2022
OAB-033: Pre-clinical models of genetically heterogeneous multiple myeloma reveal mechanisms of immune escape and predict clinical immunotherapy outcomes
Larrayoz M, Garcia-Barchino M, Celay J, Etxebeste A, Jimenez M, Perez C, Ordoñez R, Cobaleda C, Chesi M, Bergsagel L, Rodríguez-Otero P, Takahashi S, Katz S, Walensky L, Ruppert S, Lasater E, Amann M, Lasarte J, Kurilovich A, Cerchietti L, Agirre X, San-Miguel J, Paiva B, Prosper F, Martinez-Climent J. OAB-033: Pre-clinical models of genetically heterogeneous multiple myeloma reveal mechanisms of immune escape and predict clinical immunotherapy outcomes. Clinical Lymphoma Myeloma & Leukemia 2022, 22: s19-s20. DOI: 10.1016/s2152-2650(22)00306-8.Peer-Reviewed Original Research
2020
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
Boisvert E, Means R, Michaud M, Thomson J, Madri J, Katz S. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 DOI: 10.3791/60379-v.Peer-Reviewed Case Reports and Technical Notes
2019
A versatile flow-based assay for immunocyte-mediated cytotoxicity
Rabinovich PM, Zhang J, Kerr SR, Cheng BH, Komarovskaya M, Bersenev A, Hurwitz ME, Krause DS, Weissman SM, Katz SG. A versatile flow-based assay for immunocyte-mediated cytotoxicity. Journal Of Immunological Methods 2019, 474: 112668. PMID: 31525367, PMCID: PMC6891822, DOI: 10.1016/j.jim.2019.112668.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorCell NucleusCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicFlow CytometryHigh-Throughput Screening AssaysHumansImmunotherapy, AdoptiveKiller Cells, NaturalLymphocytes, Tumor-InfiltratingMaleMelanomaMice, Inbred C57BLPredictive Value of TestsReceptors, Chimeric AntigenReproducibility of ResultsSkin NeoplasmsT-LymphocytesTime FactorsWorkflowConceptsCell-mediated cytotoxicityTumor-Infiltrating LymphocytesEffector cellsTarget cellsNK-92 cellsChimeric antigen receptorNuclear staining patternInfiltrating lymphocytesT cellsEffector nucleiFlow-based assayImmune systemFlow cytometryStaining patternAntigen receptorDead cellsKilling reactionCytotoxicityCell permeable dyeCellsAssaysCell mixturesNuclear proteinsNovel strategyCell proteinsProapoptotic protein BIM as a novel prognostic marker in mantle cell lymphoma
Wang JD, Katz SG, Morgan EA, Yang DT, Pan X, Xu ML. Proapoptotic protein BIM as a novel prognostic marker in mantle cell lymphoma. Human Pathology 2019, 93: 54-64. PMID: 31425695, PMCID: PMC7038910, DOI: 10.1016/j.humpath.2019.08.008.Peer-Reviewed Original ResearchConceptsMantle cell lymphomaCell lymphomaAnn Arbor stage IIINovel independent prognostic factorAggressive B-cell lymphomasHigh Bim expressionAverage patient ageBim expressionIndependent prognostic factorLarge academic medical centerKi-67 indexNovel prognostic markerB-cell lymphomaAcademic medical centerCyclin D1 overexpressionHuman mantle cell lymphomaMCL cohortMIPI scoreProgressive diseaseComplete responseOverall survivalPatient agePrognostic factorsTumor cell survivalFemale ratioBOK promotes erythropoiesis in a mouse model of myelodysplastic syndrome
Kang SH, Perales O, Michaud M, Katz SG. BOK promotes erythropoiesis in a mouse model of myelodysplastic syndrome. Annals Of Hematology 2019, 98: 2089-2096. PMID: 31203423, PMCID: PMC6702064, DOI: 10.1007/s00277-019-03726-7.Peer-Reviewed Original ResearchConceptsUnfolded protein responseER stressPro-apoptotic membersBcl-2 familyNHD13 miceRT-qPCR analysisInduction of apoptosisProgenitor stem cellsHematopoietic progenitor cell assaysProtein responseDownstream effectorsGene knockoutMyelodysplastic syndromeCell stressProgenitor cell assaysEndoplasmic reticulumLower mean cell hemoglobin concentrationErythroid progenitorsNUP98-HOXD13 transgenic miceClonal hematopoietic stem cell disordersStem cellsSimilar overall survivalAcute myeloid leukemiaHematopoietic stem cell disordersMean cell hemoglobin concentrationMinocycline mitigates the effect of neonatal hypoxic insult on human brain organoids
