2021
Distinct Roles of Type I and Type III Interferons during a Native Murine β Coronavirus Lung Infection
Sharma L, Peng X, Qing H, Hilliard BK, Kim J, Swaminathan A, Tian J, Israni-Winger K, Zhang C, Habet V, Wang L, Gupta G, Tian X, Ma Y, Shin HJ, Kim SH, Kang MJ, Ishibe S, Young LH, Kotenko S, Compton S, Wilen CB, Wang A, Dela Cruz CS. Distinct Roles of Type I and Type III Interferons during a Native Murine β Coronavirus Lung Infection. Journal Of Virology 2021, 96: e01241-21. PMID: 34705554, PMCID: PMC8791255, DOI: 10.1128/jvi.01241-21.Peer-Reviewed Original ResearchConceptsType I interferonType III interferonsI interferonIII interferonsCoronavirus infectionInterferon deficiencyViral clearanceViral loadLung infectionType IHealthy young patientsImproved host survivalHost survivalRole of interferonMurine coronavirus infectionMajor health care threatViral burdenYounger patientsEarly diseaseIntranasal routeInterferon treatmentSublethal infectionEarly treatmentLethal infectionTissue injury
2020
Anti-Cancer Effects of RAW 264.7 Cells on Prostate Cancer PC-3 Cells.
Nam H, Bae J, Kim Y, An H, Kim S, Kim K, Yu S, Park B, Lee S, Ahn S. Anti-Cancer Effects of RAW 264.7 Cells on Prostate Cancer PC-3 Cells. Annals Of Clinical & Laboratory Science 2020, 50: 739-746. PMID: 33334788.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CommunicationCell Line, TumorCell MovementCoculture TechniquesCulture Media, ConditionedEpithelial-Mesenchymal TransitionHumansImmunotherapy, AdoptiveLipopolysaccharidesMacrophagesMaleMiceNeoplasm InvasivenessPC-3 CellsProstatic NeoplasmsRAW 264.7 CellsTumor MicroenvironmentConceptsPC-3 cellsAnti-cancer effectsProstate cancer PC-3 cellsCancer PC-3 cellsRAW 264.7 cellsTumor cellsHuman prostate cancer PC-3 cellsEMT-specific markersHigher anti-cancer effectEnzyme-linked immunosorbent assayQuantitative polymerase chain reactionAnti-cancer agentsPolymerase chain reactionImmune cellsInhibitor of metastasisTumor parametersTherapeutic targetingTGF-β2Snail-1Mesenchymal transitionTumor microenvironmentMigration markersWestern blotImmunosorbent assayAngiogenic ability
2018
Impact of Cigarette Smoke Exposure on the Lung Fibroblastic Response after Influenza Pneumonia
Lee SW, Sharma L, Kang YA, Kim SH, Chandrasekharan S, Losier A, Brady V, Bermejo S, Andrews N, Yoon CM, Liu W, Lee JY, Kang MJ, Dela Cruz CS. Impact of Cigarette Smoke Exposure on the Lung Fibroblastic Response after Influenza Pneumonia. American Journal Of Respiratory Cell And Molecular Biology 2018, 59: 770-781. PMID: 30110182, PMCID: PMC6293077, DOI: 10.1165/rcmb.2018-0004oc.Peer-Reviewed Original ResearchConceptsCigarette smoke exposureLungs of miceInfluenza infectionInfluenza virusBAL fluidSmoke exposureGrowth factor-β1 levelsAir-exposed lungsInfluenza-infected miceSignificant lung injuryFibroblastic responseLung-derived fibroblastsProtein-positive cellsGrowth factor-β1Influenza pneumoniaDifferent time pointsLung injurySmoking groupSignificant morbidityCS exposureMurine modelFibrotic responseΒ1 levelsFactor-β1Weight recoverySalinomycin ameliorates oxidative hepatic damage through AMP-activated protein kinase, facilitating autophagy
