Synthetic Metallochaperone ZMC1 Rescues Mutant p53 Conformation by Transporting Zinc into Cells as an Ionophore
Blanden AR, Yu X, Wolfe AJ, Gilleran JA, Augeri DJ, O'Dell RS, Olson EC, Kimball SD, Emge TJ, Movileanu L, Carpizo DR, Loh SN. Synthetic Metallochaperone ZMC1 Rescues Mutant p53 Conformation by Transporting Zinc into Cells as an Ionophore. Molecular Pharmacology 2015, 87: 825-831. PMID: 25710967, PMCID: PMC4407733, DOI: 10.1124/mol.114.097550.Peer-Reviewed Original ResearchConceptsHuman embryonic kidney cell line 293Mutant p53 conformationEssential zinc ionCell line 293Mutant proteinsP53-R175HPlasma membraneExtracellular environmentP53 conformationTumor suppressorLiving cellsHuman cancersBinding sitesX-ray crystal structureCommon mutationsObserved reactivationCellsZinc ionsMetallochaperoneVivo activityMutantsSuppressorOrganismsProteinR175H