Ex utero electroporation and whole hemisphere explants: a simple experimental method for studies of early cortical development.
Nichols AJ, O'Dell RS, Powrozek TA, Olson EC. Ex utero electroporation and whole hemisphere explants: a simple experimental method for studies of early cortical development. Journal Of Visualized Experiments 2013 PMID: 23609059, PMCID: PMC3643163, DOI: 10.3791/50271.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDNAElectroporationFemaleGreen Fluorescent ProteinsMaleMicePlasmidsPregnancyConceptsEarly cortical developmentCortical developmentExplant modelUtero electroporationNon-neuronal elementsPreplate splittingCortical slicesPharmacological interventionsNeuronal proliferationMedial cortexCellular laminationSurvival surgeryBlood vesselsExplant systemNeuronal differentiationPrecursor cellsSlice explantsNeuronsLive imaging approachesOrganotypic environmentImaging approachExtracellular matrixSurgeryMeningesCortex