Kim K, Lee S, Baek S, Lee E, Jang E, Lee J, Ahn S, Chang J, Oh T, Kim S, Ma J, Kim S, Park K, Kim Y. Salinomycin ameliorates oxidative hepatic damage through AMP-activated protein kinase, facilitating autophagy. Toxicology And Applied Pharmacology 2018, 360: 141-149. PMID: 30290169, DOI: 10.1016/j.taap.2018.10.002.Peer-Reviewed Original ResearchConceptsImportance of AMPKMitochondrial dysfunctionAcidic vesicle organellesOxidative stressProtein kinase activationReactive oxygen species productionProtein kinaseAMPK inhibitionKinase activationSevere oxidative stressOxygen species productionLiver injuryMolecular mechanismsIron-induced apoptosisHepatic protectantStreptomyces albusHuman diseasesAMPKCellular mechanismsLC3-IISalinomycin's effectsArachidonic acidMitochondrial impairmentROS productionAnti-cancer agentsDeoxypodophyllotoxin in Anthriscus sylvestris alleviates fat accumulation in the liver via AMP-activated protein kinase, impeding SREBP-1c signal
Kim KY, Park KI, Lee SG, Baek SY, Lee EH, Kim S, Kim SH, Park SG, Yu SN, Oh TW, Kim JH, Kim KJ, Ahn SC, Kim Y. Deoxypodophyllotoxin in Anthriscus sylvestris alleviates fat accumulation in the liver via AMP-activated protein kinase, impeding SREBP-1c signal. Chemico-Biological Interactions 2018, 294: 151-157. PMID: 30148990, DOI: 10.1016/j.cbi.2018.08.025.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsApiaceaeBody WeightCholesterolDiet, High-FatDrugs, Chinese HerbalHep G2 CellsHumansHydrocarbons, FluorinatedLipid MetabolismLiverLiver X ReceptorsMaleMiceMice, Inbred C57BLPodophyllotoxinSignal TransductionSterol Regulatory Element Binding Protein 1SulfonamidesTriglyceridesUp-RegulationConceptsSREBP-1c inhibitionLipogenic genesDysregulation of AMPKAnthriscus sylvestrisProtein kinase activationSREBP-1c inductionHigh-fat dietSREBP-1cAcetyl-CoA carboxylaseSterol regulatory elementFatty acid synthaseTranscription factorsProtein kinaseRegulatory elementsStearoyl-CoA desaturase-1X receptor αLiver X receptor αAMPK activationKinase activationRegulatory mechanismsWild chervilOil Red O stainingFat dietAcid synthaseRed O staining
2017
Inhibition of Autophagy Promotes Salinomycin-Induced Apoptosis via Reactive Oxygen Species-Mediated PI3K/AKT/mTOR and ERK/p38 MAPK-Dependent Signaling in Human Prostate Cancer Cells
Kim K, Park K, Kim S, Yu S, Park S, Kim Y, Seo Y, Ma J, Ahn S. Inhibition of Autophagy Promotes Salinomycin-Induced Apoptosis via Reactive Oxygen Species-Mediated PI3K/AKT/mTOR and ERK/p38 MAPK-Dependent Signaling in Human Prostate Cancer Cells. International Journal Of Molecular Sciences 2017, 18: 1088. PMID: 28524116, PMCID: PMC5454997, DOI: 10.3390/ijms18051088.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAutophagyCell ProliferationExtracellular Signal-Regulated MAP KinasesFlavonoidsHumansImidazolesMaleMAP Kinase Signaling SystemMembrane Potential, MitochondrialP38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsProto-Oncogene Proteins c-aktPyransPyridinesReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsExtracellular signal-regulated kinasePI3K/Akt/mTORProstate cancer cellsAkt/mTORCancer cellsP38 MAPK-dependent signalingUpregulation of ERKSignal-regulated kinaseMAPK-dependent signalingP38 MAPK signaling pathwaysMitochondrial membrane potentialMAPK signaling pathwaysChemo-resistant cancersHuman prostate cancer cellsReactive oxygen species productionInhibition of autophagyPI3K inhibitorsPotential antitumor mechanismAcridine orange stainingCaspase-3 activityOxygen species productionSignaling pathwaysP38 inhibitorPropidium iodide assayMTOR activityLasalocid induces cytotoxic apoptosis and cytoprotective autophagy through reactive oxygen species in human prostate cancer PC-3 cells
