2013
Ex utero electroporation and whole hemisphere explants: a simple experimental method for studies of early cortical development.
Nichols AJ, O'Dell RS, Powrozek TA, Olson EC. Ex utero electroporation and whole hemisphere explants: a simple experimental method for studies of early cortical development. Journal Of Visualized Experiments 2013 PMID: 23609059, PMCID: PMC3643163, DOI: 10.3791/50271.Peer-Reviewed Original ResearchConceptsEarly cortical developmentCortical developmentExplant modelUtero electroporationNon-neuronal elementsPreplate splittingCortical slicesPharmacological interventionsNeuronal proliferationMedial cortexCellular laminationSurvival surgeryBlood vesselsExplant systemNeuronal differentiationPrecursor cellsSlice explantsNeuronsLive imaging approachesOrganotypic environmentImaging approachExtracellular matrixSurgeryMeningesCortex
2012
Layer 6 cortical neurons require Reelin-Dab1 signaling for cellular orientation, Golgi deployment, and directed neurite growth into the marginal zone
O’Dell R, Ustine CJ, Cameron DA, Lawless SM, Williams RM, Zipfel WR, Olson EC. Layer 6 cortical neurons require Reelin-Dab1 signaling for cellular orientation, Golgi deployment, and directed neurite growth into the marginal zone. Neural Development 2012, 7: 25. PMID: 22770513, PMCID: PMC3466444, DOI: 10.1186/1749-8104-7-25.Peer-Reviewed Original ResearchConceptsEarly cortical developmentMutant cortexCortical neuronsCortical developmentPial surfaceMarginal zoneApical neuritesL6 neuronsRecombinant ReelinNeocortical neuronsNeuronal somataReelin-Dab1Arbor lengthArbor sizeExplant modelVentricular zonePioneer neuronsMutant neuronsReelinNeuronsConclusionsThese findingsMore primary processesNeuronal orientationCortexNeurite growth