Teucrium chamaedrys and T. canadense by HPLC, HPLC‐MS and NMR. Phytochemical Analysis 2006, 17: 243-250. PMID: 16910040, DOI: 10.1002/pca.912.Peer-Reviewed Original ResearchChromatography, High Pressure LiquidDiterpenesNuclear Magnetic Resonance, BiomolecularSpectrometry, Mass, Electrospray IonizationTeucrium