Boisvert EM, Means RE, Michaud M, Madri JA, Katz SG. Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids. Cell Death & Disease 2019, 10: 325. PMID: 30975982, PMCID: PMC6459920, DOI: 10.1038/s41419-019-1553-x.Peer-Reviewed Original ResearchConceptsNeonatal hypoxic injuryBrain developmentEfficacy of minocyclineLow birth weightUse of minocyclineEffects of hypoxiaNormal brain developmentCerebral organoid modelHuman brain organoidsLater time pointsAnimal model systemsNeonatal hypoxicDevastating causeCerebral palsySignificant morbidityHuman brain developmentNeurological consequencesBirth weightHypoxic injuryNeuronal deathCortical neuronsInjury resultsGlial cellsForebrain markersPotential treatment
2018
Methods to Probe Calcium Regulation by BCL-2 Family Members
Carpio MA, Katz SG. Methods to Probe Calcium Regulation by BCL-2 Family Members. Methods In Molecular Biology 2018, 1877: 173-183. PMID: 30536006, DOI: 10.1007/978-1-4939-8861-7_12.Peer-Reviewed Original ResearchConceptsMitochondrial outer membrane permeabilizationBcl-2 family membersEndoplasmic reticulumOuter membrane permeabilizationProcess of apoptosisSuch important functionsMembrane permeabilizationDirect regulationFamily membersAdditional roleImportant functionsCalcium regulationApoptosisRegulationRelease of calciumMitochondriaPermeabilizationBiologyCytosolMembersReticulum
2017
Bok Promotes Erythropoiesis in a Mouse Model of Myelodysplastic Syndrome
Kang S, Perales O, Michuad M, Katz S. Bok Promotes Erythropoiesis in a Mouse Model of Myelodysplastic Syndrome. Blood 2017, 130: 922. DOI: 10.1182/blood.v130.suppl_1.922.922.Peer-Reviewed Original ResearchAcute myeloid leukemiaNHD13 miceMean cell hemoglobin concentrationMouse modelBone marrowMean cell volumeMDS/acute myeloid leukemiaRBC progenitorsUnfolded protein responseSimilar overall survivalTransgenic mouse modelPathogenesis of myelodysplasiaER stressHigher mean cell volumeLower mean cell hemoglobin concentrationCell hemoglobin concentrationAnti-apoptotic Bcl-2Endoplasmic reticulum stressOverall survivalProgenitor stem cellsRed blood cellsLower hemoglobinMyelodysplastic syndromeMyeloid leukemiaRegulation of erythropoiesisEZH! The IRE of DLBCL gets an UPR hand
Katz SG. EZH! The IRE of DLBCL gets an UPR hand. Blood 2017, 129: 2340-2342. PMID: 28450573, DOI: 10.1182/blood-2017-02-769067.Peer-Reviewed Original ResearchNon-apoptotic functions of BCL-2 family proteins
Gross A, Katz SG. Non-apoptotic functions of BCL-2 family proteins. Cell Death & Differentiation 2017, 24: 1348-1358. PMID: 28234359, PMCID: PMC5520452, DOI: 10.1038/cdd.2017.22.Peer-Reviewed Original ResearchConceptsNon-apoptotic rolesBcl-2 family proteinsFamily proteinsApoptotic roleNon-apoptotic functionsWhole-cell metabolismCellular survival pathwaysMitochondrial physiologyCellular survivalSurvival pathwaysMajor regulatorNuclear processesApoptosis processProteinMechanism of actionPhysiologyImportant cluesRoleAutophagyRegulatorFascinating fieldRegulationPathwayMechanismMetabolism
2016
Expression of CD30 as a biomarker to predict response to brentuximab vedotin
Xu ML, Acevedo-Gadea C, Seropian S, Katz SG. Expression of CD30 as a biomarker to predict response to brentuximab vedotin. Histopathology 2016, 69: 155-158. PMID: 26648051, PMCID: PMC7064871, DOI: 10.1111/his.12914.Peer-Reviewed Original Research