Kim K, Kim S, Yu S, Park S, Kim Y, Nam H, An H, Yu H, Kim Y, Ji J, Seo Y, Ahn S. Lasalocid induces cytotoxic apoptosis and cytoprotective autophagy through reactive oxygen species in human prostate cancer PC-3 cells. Biomedicine & Pharmacotherapy 2017, 88: 1016-1024. PMID: 28178613, DOI: 10.1016/j.biopha.2017.01.140.Peer-Reviewed Original ResearchConceptsProstate cancer cellsHuman prostate cancer cellsAcidic vesicular organelles (AVOs) formationApoptotic cell death pathwayCancer cellsCell death pathwaysProtein 1 light chain 3Cell cycle arrestLight chain 3Human prostate cancer PC-3 cellsProstate cancer PC-3 cellsReactive oxygen species productionPossible signal pathwaysCancer PC-3 cellsInhibition of autophagyOrganelle formationDeath pathwaysNew potential chemotherapeutic agentsReactive oxygen speciesOxygen species productionMitochondrial hyperpolarizationMolecular mechanismsPotential chemotherapeutic agentProduction of ROSCytoprotective autophagy
2016
Monensin Induces PC-3 Prostate Cancer Cell Apoptosis via ROS Production and Ca2+ Homeostasis Disruption
KIM S, KIM K, YU S, PARK S, YU H, SEO Y, AHN S. Monensin Induces PC-3 Prostate Cancer Cell Apoptosis via ROS Production and Ca2+ Homeostasis Disruption. Anticancer Research 2016, 36: 5835-5843. PMID: 27793906, DOI: 10.21873/anticanres.11168.Peer-Reviewed Original ResearchConceptsCell cycle arrestApoptosis-related proteinsCell cycleCancer cellsPoly ADP-ribose polymerase (PARP) cleavageADP-ribose polymerase cleavageDisruption of CaProstate cancer cell apoptosisRibose polymerase cleavagePhase cell cycle arrestHuman prostate cancer cellsCell viabilityReactive oxygen species productionCancer cell apoptosisAnticancer effectsOxygen species productionMitochondrial ROS inhibitorProduction of ROSMitochondrial ROSDependent apoptosisProstate cancer cellsProstate cell linesPolymerase cleavageCell growth inhibitionPC-3 cellsToyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells
Park S, Kim S, Kim K, Yu S, Choi H, Kim Y, Nam H, Seo Y, Ahn S. Toyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells. Pharmacological Reports 2016, 69: 90-96. PMID: 27912102, DOI: 10.1016/j.pharep.2016.10.014.Peer-Reviewed Original ResearchConceptsProstate cancer PC-3 cellsPC-3 cellsCancer PC-3 cellsOxygen species productionROS productionERK MAPKNon-malignant RWPE-1 cellsExtracellular signal-regulated kinase (ERK) activityMitochondrial dysfunctionSpecies productionMitochondrial membrane potentialCell cycle arrestHuman prostate cancer PC-3 cellsReactive oxygen species productionCell viabilityRWPE-1 cellsProtein kinaseStreptomyces speciesKinase activityReactive oxygen speciesERK activationMolecular mechanismsP38 activationMAPK proteinsApoptotic effectsSilibinin induces mitochondrial NOX4-mediated endoplasmic reticulum stress response and its subsequent apoptosis
Kim S, Kim K, Yu S, Seo Y, Chun S, Yu H, Ahn S. Silibinin induces mitochondrial NOX4-mediated endoplasmic reticulum stress response and its subsequent apoptosis. BMC Cancer 2016, 16: 452. PMID: 27405931, PMCID: PMC4942927, DOI: 10.1186/s12885-016-2516-6.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesNOX4 expressionDisruption of Ca2ER stress responseProstate cancer PC-3 cellsCancer PC-3 cellsRegulation of NOX4Inhibited tumor growthCell linesPC-3 cellsProstate cell linesSilibinin-induced apoptosisProduction of ROSEndoplasmic reticulum stress responseExpression of apoptosisCancer cell linesProstate cancerROS-dependent apoptosisChemopreventive effectsMitochondrial Nox4Mitochondrial reactive oxygen speciesReticulum stress responseTumor growthFlow cytometryMethodsThe effects
