2001
In Vitro Selection of Kinase and Ligase Deoxyribozymes
Li Y, Breaker R. In Vitro Selection of Kinase and Ligase Deoxyribozymes. Methods 2001, 23: 179-190. PMID: 11181037, DOI: 10.1006/meth.2000.1119.Peer-Reviewed Original ResearchConceptsDNA ligationModern living systemsDNA kinaseEnzymatic functionRandom sequence populationVitro SelectionProtein enzymesConstruction of DNADNA constructsDNA phosphorylationDNADeoxyribozymesKinaseLiving systemsFundamental roleChemical reactionsAdenylationFundamental questionsBiocatalysisPhosphorylationEnzymeBiotechnologySelection strategyDiscoveryDetailed overview
1999
Nucleic acid molecular switches
Soukup G, Breaker R. Nucleic acid molecular switches. Trends In Biotechnology 1999, 17: 469-476. PMID: 10557159, DOI: 10.1016/s0167-7799(99)01383-9.Peer-Reviewed Original ResearchDeoxyribozymes: New players in the ancient game of biocatalysis
Li Y, Breaker R. Deoxyribozymes: New players in the ancient game of biocatalysis. Current Opinion In Structural Biology 1999, 9: 315-323. PMID: 10361095, DOI: 10.1016/s0959-440x(99)80042-6.Peer-Reviewed Original ResearchConceptsSubstrate recognitionGenetic informationIdeal storage systemBiological catalysisRNA counterpartsInert characterChemical reactionsDNANovel chemicalRate enhancementSubstantial untapped potentialNew playersArtificial DNAHelical structurePolynucleotide chainSurprising varietyDeoxyribozymesRecent studiesStructure formationProteinCatalysisBiocatalysisStorage systemReactionChemicals
1994
Emergence of a replicating species from an in vitro RNA evolution reaction.
Breaker R, Joyce G. Emergence of a replicating species from an in vitro RNA evolution reaction. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 6093-6097. PMID: 7517040, PMCID: PMC44144, DOI: 10.1073/pnas.91.13.6093.Peer-Reviewed Original ResearchConceptsRNA polymeraseDNA-dependent RNA polymeraseRNA polymerase promoter sequencePromoter sequencesEvolution experimentsRNA moleculesAcid sequenceRNA transcriptsSpecific nucleic acid sequencesTarget chemical reactionNucleic acid sequencesRNA replicationPolymeraseRNADNAReverse transcriptaseSelective amplificationDNA hairpinsSequence replicationChemical reactionsUnique classReplicationSequenceTranscriptaseParticular chemical reaction