2015
SOX11 Cooperates with CCND1 in Mantle Cell Lymphoma Pathogenesis
Kuo P, Jiang Z, Perumal D, Leshchenko V, Lagana' A, Katz S, Walensky L, Shaknovich R, Ye B, Parekh S. SOX11 Cooperates with CCND1 in Mantle Cell Lymphoma Pathogenesis. Blood 2015, 126: 1253. DOI: 10.1182/blood.v126.23.1253.1253.Peer-Reviewed Original ResearchTransgenic mouse modelB cell populationsMouse modelB cellsBone marrowTransgenic micePeripheral bloodPeritoneal cavitySOX11 expressionDouble transgenic mouse modelIncurable B-cell malignancySingle transgenic miceB-cell hyperplasiaB-cell phenotypeWild-type miceCell populationsB-cell malignanciesRole of SOX11Multiple oncogenic pathwaysCyclin D1 overexpressionMCL pathogenesisMCL tumorsExpression of SOX11Key diagnostic featuresMedian survivalBCL-2 family member BOK promotes apoptosis in response to endoplasmic reticulum stress
Carpio MA, Michaud M, Zhou W, Fisher JK, Walensky LD, Katz SG. BCL-2 family member BOK promotes apoptosis in response to endoplasmic reticulum stress. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 7201-7206. PMID: 26015568, PMCID: PMC4466744, DOI: 10.1073/pnas.1421063112.Peer-Reviewed Original ResearchConceptsB-cell lymphoma 2 ovarian killerApoptotic defectsMultidomain proapoptotic proteins BaxApoptotic responseStress stimuliER stressBcl-2 family proteinsER stress agentsUnfolded protein responseMouse embryonic fibroblastsDefective apoptotic responseMitochondrial apoptotic pathwayProapoptotic protein BaxPredominant subcellular localizationThapsigargin-induced apoptosisEndoplasmic reticulum stressFamily proteinsDeath responseSubcellular localizationEmbryonic fibroblastsHigh homologyProtein responseApoptotic pathwayOvert phenotypeProtein Bax
2014
Synthesis of Stabilized Alpha-Helical Peptides
Bernal F, Katz SG. Synthesis of Stabilized Alpha-Helical Peptides. Methods In Molecular Biology 2014, 1176: 107-114. PMID: 25030922, PMCID: PMC6333094, DOI: 10.1007/978-1-4939-0992-6_9.Peer-Reviewed Original ResearchConceptsAlpha-helical peptidesImportant protein-protein interactionsSpecific amino acid residuesProtein-protein interactionsAlpha-helical motifOlefin metathesis reactionsAlpha-helical structureRing-closing olefin metathesis reactionAmino acid residuesPosttranslational modificationsProtein interactionsMetathesis reactionHost proteinsProtein targetsAcid residuesCrystal structureSecondary structureFunctional consequencesRemarkable stabilitySmall moleculesMost peptidesInteraction sitesStructural studiesCell permeabilitySynthesisDistinct BimBH3 (BimSAHB) Stapled Peptides for Structural and Cellular Studies
Bird GH, Gavathiotis E, LaBelle JL, Katz SG, Walensky LD. Distinct BimBH3 (BimSAHB) Stapled Peptides for Structural and Cellular Studies. ACS Chemical Biology 2014, 9: 831-837. PMID: 24358963, PMCID: PMC4131438, DOI: 10.1021/cb4003305.Peer-Reviewed Original Research
2013
Mantle cell lymphoma in cyclin D1 transgenic mice with Bim-deficient B cells
Katz SG, LaBelle JL, Meng H, Valeriano RP, Fisher JK, Sun H, Rodig SJ, Kleinstein SH, Walensky LD. Mantle cell lymphoma in cyclin D1 transgenic mice with Bim-deficient B cells. Blood 2013, 123: 884-893. PMID: 24352880, PMCID: PMC3916879, DOI: 10.1182/blood-2013-04-499079.Peer-Reviewed Original ResearchConceptsMantle cell lymphomaCyclin D1 transgenic miceCyclin D1 overexpressionB cellsCell lymphomaAggressive B-cell lymphomasSubset of miceTransgenic mouse modelB-cell lymphomaDeletion of BimPathogenesis of MCLHuman mantle cell lymphomaDevelopment of MCLStimulation regimensConventional chemotherapyMouse modelLymphoid maturationTransgenic miceLymphomaBIM deletionSelective expansionMiceProapoptotic BimPathogenesisGenetic aberrations