2015
Autophagy inhibition enhances silibinin-induced apoptosis by regulating reactive oxygen species production in human prostate cancer PC-3 cells
Kim S, Kim K, Yu S, Park S, Choi H, Ji J, Ahn S. Autophagy inhibition enhances silibinin-induced apoptosis by regulating reactive oxygen species production in human prostate cancer PC-3 cells. Biochemical And Biophysical Research Communications 2015, 468: 151-156. PMID: 26522224, DOI: 10.1016/j.bbrc.2015.10.143.Peer-Reviewed Original ResearchConceptsPC-3 cellsProstate cancer PC-3 cellsCancer PC-3 cellsAcidic vesicular organellesSilibinin-induced apoptosisReactive oxygen speciesAnticancer effectsHuman prostate cancer PC-3 cellsMicrotubule-associated protein 1 light chain 3Inhibition of ROSProtein 1 light chain 3Major bioactive componentsInhibition of autophagyReactive oxygen species productionCancer cell linesLight chain 3Supportive therapyLiver conditionsOxygen species productionCancer modelProtective roleSilibininSilibinin-induced autophagyROS inhibitorChain 3
2013
Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin
Kim K, Cho H, Yu S, Kim S, Yu H, Park Y, Mirkheshti N, Kim S, Song C, Chatterjee B, Ahn S. Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin. Journal Of Cellular Biochemistry 2013, 114: 1124-1134. PMID: 23192945, DOI: 10.1002/jcb.24455.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBcl-2-Associated X ProteinCalciumCaspase 3Cell Line, TumorCell ProliferationCell SurvivalCytochromes cDrug Screening Assays, AntitumorDrugs, Chinese HerbalEgtazic AcidEnzyme ActivationG2 Phase Cell Cycle CheckpointsHomeostasisHumansIntracellular SpaceM Phase Cell Cycle CheckpointsMaleMembrane Potential, MitochondrialMitochondriaModels, BiologicalPodophyllotoxinProstatic NeoplasmsProtein TransportReactive Oxygen SpeciesConceptsReactive oxygen speciesMitochondrial homeostasisProstate cancer cellsCaspase-3-dependent pathwayCytochrome c releasePC-3 cell modelBax protein translocationTurn induced apoptosisCancer cellsMitochondrial membrane potentialOxygen speciesCaspase-3 activationN-acetylcysteineIncrease of ROSProtein translocationProstate cancerHuman prostate cancer cellsGeneration of ROSC releaseAntioxidant N-acetylcysteineNew anti-neoplastic agentsROS accumulationMitochondrial functionRecurrent prostate cancerLimited treatment options
2012
Pipernonaline from Piper longum Linn. induces ROS-mediated apoptosis in human prostate cancer PC-3 cells
Lee W, Kim K, Yu S, Kim S, Chun S, Ji J, Yu H, Ahn S. Pipernonaline from Piper longum Linn. induces ROS-mediated apoptosis in human prostate cancer PC-3 cells. Biochemical And Biophysical Research Communications 2012, 430: 406-412. PMID: 23159637, DOI: 10.1016/j.bbrc.2012.11.030.Peer-Reviewed Original ResearchConceptsPC-3 cellsHuman prostate cancer PC-3 cellsProstate cancer PC-3 cellsCancer PC-3 cellsReactive oxygen speciesAndrogen-independent PC-3LNCaP prostate cellsCaspase-3 assay kitHuman prostate cancerProstate cancer cellsIncrease of intracellularInduces reactive oxygen speciesTime-dependent mannerProstate cancerPiper longum LinnCleavage of PARPProstate cellsN-acetylcysteineAntiproliferation effectCyclin D1Caspase-3 activationAssay KitBcl-2 proteinCandidate agentCancer cells