Inhibition of Autophagy Promotes Salinomycin-Induced Apoptosis via Reactive Oxygen Species-Mediated PI3K/AKT/mTOR and ERK/p38 MAPK-Dependent Signaling in Human Prostate Cancer Cells
Kim K, Park K, Kim S, Yu S, Park S, Kim Y, Seo Y, Ma J, Ahn S. Inhibition of Autophagy Promotes Salinomycin-Induced Apoptosis via Reactive Oxygen Species-Mediated PI3K/AKT/mTOR and ERK/p38 MAPK-Dependent Signaling in Human Prostate Cancer Cells. International Journal Of Molecular Sciences 2017, 18: 1088. PMID: 28524116, PMCID: PMC5454997, DOI: 10.3390/ijms18051088.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAutophagyCell ProliferationExtracellular Signal-Regulated MAP KinasesFlavonoidsHumansImidazolesMaleMAP Kinase Signaling SystemMembrane Potential, MitochondrialP38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsProto-Oncogene Proteins c-aktPyransPyridinesReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsExtracellular signal-regulated kinasePI3K/Akt/mTORProstate cancer cellsAkt/mTORCancer cellsP38 MAPK-dependent signalingUpregulation of ERKSignal-regulated kinaseMAPK-dependent signalingP38 MAPK signaling pathwaysMitochondrial membrane potentialMAPK signaling pathwaysChemo-resistant cancersHuman prostate cancer cellsReactive oxygen species productionInhibition of autophagyPI3K inhibitorsPotential antitumor mechanismAcridine orange stainingCaspase-3 activityOxygen species productionSignaling pathwaysP38 inhibitorPropidium iodide assayMTOR activityDeoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells
Kim S, Son K, Kim K, Yu S, Park S, Kim Y, Nam H, Suh J, Ji J, Ahn S. Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells. Pharmacological Reports 2017, 69: 878-884. PMID: 28623712, DOI: 10.1016/j.pharep.2017.04.007.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, PhytogenicApoptosisAutophagyBone NeoplasmsCell Line, TumorCell SurvivalDrugs, Chinese HerbalGene Expression RegulationHumansOsteosarcomaPhosphoinositide-3 Kinase InhibitorsPodophyllotoxinProto-Oncogene Proteins c-aktReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsReactive oxygen speciesPI3K/AKT/mTOR pathwayAKT/mTOR pathwayU2OS cellsAcidic vesicular organelles (AVOs) formationMTOR pathwayMitochondrial reactive oxygen speciesOsteosarcoma U2OS cellsCell death modeOxygen speciesPropidium iodidePI3K/Akt/mTORCell cycle arrestLight chain 3Cell viabilityCellular processesInhibition of apoptosisAkt/mTOROrganelle formationAcridine orange stainingAutophagy processApoptotic responseDeath modeWestern blot analysisCell survival