2012
Brain and testicular tumors in mice with progenitor cells lacking BAX and BAK
Katz SG, Fisher JK, Correll M, Bronson RT, Ligon KL, Walensky LD. Brain and testicular tumors in mice with progenitor cells lacking BAX and BAK. Oncogene 2012, 32: 4078-4085. PMID: 22986529, PMCID: PMC3529761, DOI: 10.1038/onc.2012.421.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsProapoptotic Bcl-2 family proteins BaxSubventricular zoneBcl-2 family proteins BaxProgenitor cellsLong-term pathophysiological consequencesMitochondrial outer membraneBax/BakDeletion of BaxStem cell nicheHigh-grade tumorsMultiple developmental abnormalitiesIntrinsic apoptotic pathwayOlfactory bulb regionNestin-positive cellsProgenitor cell poolCellular excessDeath stimuliLoss of functionHypercellular tumorOuter membraneTesticular tumorsCell nicheLong-term regulationApoptotic pathwayA stapled BIM peptide overcomes apoptotic resistance in hematologic cancers
LaBelle JL, Katz SG, Bird GH, Gavathiotis E, Stewart ML, Lawrence C, Fisher JK, Godes M, Pitter K, Kung AL, Walensky LD. A stapled BIM peptide overcomes apoptotic resistance in hematologic cancers. Journal Of Clinical Investigation 2012, 122: 2018-2031. PMID: 22622039, PMCID: PMC3366394, DOI: 10.1172/jci46231.Peer-Reviewed Original ResearchConceptsBcl-2 family proteinsCell deathFamily proteinsPro-apoptotic Bcl-2 family proteinsBcl-2 protein familyBcl-2-interacting mediatorBcl-2 family pathwayHydrocarbon-stapled peptidesCell death mechanismsCancer cellsCellular lifeHematologic cancer cellsProtein familyDeath domainBH3 helixBim BH3Bim peptidesDeath mechanismsApoptotic blockadesApoptotic resistanceFamily pathwaySurvival pathwaysAntiapoptotic proteinsProapoptotic activityHuman AML xenograft model
2010
A Stapled BIM BH3 Helix Restores Apoptosis In Bim-Null Mantle Cell Lymphoma
Katz S, Labelle J, Godes M, Fisher J, Bird G, Walensky L. A Stapled BIM BH3 Helix Restores Apoptosis In Bim-Null Mantle Cell Lymphoma. Blood 2010, 116: 437. DOI: 10.1182/blood.v116.21.437.437.Peer-Reviewed Original ResearchMantle cell lymphomaCell lymphomaAggressive B-cell lymphomasDevelopment of splenomegalyRAG2-deficient miceB-cell lymphomaLymphoproliferative diseaseOrgan infiltrationMCL cell linesConventional chemotherapyHematologic cancersAnti-apoptotic proteinsBone marrowNormal parenchymaVivo modelPro-apoptotic proteinsCell-permeable peptideLymphomaPro-apoptotic activityGenetic aberrationsMiceBcl-2TUNEL positivityPro-apoptotic Bcl-2 family protein BimCaspase-3/7
2008
Structural Analysis of a BAX-BIM SAHB Complex Reveals a Novel BH3 Interaction Site on BAX for Therapeutic Activation of Apoptosis
Gavathiotis E, Suzuki M, Davis M, Pitter K, Bird G, Katz S, Tu H, Kim H, Cheng E, Tjandra N, Walensky L. Structural Analysis of a BAX-BIM SAHB Complex Reveals a Novel BH3 Interaction Site on BAX for Therapeutic Activation of Apoptosis. Blood 2008, 112: 300. DOI: 10.1182/blood.v112.11.300.300.Peer-Reviewed Original ResearchAnti-apoptotic proteinsBax activationCell deathInteraction sitesAlpha-helixBCL-2 domainsBcl-2 family proteinsBcl-2 familyPathologic cell deathBax-mediated apoptosisFirst structural analysisPro-apoptotic proteinsDomain proteinsFamily proteinsMitochondrial translocationProtein interactionsPoint mutagenesisMitochondrial apoptosisStress stimuliBcl-xLConformational changesCell survivalMitochondrial dysfunctionNovel siteDistinct assays